邻苯二甲酸酯胁迫对茭白肉质茎营养品质及其代谢响应机制的影响

陈舒妍; 张丹; 周凯; 钟兰; 李秀娟

doi:10.13300/j.cnki.hnlkxb.2026.02.024

您当前的位置：

首页 >

文章列表页 >

邻苯二甲酸酯胁迫对茭白肉质茎营养品质及其代谢响应机制的影响

食品与农业工程 | 更新时间：2026-11-05

- 邻苯二甲酸酯胁迫对茭白肉质茎营养品质及其代谢响应机制的影响
- Effects of phthalic acid esters on nutritional quality and mechanisms of metabolic response in succulent stems of Zizania latifolia
- 华中农业大学学报 2026年45卷第2期页码：228-239
- 作者机构：
  
  1.华中农业大学食品科学技术学院/环境食品学教育部重点实验室/果蔬加工与品质调控湖北省重点实验室，武汉 430070
  2.武汉市农业科学院蔬菜研究所，武汉430064
  3.湖北洪山实验室，武汉430070
- 作者简介：
  
  陈舒妍，E-mail：chensy9527@foxmail.com
  李秀娟，E-mail：lixiujuan@mail.hzau.edu.cn
- 基金信息：
  
  湖北省农业科技创新中心(2021-620-000-001-31)
- DOI：10.13300/j.cnki.hnlkxb.2026.02.024
  中图分类号： TS207
- 收稿：2025-04-29，
  
  纸质出版：2026-03-15
- 稿件说明：
移动端阅览
陈舒妍，张丹，周凯，等.邻苯二甲酸酯胁迫对茭白肉质茎营养品质及其代谢响应机制的影响［J］.华中农业大学学报，2026，45（2）：228‐239.

CHEN Shuyan,ZHANG Dan,ZHOU Kai,et al.Effects of phthalic acid esters on nutritional quality and mechanisms of metabolic response in succulent stems of Zizania latifolia[J].Journal of Huazhong Agricultural University,2026,45(02):228-239.
陈舒妍，张丹，周凯，等.邻苯二甲酸酯胁迫对茭白肉质茎营养品质及其代谢响应机制的影响［J］.华中农业大学学报，2026，45（2）：228‐239. DOI： 10.13300/j.cnki.hnlkxb.2026.02.024.

CHEN Shuyan,ZHANG Dan,ZHOU Kai,et al.Effects of phthalic acid esters on nutritional quality and mechanisms of metabolic response in succulent stems of Zizania latifolia[J].Journal of Huazhong Agricultural University,2026,45(02):228-239. DOI： 10.13300/j.cnki.hnlkxb.2026.02.024.

摘要

为探究邻苯二甲酸酯类化合物（phthalic acid esters，PAEs）胁迫下茭白肉质茎营养品质的变化机制，采用超高效液相色谱串联质谱结合非靶向代谢组学技术对不同质量分数PAEs处理下茭白肉质茎代谢机制进行系统解析。结果显示，高质量分数PAEs显著增加肉质茎粗纤维（46.15%）、可溶性蛋白（410%）和游离氨基酸（22.96%）含量，但降低白度、维生素C（50.11%）及可溶性固形物含量；中质量分数PAEs提高肉质茎总灰分（25.00%）和可溶性固形物含量（64.99%），而低质量分数PAEs下肉质茎粗纤维与还原糖含量最低。代谢组学鉴定差异代谢物689个。中质量分数PAEs诱导了227种代谢物变化，其中氨基酸类下调、脂类上调，显著影响了精氨酸和脯氨酸代谢通路；高质量分数PAEs引发267种代谢物变化，导致氨基酸类和苯丙素类上调，核苷酸类和有机酸下调。

Abstract

The mechanisms of metabolic response in the succulent stems of

Zizania latifolia

under different concentrations of phthalic acid esters（PAEs） were systematically analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry combined with untargeted metabolomics to study the mechanism underlying changes in nutritional quality of

Zizania latifolia

succulent stems under phthalate esters（PAEs） stress. The results showed that high concentrations of PAEs significantly increased the content of crude fiber（46.15%）， soluble protein（410%）， and free amino acids（22.96%） in the succulent stems， but decreased the whiteness， the content of vitamin C （50.11%）， and the content of soluble solids. Medium concentrations of PAEs increased the content of total ash（16.67%） and water-soluble ash（11.48%） in the succulent stems， while the content of crude fiber and reducing sugars in the succulent stems were the lowest under low concentrations of PAEs. 689 differential metabolites were identified with untargeted metabolomics. Medium concentrations of PAEs induced changes in 227 metabolites， among which amino acids were down-regulated and lipids were up-regulated， significantly affecting the metabolic pathways of arginine and proline. High concentrations of PAEs triggered changes in 267 metabolites， leading to the up-regulation of amino acids and phenylpropanoids， and the down-regulation of nucleotides and organic acids. It will help to clarify the effects of PAEs pollution on the nutritional quality of crops， and provide a scientific basis for agricultural production and environmental protection to promote the

sustainable development of agriculture and ensure food security.

