1.广东生态工程职业学院植物有害生物防控与生物环境健康应用技术协同创新中心,广州 510520
2.广东湛江红树林国家级自然保护区管理局,湛江 524088
王宏洪,E-mail:angongong2003@163.com
陶冶,E-mail:taoye126@163.com
收稿:2025-09-30,
纸质出版:2026-03-15
移动端阅览
王宏洪,陈廷丰,张灿,等.广东省红树林中华新锥线虫的形态和分子鉴定[J].华中农业大学学报,2026,45(2):190‐198.
WANG Honghong,CHEN Tingfeng,ZHANG Can,et al.Morphological and molecular identification of awl nematode, Neodolichodorus sinensis associated with mangrove forest in Guangdong Province, China[J].Journal of Huazhong Agricultural University,2026,45(02):190-198.
王宏洪,陈廷丰,张灿,等.广东省红树林中华新锥线虫的形态和分子鉴定[J].华中农业大学学报,2026,45(2):190‐198. DOI: 10.13300/j.cnki.hnlkxb.2026.02.020.
WANG Honghong,CHEN Tingfeng,ZHANG Can,et al.Morphological and molecular identification of awl nematode, Neodolichodorus sinensis associated with mangrove forest in Guangdong Province, China[J].Journal of Huazhong Agricultural University,2026,45(02):190-198. DOI: 10.13300/j.cnki.hnlkxb.2026.02.020.
2021-2022年,分别在广东湛江红树林国家级自然保护区和广东镇海湾红树林国家湿地公园采集到2个新锥线虫种群样本。为探明该线虫的系统发育关系,利用形态学、分子生物学和系统发育的方法,将其鉴定为中华新锥线虫(
Neodolichodorus sinensis
),并获得中华新锥线虫湛江种群和镇海湾种群的18S rDNA和28S rDNA D2D3区序列数据。系统发育树构建结果显示:基于18S rDNA序列的系统发育树中中华新锥线虫与波斯湾新锥线虫(
N. persiangulfus
)聚为高度支持的单系分支;基于28S rDNA D2D3区序列的系统发育树中中华新锥线虫与波斯湾新锥线虫和白龙尾新锥线虫(
N. bachlongviensis
)聚为高度支持的单系分支。以上结果表明,中华新锥线虫与波斯湾新锥线虫互为姐妹种。
During 2021-2022, two awl nematode populations were collected from the Guangdong Zhanjiang Mangrove National Nature Reserve and the Guangdong Zhenhai Bay Mangrove National Wetland Park, respectively.To investigate the phylogenetic relationships among closely related species of
Neodolichodorus
, morphological, molecular and phyloge
netic analyses were conducted.These analyses confirmed that both populations were identified as
Neodolichodorus sinensis
.The 18S rDNA and 28S rDNA D2D3 region sequences were obtained for the Zhanjiang and Zhenhai Bay populations of
N.sinensis
, and phylogenetic trees were constructed based on these sequences.The results showed that in the phylogenetic tree based on 18S rDNA sequences,
N. sinensis
formed a highly supported monophyletic clade with
N. persiangulfus
.In the phylogenetic tree based on the 28S rDNA D2D3 region sequences,
N. sinensis
clustered with
N. persiangulfus
and
N.bachlongviensis
in a highly supported monophyletic clade.This study clarifies the relationships between
N. sinensis
and its closely related species.This paper identifies
N. sinensis
and
N. persiangulfus
as sister cryptic species.
ANDRÁSSY I.Evolution as a basis for the systematization of nematodes[M].London:Pitman,1976.
GHARAHKHANI A,POURJAM E,PEDRAM M.Occurrence of Neodolichodorus persiangulfus n.sp.(Nematoda:Dolichodoridae) in mangrove forests of southern Iran[J/OL].Forest pathology,2019,49(6):e12563[2025-09-30].https://doi.org/10.1111/efp.12563.
