白藜芦醇对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响

查干; 方青山; 武小杰; 毛莉娜; 徐清; 黄莺; 杨硕

doi:10.14188/j.1671-8852.2024.0442

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白藜芦醇对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响

中药活性成分的作用机制研究 | 更新时间：2026-11-05

- 白藜芦醇对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响
- Effect of resveratrol on lung dendritic cells phenotype and Th2 and Th17 responses in asthmatic mice
- 武汉大学学报（医学版） 2025年46卷第4期页码：430-437
- 作者机构：
  
  武汉市第一医院呼吸与危重症医学科湖北武汉 430022
- 作者简介：
  
  查干，男，1990—，医学硕士，主治医师，主要从事慢性气道炎症研究，E‑mail:1243312238@qq.com
  杨硕，男，1979—，医学博士，主任医师，主要从事慢性气道炎症研究，E‑mail:244135490@qq.com
- 基金信息：
  
  武汉市青年项目资助(WX21Q07)
- DOI：10.14188/j.1671-8852.2024.0442
  中图分类号： R346.53
- 收稿：2024-06-12，
  
  纸质出版：2025-04-25
- 稿件说明：
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查干,方青山,武小杰等.白藜芦醇对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响[J].武汉大学学报（医学版）,2025,46(04):430-437.

ZHA Gan,FANG Qingshan,WU Xiaojie,et al.Effect of resveratrol on lung dendritic cells phenotype and Th2 and Th17 responses in asthmatic mice[J].Medical Journal of Wuhan University,2025,46(04):430-437.
查干,方青山,武小杰等.白藜芦醇对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响[J].武汉大学学报（医学版）,2025,46(04):430-437. DOI： 10.14188/j.1671-8852.2024.0442.

ZHA Gan,FANG Qingshan,WU Xiaojie,et al.Effect of resveratrol on lung dendritic cells phenotype and Th2 and Th17 responses in asthmatic mice[J].Medical Journal of Wuhan University,2025,46(04):430-437. DOI： 10.14188/j.1671-8852.2024.0442.

摘要

目的

探讨白藜芦醇（RES)对哮喘小鼠肺树突状细胞表型及Th2和Th17反应的影响。

方法

将36只C57BL/6雌性小鼠随机分为对照组、PBS/哮喘组和RES/哮喘组，每组12只。用卵清蛋白(OVA)混合液注射腹腔诱导致敏及雾化吸入激发建立C57BL/6哮喘小鼠模型。支气管肺泡灌洗液(BALF)离心后沉淀的细胞经瑞氏‑吉姆萨（Wright‑Giemsa）染色后进行分类计数，选择苏木精伊红染色(HE)和高碘酸Schiff染色(PAS)，观察小鼠支气管周围炎症和黏液分泌；采用ELISA法检测肺泡灌洗液上清液中T淋巴细胞细胞因子(IL‑4、IL‑5、IL‑13、IL‑17A)水平。流式细胞术检测肺树突状细胞数量、表面共刺激分子(CD80、CD86、CD40、MHC Ⅱ)表达、肺组织中Th1/Th2及Th17/Treg细胞百分比。定量PCR（qPCR）检测肺组织Th1（T‑bet）、Th2(GATA‑3)、Th17(RORγt)和Treg(Foxp3)等细胞相关转录因子表达水平。

结果

与PBS/哮喘组相比较，RES/哮喘组气道及肺实质炎症细胞浸润减少，气道上皮杯状细胞数量减少，黏液分泌减少，BALF中细胞总数明显减少，淋巴细胞、巨噬细胞、嗜酸粒细胞也明显减少（

0.01）；肺组织炎症病理评分及PAS

杯状细胞标准化面积(A

PAS+

/Pbm)显著低于哮喘组(

0.05)；血清总IgE、特异性IgE(OVA IgE)水平降低，BALF中IL‑4、IL‑13、IL‑5、IL‑17A水平降低；肺组织中肺树突状细胞及表面共刺激分子(CD86、CD80、MHCⅡ、CD40)、Th2、Th17及相应转录因子(GATA‑3、RORrt)的表达量显著降低。

结论

白藜芦醇可减轻哮喘小鼠气道炎症，其可能机制为通过阻止肺树突状细胞的成熟，抑制Th2和Th17反应阻止哮喘小鼠气道炎症的发生。

Abstract

Objective

To investigate the effect of resveratrol（RES） on the phenotype and Th2 and Th17 responses of lung dendritic cells in asthmatic mice.

Methods

Thirty⁃six C57BL/6 female mice were randomly divided into the control group

the PBS/asthma group

and RES/asthma group

with 12 mice in each group. The C57BL/6 mouse asthma model was established by injecting a mixture of ovalbumin (OVA) into the abdominal cavity to induce sensitization and nebulized inhalation stimulation. Cells precipitated after bronchoalveolar lavage fluid(BALF) centrifugation were stained by Wright‑Giemsa for classification count. Hematoxylin eosin staining (HE) and periodate Schiff staining (PAS) were adopted to observe inflammation and mucus secretion around the bronchi of mice. BALF was used to detect the levels of T lymphocyte cytokines

IL‑4

IL‑5

IL‑13

and IL‑17A

by using the ELISA method. Flow cytometry was used to detect the number of lung dendritic cells and the expression of surface co⁃stimulatory molecules

CD80

CD86

CD40

and MHC Ⅱ

in each group

as well as the percentage of Th1

Th2

Th17

and Treg cells in lung tissue. Quantitative PCR was used to detect the transcription factors

Th1（T‑bet）

Th2(GATA‑3)

Th17(RORγt)

Treg(Foxp3)

in lung tissue cells.

Results

Compared with the PBS/asthma group

the RES/asthma group showed reduced infiltration of infl

ammatory cells in the airway and lung parenchyma

decreased number of goblet cells in the airway epithelium

and decreased mucus secretion. The total number of cells in BALF was significantly reduced in the RES/asthma group

and lymphocytes

macrophages

and eosinophils were also significantly reduced (

0.01). The pathological score of lung tissue inflammation and the standardized area of PAS

goblet cells (A

PAS+

/Pm) were significantly lower in the asthma group (

0.05). The levels of total IgE and OVA IgE in serum decreased

and the levels of IL‑4

IL‑13

IL‑5

and IL‑17A in BALF decreased. Lung dendritic cells and the expression levels of surface co⁃stimulatory molecules (CD86

CD80

MHCⅡ

and CD40)

Th2

Th17

and corresponding transcription factors (GATA‑3 and RORrt) in lung tissue of the RES/asthma group were significantly reduced.

Conclusion

Resveratrol can alleviate airway inflammation in asthmatic mice

maybe through inhibiting dendritic cell maturation and preventing Th2 and Th17 responses.

关键词

Keywords

references

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