成纤维细胞生长因子7抑制膀胱癌细胞增殖及上皮间质转化过程

郭林发; 杨丽洁; 王晓龙; 白小杰; 刘同族

doi:10.14188/j.1671-8852.2024.0145

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成纤维细胞生长因子7抑制膀胱癌细胞增殖及上皮间质转化过程

基础医学研究 | 更新时间：2026-11-05

- 成纤维细胞生长因子7抑制膀胱癌细胞增殖及上皮间质转化过程
- Fibroblast growth factor 7 inhibits the proliferation and epithelial⁃mesenchymal transformation process of bladder cancer cells
- 武汉大学学报（医学版） 2025年46卷第5期页码：606-611
- 作者机构：
  
  武汉大学中南医院泌尿外科湖北武汉 430071
- 作者简介：
  
  郭林发，男，1997—，医学硕士生，主要从事泌尿系恶性肿瘤研究，E⁃mail:guolinfa@whu.edu.cn
  刘同族，男，1971—，医学博士，主任医师，主要从事泌尿系结石及肿瘤研究，E⁃mail:liutongzu@163.com
- 基金信息：
  
  湖北省卫生健康委员会科研项目(WJ2021M160)
- DOI：10.14188/j.1671-8852.2024.0145
  中图分类号： R737.14
- 收稿：2024-03-03，
  
  纸质出版：2025-05-25
- 稿件说明：
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郭林发,杨丽洁,王晓龙等.成纤维细胞生长因子7抑制膀胱癌细胞增殖及上皮间质转化过程[J].武汉大学学报（医学版）,2025,46(05):606-611.

GUO Linfa,YANG Lijie,WANG Xiaolong,et al.Fibroblast growth factor 7 inhibits the proliferation and epithelial⁃mesenchymal transformation process of bladder cancer cells[J].Medical Journal of Wuhan University,2025,46(05):606-611.
郭林发,杨丽洁,王晓龙等.成纤维细胞生长因子7抑制膀胱癌细胞增殖及上皮间质转化过程[J].武汉大学学报（医学版）,2025,46(05):606-611. DOI： 10.14188/j.1671-8852.2024.0145.

GUO Linfa,YANG Lijie,WANG Xiaolong,et al.Fibroblast growth factor 7 inhibits the proliferation and epithelial⁃mesenchymal transformation process of bladder cancer cells[J].Medical Journal of Wuhan University,2025,46(05):606-611. DOI： 10.14188/j.1671-8852.2024.0145.

摘要

目的

研究成纤维细胞生长因子7(FGF7)在膀胱癌中的表达情况及其对膀胱癌细胞增殖、上皮间质转化（EMT）的影响。

方法

采用免疫组化、qRT⁃PCR及Western Blot检测正常膀胱组织/细胞系和膀胱癌组织/细胞系中FGF7的表达情况。使用质粒构建过表达FGF7的膀胱癌细胞模型。采用CCK⁃8、平板克隆形成试验、划痕实验和Transwell侵袭实验检测细胞增殖、迁移和侵袭能力。采用Western Blot实验检测EMT相关蛋白表达情况。

结果

膀胱癌组织/细胞系中FGF7水平低于正常膀胱组织/上皮细胞。FGF7过表达后，膀胱癌细胞的增殖、迁移和侵袭能力受到抑制。Western Blot结果显示，FGF7过表达后，膀胱癌细胞的E⁃cadherin蛋白表达上升，N⁃cadherin、Vimentin及Snail蛋白表达下降。

结论

FGF7在膀胱癌中表达下调，过表达FGF7可以抑制膀胱癌细胞增殖及上皮间质转化过程。

Abstract

Objective

To investigate the expression of fibroblast growth factor 7 (FGF7) in bladder cancer and its influence on the proliferation and epithelial⁃mesenchymal transition (EMT) of bladder cancer cells.

Methods

The expression of FGF7 in normal bladder tissues/cell lines and bladder cancer tissues/cell lines was evaluated using immunohistochemistry

qRT⁃PCR

and Western Blot. We further generated a bladder cancer cell model with FGF7 overexpressed. Cell proliferation

migration

and invasion capabilities were assessed using CCK⁃8 assay

colony formation assay

scratch assay

and Transwell invasion assay. Western Blot analysis was employed to evaluate the expression of EMT⁃related proteins.

Results

FGF7 levels were significantly lower in bladder cancer tissues/cell lines compared to normal bladder urothelial tissues/cells. Overexpression of FGF7 inhibited the proliferation

migration

and invasion of bladder cancer cells. Besides

up⁃regulation of FGF7 could increase the expression of E⁃cadherin while decreasing the expression of N⁃cadherin

Vimentin

and Snail.

Conclusion

FGF7 is downregulated in bladder cancer

and up⁃regulation of FGF7 can inhibit the proliferation and EMT process of bladder cancer cells.

关键词

Keywords

references

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