肝脏巨噬细胞迁移抑制因子在OSA合并非酒精性脂肪肝病患者中的表达及相关性研究

孙月晨; 林泽华; 蔡伟松; 杨修平; 陈雄

doi:10.14188/j.1671-8852.2024.0043

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肝脏巨噬细胞迁移抑制因子在OSA合并非酒精性脂肪肝病患者中的表达及相关性研究

临床医学研究 | 更新时间：2026-11-05

- 肝脏巨噬细胞迁移抑制因子在OSA合并非酒精性脂肪肝病患者中的表达及相关性研究
- Expression and correlation of liver macrophage migration inhibitory factor in patients with OSA combined with NAFLD
- 武汉大学学报（医学版） 2025年46卷第3期页码：350-356
- 作者机构：
  
  武汉大学中南医院耳鼻咽喉头颈外科/睡眠医学中心湖北武汉 430071
- 作者简介：
  
  孙月晨，女，1997—，医学硕士生，主要从事睡眠呼吸障碍研究，E‑mail: yuechen.sun@whu.edu.cn
  陈雄，男，1968—，医学博士，教授，博士生导师，主要从事睡眠呼吸障碍研究，E‑mail: zn_chenxiong@whu.edu.cn
- 基金信息：
  
  国家自然科学基金资助项目(8207040451)
- DOI：10.14188/j.1671-8852.2024.0043
  中图分类号： R766
- 收稿：2024-01-17，
  
  纸质出版：2025-03-25
- 稿件说明：
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孙月晨,林泽华,蔡伟松等.肝脏巨噬细胞迁移抑制因子在OSA合并非酒精性脂肪肝病患者中的表达及相关性研究[J].武汉大学学报（医学版）,2025,46(03):350-356.

SUN Yuechen,LIN Zehua,CAI Weisong,et al.Expression and correlation of liver macrophage migration inhibitory factor in patients with OSA combined with NAFLD[J].Medical Journal of Wuhan University,2025,46(03):350-356.
孙月晨,林泽华,蔡伟松等.肝脏巨噬细胞迁移抑制因子在OSA合并非酒精性脂肪肝病患者中的表达及相关性研究[J].武汉大学学报（医学版）,2025,46(03):350-356. DOI： 10.14188/j.1671-8852.2024.0043.

SUN Yuechen,LIN Zehua,CAI Weisong,et al.Expression and correlation of liver macrophage migration inhibitory factor in patients with OSA combined with NAFLD[J].Medical Journal of Wuhan University,2025,46(03):350-356. DOI： 10.14188/j.1671-8852.2024.0043.

摘要

目的

探讨肝脏巨噬细胞迁移抑制因子(MIF)在阻塞性睡眠呼吸暂停(OSA)合并非酒精性脂肪肝病(NAFLD)患者中的表达及临床意义。

方法

选取2019年10月至2022年5月在武汉大学中南医院因代谢综合征就诊的患者50例，对其进行多导睡眠监测，根据呼吸暂停低通气指数(AHI)将其分为4组：①无OSA合并NAFLD组（对照组，7例），②轻度OSA合并NAFLD组（15例），③中度OSA合并NAFLD组（12例），④重度OSA合并NAFLD组（16例），采集血清和肝脏标本，分别应用酶联免疫吸附法和免疫组织化学染色，评估患者血清和肝脏中MIF的表达水平，分析MIF水平与OSA合并NAFLD严重程度及睡眠相关指标的相关性。

结果

与对照组相比，OSA合并NAFLD患者的血清及肝脏MIF表达水平升高，且其与OSA严重程度呈正相关。在校正体质量指数（BMI）之后，血清MIF浓度与OSA严重程度仍呈正相关（

0.05）。Logistic回归分析显示，OSA是NAFLD的独立危险因素。相关性分析显示，血清MIF水平与NAFLD严重程度呈正相关（

0.374

，P=

0.007），校正BMI之后，血清MIF水平与NAFLD严重程度仍呈正相关（

0.314，

0.028）。血清MIF水平与AHI、氧减指数（ODI）呈正相关（

0.337，

0.017；

0.292，

0.040），与LSaO

呈负相关（

r=-

0.281，

0.048）。采用偏相关

分析校正BMI后，血清MIF水平与睡眠监测指标均无相关性（

0.05）。

结论

OSA合并NAFLD疾病严重程度与MIF水平密切相关，OSA可能通过慢性间歇性缺氧刺激肝细胞释放MIF，促进NAFLD的发展。

Abstract

Objective

To explore the expression and clinical significance of liver macrophage migration inhibitory factor (MIF) in patients with obstructive sleep apnea (OSA) combined with nonalcoholic fatty liver disease (NAFLD).

Methods

Fifty patients with metabolic syndrome who were treated at Zhongnan Hospital of Wuhan University from October 2019 to May 2022 were selected

and polysomnography was performed on them. According to the apnea‑hypopnea index (AHI) index the patients were divided into four groups: ① no OSA combined with NAFLD group (7 cases)

② mild OSA combined with NAFLD group (15 cases)

③ moderate OSA combined with NAFLD group (12 cases)

and ④ severe OSA combined with NAFLD group (16 cases)

and their serum and liver specimens were collected. Enzyme‑linked immunosorbent assay and immunohistochemical staining were adopted respectively to evaluate the expression levels of MIF in the patients' serum and liver

and the relationship between MIF levels and the severity of NAFLD and sleep‑related indicators was analyzed.

Results

Compared with the control group

the patients with OSA combined with NAFLD had elevated serum and hepatic MIF expression levels

and MIF levels were positively correlated with OSA severity. After correcting for BMI

serum MIF concentration remained positively correlation with OSA severity (

0.05). Logistic regression analysis showed that OSA was an independent risk factor for NAFLD. Correlation analysis showed that serum MIF levels were positively correlated with NAFLD severity

(r=

0.374

0.007) and remained positively correlated with NAFLD severity after correction for BMI (

0.314

028). Serum MIF levels were positively correlated with AHI and ODI (

0.337

0.017;

0.292

0.040) and negatively correlated with LSaO

(

r=-

0.281

0.048). After correcting for BMI

there was no correlation between serum MIF levels and any of the sleep monitoring indicators by partial correlation analysis (

0.05).

Conclusion

The disease severity of OSA combined with NAFLD is closely related to MIF levels

and OSA may stimulate hepatocytes to release MIF through chronic intermittent hypoxia and promote the development of NAFLD.

关键词

Keywords

references

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