山西农业大学小麦研究所,山西 临汾,041000
杨秀丽(1980- ),女,副研究员,研究方向为花生种质资源创制及新品种选育,E-mail: yangxiuli1234@163.com
收稿:2026-01-08,
纸质出版:2026-10-25
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杨秀丽,宁东贤,张泽.花生半矮化突变体dwarf1对植物外源激素生理响应性分析[J].中国油料作物学报,2026,48(05):123-132.
YANG Xiu-li,NING Dong-xian,ZHANG Ze.Physiological responses of peanut semi-dwarf mutant to exogenous hormones[J].Chinese Journal of Oil Crop Sciences,2026,48(05):123-132.
杨秀丽,宁东贤,张泽.花生半矮化突变体dwarf1对植物外源激素生理响应性分析[J].中国油料作物学报,2026,48(05):123-132. DOI: 10.19802/j.issn.1007-9084.2026004.
YANG Xiu-li,NING Dong-xian,ZHANG Ze.Physiological responses of peanut semi-dwarf mutant to exogenous hormones[J].Chinese Journal of Oil Crop Sciences,2026,48(05):123-132. DOI: 10.19802/j.issn.1007-9084.2026004.
为给花生株型改良育种提供依据,本研究以花生半矮化突变体
dwarf1
和野生型WT为材料,设置外源赤霉素(GA
3
)、生长素(IAA)和油菜素内酯(BR)的梯度浓度处理,通过测定主茎高度、节数、节间长度及内源激素含量,系统解析半矮化突变体
dwarf1
矮化表型的生理机制。结果表明:
dwarf1
对外源GA₃响应极敏感,主茎高度、节数和节间长度随GA₃浓度升高而显著增加,10 mg·L⁻¹ GA₃处理可使其恢复至野生型水平,其内源GA₃含量极显著低于野生型。外源IAA处理对
dwarf1
与WT的主茎伸长无显著影响,但
dwarf1
内源IAA含量显著低于野生型,揭示IAA生物合成或信号转导通路可能存在异常。此外,
dwarf1
与WT的内源BR含量无显著差异,但低浓度外源BR处理对
dwarf1
的主茎伸长具有明显促进作用。综合上述结果推测,花生半矮化突变体
dwarf1
的矮化表型主要源于赤霉素生物合成途径受阻,并可能与生长素途径协同调控主茎生长,而油菜素内酯途径可能并非主要成因。
To advance the breeding of ideal peanut plant architecture
a semi-dwarf mutant
dwarf1
and its wild-type (WT) were treated with gradient concentrations of exogenous gibberellin (GA₃)
auxin (IAA)
and brassinosteroid (BR). The main stem height
node number
internode length
and endogenous hormone contents were systematically measured to elucidate physiological mechanism underlying the semi-dwarf phenotype of
dwarf1
. Results showed that
dwarf1
was highly sensitive to exogenous GA₃. The main stem height
node number
and internode length increased significantly with increasing GA₃ concentration
and treatment with 10 mg·L⁻¹ GA₃ restored these parameters to WT level. Its endogenous GA₃ content was extremely significantly lower than that in WT. Exogenous IAA treatment had no significant effect on main stem elongation in either
dwarf1
or WT
but the endogenous IAA content in
dwarf1
was significantly
reduced
suggesting possible abnormalities in IAA biosynthesis or signal transduction. Furthermore
there was no significant difference in endogenous BR content between
dwarf1
and WT
yet low-concentration exogenous BR treatment still had a clear promoting effect on main stem elongation in
dwarf1
. Based on these results
it is speculated that the semi-dwarf phenotype of peanut mutant
dwarf1
primarily results from a blockage in gibberellin biosynthesis pathway
possibly in coordination with auxin pathway to regulate main stem growth
while brassinosteroid pathway might not be the major contributing factor.
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