circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制

赵勇; 狄华明; 马建文

doi:10.14188/j.1671-8852.2024.0177

您当前的位置：

首页 >

文章列表页 >

circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制

基础医学研究 | 更新时间：2026-11-05

- circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制
- Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p
- 武汉大学学报（医学版） 2025年46卷第9期页码：1118-1123
- 作者机构：
  
  宁夏回族自治区人民医院普通外科宁夏银川 750000
- 作者简介：
  
  赵勇，男，1979—，副主任医师，主要从事胃肠道肿瘤研究,E‑mail：dihuaming2013@126.com
  马建文，男，1966—，主任医师，主要从事胃肠道肿瘤研究，E‑mail：1084294828@qq.com
- 基金信息：
  
  2022年宁夏回族自治区科技厅科技惠民项目(2022CMG03040)
- DOI：10.14188/j.1671-8852.2024.0177
  中图分类号： R735.5
- 收稿：2024-03-14，
  
  纸质出版：2025-09-26
- 稿件说明：
移动端阅览
赵勇,狄华明,马建文.circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制[J].武汉大学学报（医学版）,2025,46(09):1118-1123.

ZHAO Yong,DI Huaming,MA Jianwen.Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p[J].Medical Journal of Wuhan University,2025,46(09):1118-1123.
赵勇,狄华明,马建文.circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制[J].武汉大学学报（医学版）,2025,46(09):1118-1123. DOI： 10.14188/j.1671-8852.2024.0177.

ZHAO Yong,DI Huaming,MA Jianwen.Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p[J].Medical Journal of Wuhan University,2025,46(09):1118-1123. DOI： 10.14188/j.1671-8852.2024.0177.

摘要

目的

探讨circRHOT1以及miR‑493‑5p对结肠癌细胞生存的影响及它们之间的关系。

方法

本次实验涉及结肠癌细胞SW620以及43对结肠癌组织及癌旁组织。SW620经转染分为si‑NC组、si‑circRHOT1组、miR‑NC组、miR‑493‑5p组、si‑circRHOT1+anti‑miR‑NC组及si‑circRHOT1+anti‑miR‑493‑5p组。采用qRT‑PCR法检测circRHOT1及miR‑493‑5p RNA含量。Western Blot法检测细胞增殖及凋亡相关蛋白表达。CCK‑8以及流式细胞术分别检测SW620细胞增殖及凋亡，双萤光素酶报告实验验证circRHOT1和miR‑493‑5p靶向关系。

结果

结肠癌组织中高表达circRHOT1（

0.05），低表达miR‑493‑5p（

0.05）；敲低SW620细胞中circRHOT1的含量可以降低细胞增殖，促进细胞凋亡（

0.05）；进一步机制分析证实miR‑493‑5p是circRHOT1的功能靶标；miR‑493‑5p过表达抑制细胞增殖，加速细胞凋亡（

0.05）。miR‑493‑5p敲低可以逆转circRHOT1下调诱导的抗增殖以及促凋亡作用（

0.05）。

结论

敲低circRHOT1表达导致miR‑493‑5p高表达，从而抑制结肠癌细胞增殖及诱导细胞凋亡。

Abstract

Objective

To investigate the effects of circRHOT1 and miR‑493‑5p on the survival of colon cancer cells and the relationship between them.

Methods

In this experiment

we utilized the colon cancer cell line SW620 and 43 pairs of colon cancer tissues and adjacent non‑cancerous tissues. The SW620 cells were transfected and divided into the following groups: si‑NC group

si‑circRHOT1 group

miR‑NC group

miR‑493‑5p group

si‑circRHOT1+anti‑miR‑NC group

and si‑circRHOT1+anti‑miR‑493‑5p group. qRT‑PCR was used to measure the circRHOT1 and miR‑493‑5p expression levels. Western Blot was used to detect the expression of proteins related to cell proliferation and apoptosis. The proliferation and apoptosis of SW620 cells were assessed using the CCK‑8 assay and flow cytometry

respectively. The targeting relationship between circRHOT1 and miR‑493‑5p was validated through a dual‑luciferase reporter assay.

Results

circRHOT1 was elevated and miR‑493‑5p was reduced in colon cancer tissues (

0.05). Knocking down of circRHOT1 in SW620 cells could reduce cell proliferation and promote cell apoptosis (

0.05). Further mechanism analysis confirmed that miR‑493‑5p was the functional target of circRHOT1. Overexpression of miR‑493‑5p inhibited cell proliferation and promoted cell apoptosis (

0.05). miR‑493‑5p deletion reversed the anti‑proliferation and pro‑apoptotic effects induced by circRHOT1 downregulation in SW620 cells (

0.05).

Conclusion

Silencing circRHOT1 expression increased the miR‑493‑5p expression level in SW620 cells

thereby inhibiting proliferation and inducing apoptosis.