关键词

Keywords

references

LI X X，WANG Q，JIANG N，et al.Occurrence，source，ecological risk，and mitigation of phthalates （PAEs） in agricultural soils and the environment：a review［J/OL］.Environmental research，2023，220：115196［2025-04-29］.https：//doi.org/10.1016/j.envres.2022.115196.

XU Y L，SUN Y Q，LEI M，et al.Phthalates contamination in sediments：a review of sources，influencing factors，benthic toxicity，and removal strategies［J/OL］.Environmental pollution，2024，344：123389［2025-04-29］.https：//doi.org/10.1016/j.envpol.2024.123389.

LI Y，YAN H Q，LIU Q Y，et al.Accumulation and transport patterns of six phthalic acid esters （PAEs） in two leafy vegetables under hydroponic conditions［J/OL］.Chemosphere，2020，249：126457［2025-04-29］.https：//doi.org/10.1016/j.chemosphere.2020.126457.

YANG J T，WANG J F，LIAO X Y，et al.Chain modeling for the biogeochemical nexus of cadmium in soil-rice-human health system［J/OL］.Environment international，2022，167：107424［2025-04-29］.https：//doi.org/10.1016/j.envint.2022.107424.

陈学斌，赵海明，莫测辉，等.接种邻苯二甲酸酯（PAEs）降解菌缓解PAEs对玉米胁迫效应和机制研究［J］.生态环境学报，2017，26（3）：493-499.CHEN X B，ZHAO H M，MO C H，et al. Alleviation of phthalic acid ester（PAE） stress on maize by inoculating PAEs-degrading strain［J］.Ecology and environmental sciences，2017，26（3）：493-499 （in Chinese with English abstract） .

PATEL M K，KUMAR M，LI W Q，et al.Enhancing salt tolerance of plants：from metabolic reprogramming to exogenous chemical treatments and molecular approaches［J/OL］.Cells，2020，9（11）：2492［2025-04-29］.https：//doi.org/10.3390/cells9112492.

LI X F，ZHANG Y，WANG J X，et al.Revealing the metabolomics and biometrics underlying phytotoxicity mechanisms for polystyrene nanoplastics and dibutyl phthalate in dandelion （ Taraxacum officinale ）［J/OL］.Science of the total environment，2023，905：167071［2025-04-29］.https：//doi.org/10.1016/j.scitotenv.2022.167071.

WANG J，CHEN G，CHRISTIE P，et al.Occurrence and risk assessment of phthalate esters （PAEs） in vegetables and soils of suburban plastic film greenhouses［J］.Science of the total environment，2015，523：129-137.

SUN Y Y，CHEN J，ZHU L Z.Phthalate esters decreased nutritional value of rice grains via redirecting glycolytic carbon flow from grain quality formation toward antioxidative defense［J/OL］.Journal of hazardous materials，2025，488：137358［2025-04-29］.https：//doi.org/10.1016/j.jhazmat.2024.137358.

ZHANG D，ZHOU K，LIU C Q，et al.Dissipation，uptake，translocation and accumula tion of five phthalic acid esters in sediment- Zizania latifolia system［J/OL］.Chemosphere，2023，315：137651［2025-04-29］.https：//doi.org/10.1016/j.chemosphere.2023.137651.

WANG L J，LIU M M，TAO W D，et al.Pollution characteristics and health risk assessment of phthalate esters in urban soil in the typical semi-arid city of Xi’an，Northwest China［J］.Chemosphere，2018，191：467-476.

CHENG Z P，YAO Y M，SUN H W.Comparative uptake，translocation and subcellular distribution of phthalate esters and their primary monoester metabolites in Chinese cabbage （ Brassica rapa var.Chinensis）［J/OL］.Science of the total environment，2020，742：140550［2025-04-29］.https：//doi.org/10.1016/j.scitotenv.2020.140550.

SINGH V，GUIZANI N，AL-ZAKWANI I，et al.Sensory texture of date fruits as a function of physicochemical properties and its use in date classification［J］.Acta alimentaria，2015，44（1）：119-125.

戚姝姝，刘晓晨.邻苯二甲酸二丁酯和邻苯二甲酸单丁酯对菠菜生长的毒性效应及累积特征数据集［J］.农业大数据学报，2025，7（1）：132-140.QI S S，LIU X C.Toxic effects and cumulative characteristics of dibutyl phthalate and monobutyl phthalate on spinach datasets［J］.Journal of agricultural big data，2025，7（1）：132-140 （in Chinese with English abstract） .

侯伶俐，杨雄榜，董雪妮，等.逆境胁迫对苦荞花期总黄酮含量及关键酶基因表达的影响［J］.核农学报，2016，30（1）：184-192.HOU L L，YANG X B，DONG X N，et al.Effect of environmental stresses on the contents of total fl avonoids and corresponding gene expression in Fagopyrum tataricum during florescence［J］.Journal of nuclear agricultural sciences，2016，30（1）：184-192 （in Chinese with English abstract） .

