阿克曼嗜黏液菌外膜蛋白Amuc_1100调控STING信号通路减轻小鼠肠缺血再灌注损伤的机制

朴光宇; 施瑜; 陈榕; 孟庆涛

doi:10.14188/j.1671-8852.2024.0674

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阿克曼嗜黏液菌外膜蛋白Amuc_1100调控STING信号通路减轻小鼠肠缺血再灌注损伤的机制

基础医学研究 | 更新时间：2026-11-05

- 阿克曼嗜黏液菌外膜蛋白Amuc_1100调控STING信号通路减轻小鼠肠缺血再灌注损伤的机制
- Mechanisms of outer membrane protein Amuc_1100 of Akkermansia muciniphila attenuating intestinal ischemia⁃reperfusion injury through STING signaling pathway in mice
- 武汉大学学报（医学版） 2025年46卷第7期页码：836-841
- 作者机构：
  
  武汉大学人民医院麻醉科湖北武汉 430060
- 作者简介：
  
  朴光宇，男，1999—，医学硕士生，主要从事肠缺血再灌注损伤与肠道菌群相互作用机制研究，E⁃mail：guangyupiao@163.com
  孟庆涛，男，1976—，医学博士，教授，主要从事围术期脏器功能保护相关机制研究，E⁃mail：mengqingtao2018@126.com
- 基金信息：
  
  国家自然科学基金资助项目(82172155;82202410)
- DOI：10.14188/j.1671-8852.2024.0674
  中图分类号： R574
- 收稿：2024-09-04，
  
  纸质出版：2025-07-25
- 稿件说明：
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朴光宇,施瑜,陈榕等.阿克曼嗜黏液菌外膜蛋白Amuc_1100调控STING信号通路减轻小鼠肠缺血再灌注损伤的机制[J].武汉大学学报（医学版）,2025,46(07):836-841.

PIAO Guangyu,SHI Yu,CHEN Rong,et al.Mechanisms of outer membrane protein Amuc_1100 of Akkermansia muciniphila attenuating intestinal ischemia⁃reperfusion injury through STING signaling pathway in mice[J].Medical Journal of Wuhan University,2025,46(07):836-841.
朴光宇,施瑜,陈榕等.阿克曼嗜黏液菌外膜蛋白Amuc_1100调控STING信号通路减轻小鼠肠缺血再灌注损伤的机制[J].武汉大学学报（医学版）,2025,46(07):836-841. DOI： 10.14188/j.1671-8852.2024.0674.

PIAO Guangyu,SHI Yu,CHEN Rong,et al.Mechanisms of outer membrane protein Amuc_1100 of Akkermansia muciniphila attenuating intestinal ischemia⁃reperfusion injury through STING signaling pathway in mice[J].Medical Journal of Wuhan University,2025,46(07):836-841. DOI： 10.14188/j.1671-8852.2024.0674.

摘要

目的

探讨阿克曼嗜黏液菌源性外膜蛋白Amuc_1100蛋白对小鼠肠缺血再灌注（IIR）损伤的影响。

方法

将18只健康雄性成年C57BL/6J小鼠随机分成3组：对照组（S组，

=6）、肠缺血再灌注组（IIR组，

=6）、肠缺血再灌注+Amuc_1100预处理组（IIR+Amuc_1100组，

=6）。对IIR+Amuc_1100组使用Amuc_1100蛋白（3 μg•d

-1

）预处理7 d，预处理完毕次日按分组制备小鼠肠缺血再灌注损伤模型，留取小肠组织。固定组织并按照标准HE染色程序染色，以观察组织改变，行Chiu's病理学损伤评分；检测小鼠肠组织超氧化物歧化酶（SOD）、丙二醛（MDA）表达水平；Western Blot法检测小鼠肠紧密连接蛋白表达以及干扰素基因刺激因子（STING）信号通路激活情况；酶联免疫吸附法检测小鼠血清中肿瘤坏死因子（TNF）⁃α、白细胞介素（IL）⁃6水平。

结果

与S组相比，IIR组Chiu's病理学损伤评分显著增高，SOD活性降低、MDA生成增加，紧密连接蛋白表达水平降低，而Amuc_1100蛋白预处理可显著降低肠缺血再灌注损伤所致Chiu's病理学损伤评分升高，增加SOD活性，并减少MDA生成，显著增加紧密连接蛋白表达水平。此外，与IIR组相比，Amuc_1100蛋白预处理显著降低IIR引起的p⁃STING、p⁃TBK1、p⁃NF⁃κB蛋白的过度表达，并显著降低IIR后的TNF⁃α、IL⁃6升高。

结论

Amuc_1100蛋白预处理能够减轻肠道肠缺血再灌注损伤，其机制可能是通过抑制STING信号通路异常激活。

Abstract

Objective

To investigate the effects of Amuc_1100

an outer membrane protein derived from

Akkermansia muciniphila

on intestinal ischemia⁃reperfusion (IIR) injury in mice.

Methods

Eighteen healthy m

ale adult C57BL/6J mice were randomly divided into three groups: Control (S group

=6)

IIR (IIR group

=6)

and IIR+Amuc_1100 pretreatment (IIR+Amuc_1100 group

=6). Mice in the IIR+Amuc_1100 group were pretreated with Amuc_1100 protein (3 µg•d

-1

) for 7 days. On the following day

IIR injury models were established by occlusion of the superior mesenteric artery for 45 min followed by 2 h reperfusion

and small intestine tissues were collected. Tissues were fixed and stained using standard HE staining procedures to observe tissue changes and perform Chiu's pathological damage score. Superoxide dismutase (SOD) and malondialdehyde (MDA) levels in the intestinal tissues were measured. Western Blot was used to detect tight junction proteins expression and stimulator of interferons genes （STING) signaling pathway activation. ELISA was used to measure serum tumor necrosis factor (TNF)⁃α and interleukin (IL)⁃6 levels.

Results

Compared with the S group

the IIR group showed a significant increase in Chiu's score

decreased SOD activity

increased MDA production

and reduced tight junction protein expression. Amuc_1100 protein pretreatment significantly reduced the increase in Chiu's pathological damage score caused by IIR

increased SOD activity

decreased MDA production

and significantly increased tight junction protein expression. Additionally

Amuc_1100 pretreatment significantly reduced the IIR⁃induced overexpression of p⁃STING

p⁃TBK1

and p⁃NF⁃κB and decreased the elevated levels of TNF⁃α and IL⁃6 induced by IIR.

Conclusion

Amuc_1100 protein pretreatment alleviates intestinal ischemia⁃reperfusion injury

possibly through the inhibition of STING signaling pathway.

关键词

Keywords

references

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