关键词

Keywords

references

ZHANG J , LIU H , ZHAO P , et al . Has_circ_0055625 from circRNA profile increases colon cancer cell growth by sponging miR‑106b‑5p [J ] . J Cell Biochem , 2019 , 120 ( 3 ): 3027 ‑ 3037 .

ZHOU P , XIE W , HUANG H L , et al . circRNA_100859 functions as an oncogene in colon cancer by sponging the miR‑217‑HIF‑1α pathway [J ] . Aging , 2020 , 12 ( 13 ): 1 3338‑ 13353 .

CUI W , DAI J H , MA J B , et al . circCDYL/microRNA‑105‑5p participates in modulating growth and migration of colon cancer cells [J ] . Gen Physiol Biophys , 2019 , 38 ( 6 ): 485 ‑ 495 .

TU F L , GUO X Q , WU H X , et al . Circ‑0001313/miRNA‑510‑5p/AKT2 axis promotes the development and progression of colon cancer [J ] . Am J Transl Res , 2020 , 12 ( 1 ): 281 ‑ 291 .

WANG L Y , LONG H Y , ZHENG Q H , et al . Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression [J ] . Mol Cancer , 2019 , 18 ( 1 ): 119 .

CUI F C , CHEN Y , WU X Y , et al . MicroRNA‑493‑5p suppresses colorectal cancer progression via the PI3K‑Akt‑FoxO3a signaling pathway [J ] . Eur Rev Med Pharmacol Sci , 2020 , 24 ( 8 ): 4212 ‑ 4223 .

ZHANG Q , ZHANG C , MA J X , et al . Circular RNA PIP5K1A promotes colon cancer development through inhibiting miR‑1273a [J ] . World J Gastroenterol , 2019 , 25 ( 35 ): 5300 ‑ 5309 .

PAN Z H , CAI J Y , LIN J T , et al . A novel protein encoded by circFNDC3B inhibits tumor progression and EMT through regulating Snail in colon cancer [J ] . Mol Cancer , 2020 , 19 ( 1 ): 71 .

ZHAO G , DAI G J . Hsa_circRNA_000166 promotes cell proliferation, migration and invasion by regulating miR‑330‑5p/ELK1 in colon cancer [J ] . Onco Targets Ther , 2020 , 13 : 5529 ‑ 5539 .

HE J H , HAN Z P , LUO J G , et al . Hsa_Circ_0007843 acts as a miR‑518c‑5p sponge to regulate the migration and invasion of colon cancer SW480 cells [J ] . Front Genet , 2020 , 11 : 9 .

QU S B , HAO X K , SONG W J , et al . Circular RNA circRHOT1 is upregulated and promotes cell proliferation and invasion in pancreatic cancer [J ] . Epigenomics , 2019 , 11 ( 1 ): 53 ‑ 63 .

LING S K , HE Y R , LI X X , et al . CircRHOT1 mediated cell proliferation, apoptosis and invasion of pancreatic cancer cells by sponging miR‑125a‑3p [J ] . J Cell Mol Med , 2020 , 24 ( 17 ): 9881 ‑ 9889 .

WANG J W , OUYANG X , ZHOU Z Y , et al . RNF2 promotes the progression of colon cancer by regulating ubiquitination and degradation of IRF4 [J ] . Biochim Biophys Acta Mol Cell Res , 2022 , 1869 ( 1 ): 119162 .

ZHENG Q Q , WANG B , GAO J , et al . CD155 knockdown promotes apoptosis via AKT/Bcl‑2/Bax in colon cancer cells [J ] . J Cell Mol Med , 2018 , 22 ( 1 ): 131 ‑ 140 .

LIAO X B , WEN L B , LUO L Q . The effect and mechanism of lncRNA NR2F1‑As1/miR‑493‑5p/MAP3K2 axis in the progression of gastric cancer [J ] . J Oncol , 2021 , 2021 : 3881932 .

WANG G N , FANG X S , HAN M , et al . MicroRNA‑493‑5p promotes apoptosis and suppresses proliferation and invasion in liver cancer cells by targeting VAMP2 [J ] . Int J Mol Med , 2018 , 41 ( 3 ): 1740 ‑ 1748 .

HUANG Z , WANG C Y , ZHAO X . circFIG 4 drives the carcinogenesis and metastasis of esophagus cancer via the miR‑493‑5p/E2F3 axis [J ] . Thorac Cancer , 2022 , 13 ( 6 ): 783 ‑ 794 .

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

紫草素调节Hippo信号通路对人增生性瘢痕成纤维细胞增殖和凋亡的影响

MiR⁃1184靶向胰岛素样生长因子结合蛋白4调控肝癌细胞增殖、迁移及凋亡

鼻咽低分化鳞状细胞癌细胞系中TIGD1的表达及作用

PI3K/AKT抑制剂ARQ092抑制视网膜母细胞瘤细胞的增殖、侵袭并诱导凋亡

肠道真菌群与消化系统肿瘤关系的研究进展