DI MARTINO C，DELFINE S，PIZZUTO R，et al.Free amino acids and glycine betaine in leaf osmoregulation of spinach responding to increasing salt stress［J］.New phytologist，2003，158（3）：455-463.

LIANG L J，LI W B，TIAN M，et al.Metabolomic profiling of five hulless barley （ Hordeum vulgare L.） with different［J］.Genetic resources and crop evolution，2022，69（5）：1843-1853.

FOUGÈRE F，LE RUDULIER D，STREETER J G.Effects of salt stress on amino acid，organic acid，and carbohydrate composition of roots，bacteroids，and cytosol of alfalfa（ Medicago sativa L.）［J］.Plant physiology，1991，96（4）：1228-1236.

HOCHBERG U，DEGU A，CRAMER G R，et al.Cultivar specific metabolic changes in grapevines berry skins in relation to deficit irrigation and hydraulic behavior［J］.Plant physiology and biochemistry，2015，88：42-52.

QUAN X Y，QIAN Q F，YE Z L，et al.Metabolic analysis of two contrasting wild barley genotypes grown hydroponically reveals adaptive strategies in response to low nitrogen stress［J］.Journal of plant physiology，2016，206：59-67.

DIAZ C，PURDY S，CHRIST A，et al.Characterization of markers to determine the extent and variability of leaf senescence in Arabidopsis a metabolic profiling approach［J］.Plant physiology，2005，138（2）：898-908.

CHEYNIER V，COMTE G，DAVIES K M，et al.Plant phenolics：recent advances on their biosynthesis，genetics，and ecophysiology［J］.Plant physiology and biochemistry，2013，72：1-20.

GILL S S，ANJUM N A，HASANUZZAMAN M，et al.Glutathione and glutathione reductase：a boon in disguise for plant abiotic stress defense operations［J］.Plant physiology and biochemistry，2013，70：204-212.

NIKOLANTONAKI M，JULIEN P，COELHO C，et al.Impact of glutathione on wines oxidative stability：a combined sensory and metabolomic study［J/OL］.Frontiers in chemistry，2018，6：182［2025-04-29］.https：//doi.org/10.3389/fchem.2018.00182.

RUELLAND E，VALENTOVA O.Editorial：lipid signaling in plant development and responses to environmental stresses［J/OL］.Frontiers in plant science，2016，7：324［2025-04-29］.https：//doi.org/10.3389/fpls.2016.00324.

UPCHURCH R G.Fatty acid unsaturation，mobilization，and regulation in the response of plants to stress［J］.Biotechnology letters，2008，30（6）：967-977.

ZHANG H L，DU W C，PERALTA-VIDEA J R，et al.Metabolomics reveals how cucumber （ Cucumis sativus ） reprograms metabolites to cope with silver ions and silver nanoparticle-induced oxidative stress［J］.Environmental science & technology，2018，52（14）：8016-8026.

MA B，HE Y，CHEN H H，et al.Dissipation of polycyclic aromatic hydrocarbons （PAHs） in the rhizosphere：synthesis through meta-analysis［J］.Environmental pollution，2010，158（3）：855-861.

HANSON A D，BEAUDOIN G A，MCCARTY D R，et al.Does abiotic stress cause functional B vitamin deficiency in plants［J］.Plant physiology，2016，172（4）：2082-2097.

ZHAO L Q，ZHANG Y W，WANG L，et al.Stereoselective metabolomic and lipidomic responses of lettuce （ Lactuca sativa L.） exposing to chiral triazole fungicide tebuconazole［J/OL］.Food chemistry，2022，371：131209［2025-04-29］. https：//doi.org/10.1016/j.foodchem.2021.131209.

SHARMA A，SHAHZAD B，REHMAN A，et al.Response of phenylpropanoid pathway and the role of polyphenols in plants under abiotic stress［J/OL］.Molecules，2019，24（13）：2452［2025-04-29］.https：//doi.org/10.3390/molecules24132452.

CHATTOPADHAYAY M K，TIWARI B S，CHATTOPADHYAY G，et al.Protective role of exogenous polyamines on salinity-stressed rice （ Oryza sativa ） plants［J］.Physiologia plantarum，2002，116（2）：192-199.

ROUSSOS P A，PONTIKIS C A.Changes of free，soluble conjugated and bound polyamine titers of jojoba explants under sodium chloride salinity in vitro ［J］.Journal of plant physiology，2007，164（7）：895-903.

NOCTOR G，MHAMDI A，CHAOUCH S，et al.Glutathione in plants：an integrated overview［J］.Plant，cell & environment，2012，35（2）：454-484.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

茭白对重金属污染沉积物的修复作用及其生理响应

湖北不同品种黄精的化学组成及其体外功能活性比较

茭白压缩力学特性试验研究

播期对黑稻品质与产量的影响

复合菌剂对猪粪水重金属的钝化效果及沙地燕麦的吸收特征