TRINH Q P,NGUYEN H T,LE T M L,et al.Morphological and molecular characterizations of Neodolichodorus bachlongviensis sp.n.(Nematoda:Dolichodoridae),a new plant-parasitic nematode species from Bach Long Vy island in Vietnam[J].Australasian plant pathology,2024,53(2):141-150.
SIDDIQI M R. Tylenchida parasites of plants and insects[M]. 2nd edition. Wallingford, UK: CABI Publishing, 2000.
VOVLAS N, TROCCOLI A, LAMBERTI F. An illustrated diagnostic compendium o f Neodolichodorus Andrassy, 1976 (Nematoda: Dolichodoridae), with additional data on morphology of N. citri s Jacob & Loof, 1996[J]. Journal of Nematode Morphology and Systematics, 2004, 6(2): 129-138.
HODDA M,NAMBIAR L. Neodolichodorus australis n.sp.(Nematoda:Dolichodoridae) on carrot in Australia[J].Australasian plant pathology,2005,34(1):1-9.
GERAERT E. The Dolichodoridae of the world: identification of the family Dolichodoridae (Nematoda) [M]. Ghent, Belgium: Academia Press, 2011.
ZHUO K,WANG H H,LIAO J L. Neodolichodorus sinensis sp.nov.(Nematoda:Dolichodoridae) from China[J].Zootaxa,2010,2362(1):63-68.
XU C L,XIE H,LI Y.Description of Neodolichodorus hainanensis n.sp.(Nematoda:Dolichodoridae) from rhizosphere soil of golf turf in China[J].Zootaxa,2012,3236(1):: 62-68.
中国科学院中国植物志编辑委员会.中国植物志(第58卷)[M].北京:科学出版社,1979:33.Editorial Committee of Flora of China.Flora of China (Volume 58) [M].Beijing: Science Press, 1979: 33 (in Chinese) .
中国科学院中国植物志编辑委员会.中国植物志(第70卷)[M].北京: 科学出版社, 2002: 45.Editorial Committee of Flora of China.Flora of China (Volume 58) [M].Beijing: Science Press, 2002:45 (in Chinese) .
冯志新.植物线虫学[M].北京:中国农业出版社,2001.FENG Z X.Plant Nematology[M].Beijing:China Agriculture Press,2001 (in Chinese) .
WANG H H,ZHUO K,CAI R D,et al.Morphological and molecular characterisation of Paralongidorus sacchari (Nematoda:Longidoridae),a new record of needle nematode in China[J].European journal of plant pathology,2019,155(2):615-625.
DE MAN J G.Die,frei in der reinen Erde und im süssen Wasser lebenden Nematoden der niederländischen Fauna:eine systematisch-faunistische Monographie [M].Leiden:E.J.Brill,1884.
MUNDO-OCAMPO M,TROCCOLI A,SUBBOTIN S A,et al.Synonymy of Afenestrata with Heterodera supported by phylogenetics with molecular and morphological characterisation of H. koreana comb.n.and H.Orientalis comb.n.(Tylenchida:Heteroderidae)[J].Nematology,2008,10(5):611-632.
DE LEY P,FELIX M A,FRISSE L,et al.Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda:Cephalobidae)[J].Nematology,1999,1(6):591-612.
HOLTERMAN M,VAN DER WURFF A,VAN DEN ELSEN S,et al.Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades[J].Molecular biology and evolution,2006,23(9):1792-1800.
缪绅裕,陈桂珠,陈正桃,等.广东湛江保护区红树林种群的生物量及其分布格局[J].广西植物,1998,18(1):11-23.MIAO S Y,CHEN G Z,CHEN Z T,et al.Biomasses and distributive patterns of mangrove populations in Zhanjiang Nature Reserves,Guangdong,China[J].Guihaia,1998,18(1):11-23 (in Chinese with English abstract) .
华国栋,庄礼凤,李家祥,等.广东台山镇海湾红树林国家湿地公园土壤有机碳含量及其影响因素分析[J].林业与环境科学,2021,37(6):118-123.HUA G D,ZHUANG L F,LI J X,et al.Contents of soil organic carbon of mangrove in Haiwan mangrove national wetland park,Taishan Town,Guangdong and their influencing factors[J].Forestry and environmental science,2021,37(6):118-123 (in Chinese with English abstract) .
