丰富环境对脑缺血再灌注大鼠神经功能及血管再生的影响

张怡欣; 章鑫; 陈修平; 廖维靖

doi:10.14188/j.1671-8852.2024.0857

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丰富环境对脑缺血再灌注大鼠神经功能及血管再生的影响

基础医学研究 | 更新时间：2026-11-06

- 丰富环境对脑缺血再灌注大鼠神经功能及血管再生的影响
- Effects of enriched environment on neurological function and angiogenesis in cerebral ischemia/reperfusion rats
- 武汉大学学报（医学版） 2025年46卷第12期页码：1544-1551
- 作者机构：
  
  1.武汉大学中南医院神经康复科湖北武汉 430071
  2.南昌大学第一附属医院康复医学科江西南昌 330006
- 作者简介：
  
  张怡欣，女，2000—，医学硕士生，主要从事神经康复方向的研究，E⁃mail:yixinzhang@whu.edu.cn
  廖维靖，男，1962—，医学博士，教授，博士生导师，主要从事神经康复方向的研究，E⁃mail:weijingliao@sina.com
- 基金信息：
  
  武汉大学中南医院学科培育项目(XKJS202034)
- DOI：10.14188/j.1671-8852.2024.0857
  中图分类号： R493
- 收稿：2024-11-07，
  
  纸质出版：2025-12-25
- 稿件说明：
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张怡欣,章鑫,陈修平等.丰富环境对脑缺血再灌注大鼠神经功能及血管再生的影响[J].武汉大学学报（医学版）,2025,46(12):1544-1551.

ZHANG Yixin,ZHANG Xin,CHEN Xiuping,et al.Effects of enriched environment on neurological function and angiogenesis in cerebral ischemia/reperfusion rats[J].Medical Journal of Wuhan University,2025,46(12):1544-1551.
张怡欣,章鑫,陈修平等.丰富环境对脑缺血再灌注大鼠神经功能及血管再生的影响[J].武汉大学学报（医学版）,2025,46(12):1544-1551. DOI： 10.14188/j.1671-8852.2024.0857.

ZHANG Yixin,ZHANG Xin,CHEN Xiuping,et al.Effects of enriched environment on neurological function and angiogenesis in cerebral ischemia/reperfusion rats[J].Medical Journal of Wuhan University,2025,46(12):1544-1551. DOI： 10.14188/j.1671-8852.2024.0857.

摘要

目的

探讨丰富环境（EE）对脑缺血再灌注损伤大鼠神经功能及血管再生的影响。

方法

将雄性SD大鼠分为假手术组（SS）、模型组（IS）、躯体性丰富环境组（PE）、社会性丰富环境组（SE）、经典丰富环境组（PSE），每组12只大鼠。IS、PE、SE、PSE组进行左侧大脑中动脉阻塞再灌注（MCAO/R）手术，SS组进行假手术。术后进行21 d环境干预，使用改良神经功能缺损评分（mNSS）评估神经功能，HE染色和尼氏染色观察病理变化，免疫组化和Western Blot检测促红细胞生成素（EPO）表达。

结果

①丰富环境改善了大鼠神经功能，PSE组效果最佳（

0.05）；②丰富环境减轻了神经元损伤，PSE组修复更明显（

0.05）；③丰富环境增加了EPO表达，PSE组EPO表达最高（

0.05）；④皮尔逊相关性分析显示mNSS评分与EPO表达显著相关（

0.05）。

结论

丰富环境可以促进大鼠脑缺血再灌注损伤后的神经功能恢复和神经元修复，其机制可能与上调EPO的表达有关。PSE组治疗效果优于PE和SE组，表明运动训练结合社交/认知刺激能更好地促进神经功能恢复。

Abstract

Objective

To investigate the effects of enriched environment (EE) on neurological function and angiogenesis in rats with cerebral ischemia/reperfusion injury.

Methods

Male Sprague⁃Dawley (SD) rats were divided into the sham⁃operated group (SS)

model group (IS)

physical environmental enrichment group (PE)

social environment enrichment group (SE)

and typical physical and social enrichment group (PSE). The IS

and PSE groups underwent left middle cerebral artery occlusion/reperfusion (MCAO/R) surgery and the SS group underwent sham surgery. Postoperative environmental interventions were performed for 21 days. Neurological function was assessed using the Modified Neurological Severity Score (mNSS)

pathological changes were observed by HE staining and Nissl staining

and Erythropoietin (EPO) expression was detected by immunohistochemistry and Western blot.

Results

① EE improved the neurological function of rats

and the PSE group showed the greatest improvement (

0.05); ② EE alleviated neuronal damage

and the repair was more pronounced in the PSE group (

0.05); ③ EE increased the expression of EPO

and the PSE group had the highest level (

0.05). ④ Pearson correlation analysis showed that mNSS scores were significantly correlated with EPO expression (

0.05).

Conclusion

EE promotes neurological function recovery and neuronal repair after cerebral ischemia/reperfusion injury in rats by a mechanism that may be related to the upregulation of EPO expression. The therapeutic effect of the PSE group was superior to that of the PE and SE groups

suggesting that the combination of physical exercise and social interaction can more effectively promote neurological recovery.

关键词

Keywords

references

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