1.上海市松江区农业技术推广中心,上海 松江,201613
2.扬州大学农学院/江苏省作物栽培生理重点实验室/江苏省粮食作物现代产业技术协同创新中心,江苏 扬州,225100
3.上海佘农稻米专业合作社,上海 松江,201600
林国冰(2000- ),男,助理农艺师,硕士,研究方向为作物栽培及生理,E-mail: lgb1832659540@163.com
顾啸(1989- ),男,农艺师,硕士,主要从事作物育种及农业技术推广,E-mail: gx13sh@163.com
收稿:2026-08-07,
纸质出版:2026-10-25
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林国冰,金鑫,谢志奎等.外源赤霉素浸种缓解盐胁迫对油菜种子萌发及幼苗生理响应的抑制[J].中国油料作物学报,2026,48(05):101-113.
LIN Guo-bing,JIN Xin,XIE Zhi-kui,et al.Exogenous gibberellin seed soaking alleviates the inhibition of salt seress on rapeseed germination and seedling physiological responses[J].Chinese Journal of Oil Crop Sciences,2026,48(05):101-113.
林国冰,金鑫,谢志奎等.外源赤霉素浸种缓解盐胁迫对油菜种子萌发及幼苗生理响应的抑制[J].中国油料作物学报,2026,48(05):101-113. DOI: 10.19802/j.issn.1007-9084.2026271.
LIN Guo-bing,JIN Xin,XIE Zhi-kui,et al.Exogenous gibberellin seed soaking alleviates the inhibition of salt seress on rapeseed germination and seedling physiological responses[J].Chinese Journal of Oil Crop Sciences,2026,48(05):101-113. DOI: 10.19802/j.issn.1007-9084.2026271.
为探明外源赤霉素(GA
3
)浸种缓解盐胁迫抑制油菜种子萌发的作用及其生理响应,以耐盐性不同的油菜品种华油杂62和浙油50为材料,采用种子萌发试验结合生理指标测定的方法,研究GA
3
处理对盐胁迫下油菜萌发特性、幼苗生长、活性氧代谢、膜稳定性及渗透调节的影响。研究表明,150 mmol·L
-
¹ NaCl胁迫显著降低了油菜种子的萌发能力和幼苗生长水平,且浙油50受抑制程度高于华油杂62。外源GA
3
浸种能够显著缓解盐胁迫造成的萌发抑制。进一步研究发现,GA
3
通过增强SOD、POD和CAT等抗氧化酶活性,降低活性氧积累和膜脂过氧化程度,同时促进脯氨酸、可溶性糖和可溶性蛋白积累,提高油菜幼苗的渗透调节能力。综上,外源GA
3
通过调控氧化稳态和渗透平衡,改善盐胁迫下细胞稳态环境,促进油菜种子萌发恢复和幼苗正常建立。
This study aimed to elucidate the effects of exogenous gibberellic acid (GA₃) seed priming on alleviating salt stress-induced inhibition of rapeseed seed germination and its physiological mechanisms. Two rapeseed cultivars with contrasting salt tolerance
Huayouza 62 and Zheyou 50
were used as materials. Seed germination assays combined with physiological measurements were conducted to investigate the effects of GA₃ on germination characterist
ics
seedling growth
reactive oxygen species (ROS) metabolism
membrane stability
and osmotic adjustment under salt stress. The results showed that 150 mmol·L
-1
NaCl stress significantly inhibited seed germination and seedling growth in both cultivars
with Zheyou 50 exhibiting greater sensitivity to salt stress than Huayouza 62. Exogenous GA
3
seed soaking significantly alleviated the salt inhibition on germination. Further analyses showed that GA
3
enhanced the activities of antioxidant enzymes
including superoxide dismutase
peroxidase
and catalase
thereby reducing ROS accumulation and membrane lipid peroxidation. Meanwhile
GA
3
promoted the accumulation of proline
soluble sugars
and soluble proteins
thereby enhancing the osmotic adjustment capacity of rapeseed seedlings. Overall
exogenous GA
3
alleviated the adverse effects of salt stress on rapeseed seed germination and seedling establishment by improving redox homeostasis and osmotic balance
thereby contributing to the maintenance of cellular homeostasis under salt stress.
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