蒋学建,罗基同,秦元丽,等.我国红树林有害生物研究综述[J].广西林业科学,2006,35(2):66-69.JIANG X J,LUO J T,QIN Y L,et al.A summary of mangrove pests in China[J].Guangxi forestry science,2006,35(2):66-69 (in Chinese) .
徐家雄,林明生,陈瑞屏,等.粤港地区红树林害虫种类调查[J].广东林业科技,2008,24(2):46-49.XU J X,LIN M S,CHEN R P,et al.Investigation of pest insects on mangrove in Guangdong and Hongkong area[J].Guangdong forestry science and technology,2008,24(2):46-49 (in Chinese with English abstract) .
付小勇,秦长生,赵丹阳.中国红树林湿地昆虫群落及害虫研究进展[J].广东林业科技,2012(4):56-61.FU X Y,QIN C S,ZHAO D Y.Research progress in insect community and pests of Chinese mangrove[J].Guangdong forestry science and technology,2012(4):56-61 (in Chinese with English abstract) .
范航清,刘文爱,曹庆先.广西红树林害虫生物生态特性与综合防治技术研究[M].北京:科学出版社,2012.FAN H Q,LIU W A,CAO Q X.Study on biological and ecological characteristics and integrated control techniques of mangrove pests in Guangxi[M].Beijing:Science Press,2012 (in Chinese) .
李志刚,戴建青,叶静文,等.中国红树林生态系统主要害虫种类、防控现状及成灾原因[J].昆虫学报,2012,55(9):1109-1118.LI Z G,DAI J Q,YE J W,et al.Species,control status and outbreak causes of main pest insects in mangrove ecosystems in China[J].Acta entomologica sinica,2012,55(9):1109-1118 (in Chinese with English abstract) .
纪燕玲,蔡选光,纪丹虹,等.粤东地区红树林主要害虫种类调查及危险性评价[J].中国森林病虫,2015,34(5):20-24.JI Y L,CAI X G,JI D H,et al.Investigation and risk assessment of main pests on mangrove in eastern region of Guangdong[J].Forest pest and disease,2015,34(5):20-24 (in Chinese with English abstract) .
杨华,凌斯全,田龙艳,等.广东省红树林有害生物调查与防治建议[J].湿地科学,2025,23(2):262-273.YANG H,LING S Q,TIAN L Y,et al.Current status of mangrove pests in Guangdong Province and suggestions for their control management[J].Wetland science,2025,23(2):262-273 (in Chinese with English abstract) .
王宏洪.深圳福田红树林线虫群体多样性研究[D].广州:华南农业大学,2010.WANG H H.Population diversity of nematodes in Futian mangrove forest,Shenzhen[D].Guangzhou:South China Agricultural University,2010 (in Chinese with English abstract) .
NICHOLAS W L,STEWART A.Criconemella a vicenniae N.sp.(Nematoda:Criconematidae) and enchodel us coomansi N.sp.(Nematoda:Nordiidae) associated with the roots of the mangrove Avicennia marina (Forsk.) VIerh[J].Nematologica,1984,30(4):429-436.
HODDA M,NICHOLAS W L.Meiofauna associated with mangroves in the Hunter River estuary and Fullerton Cove,south-eastern Australia[J].Australian journal of marine and freshwater research,1985,36(1):41-50.
SIDDIQI M R, BOOTH W. Meloidogyne (Hypsoperine) mersa sp.n.(Nematoda; Tylenchina) attacking Sonneratia alba trees in mangrove forest in Brunei Darussalam[J].Afro-Asian Journal of Nematology, 1991, 1: 212-220.
VAN DEN BERG E,QUÉNÉHERVÉ P. Hirschmanniella caribbeana sp.n.and new records of Pratylenchus spp.(Pratylenchidae:Nematoda) from Guadeloupe,French West Indies[J].Nematology,2000,2(2):179-190.
0
浏览量
2
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621