黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析

唐子瑞; 周旭; 江欣欣; 李张萍; 张桂蓉; 马徐发; 姬伟; 魏开建

doi:10.13300/j.cnki.hnlkxb.2026.01.024

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黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析

动物科学·动物医学 | 更新时间：2026-11-05

- 黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析
- Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri
- 华中农业大学学报 2026年45卷第1期页码：266-277
- 作者机构：
  
  1.华中农业大学水产学院/农业农村部淡水生物繁育重点实验室，武汉430070
  2.浙江海洋大学海洋科学与技术学院，舟山 316022
- 作者简介：
  
  唐子瑞，E-mail： tzr@webmail.hzau.edu.cn
  魏开建，E-mail： kjwei@mail.hzau.edu.cn
- 基金信息：
  
  国家自然科学基金项目(31772851);湖北省农业农村厅渔业资源监测项目(CJ2023155-10)
- DOI：10.13300/j.cnki.hnlkxb.2026.01.024
  中图分类号： Q959.4
- 收稿：2025-06-01，
  
  纸质出版：2026-01-15
- 稿件说明：
移动端阅览
唐子瑞，周旭，江欣欣，等.黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析［J］.华中农业大学学报，2026，45（1）：266‐277.

TANG Zirui,ZHOU Xu,JIANG Xinxin,et al.Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri[J].Journal of Huazhong Agricultural University,2026,45(01):266-277.
唐子瑞，周旭，江欣欣，等.黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析［J］.华中农业大学学报，2026，45（1）：266‐277. DOI： 10.13300/j.cnki.hnlkxb.2026.01.024.

TANG Zirui,ZHOU Xu,JIANG Xinxin,et al.Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri[J].Journal of Huazhong Agricultural University,2026,45(01):266-277. DOI： 10.13300/j.cnki.hnlkxb.2026.01.024.

摘要

在哺乳动物中，白细胞介素-17受体（interleukin-17 receptor，IL-17R）家族通过介导IL-17细胞因子的信号来调控炎症反应。为探究

IL⁃17R

基因在鱼类对病原体感染的免疫应答中的潜在功能，从黄颡鱼（

Pelteobagrus fulvidraco，Pf

）中克隆了5个

Pf_IL⁃117R

基因（

Pf_IL⁃117RA

至

Pf_IL⁃17RE

）的部分cDNA序列，并对5个基因的序列分子特征、组织表达谱以及鲇爱德华氏菌感染和免疫刺激物刺激后的表达变化进行分析。结果显示，5个

Pf_IL⁃17R

基因的开放阅读框长度依次为2 430（

Pf_IL⁃17RA

）、1 590（

Pf_IL⁃17RB

）、2 106（

Pf_IL⁃17RC

）、2 235（

Pf_IL⁃17RD

）和2 307 bp（

Pf_IL⁃17RE

），分别编码809、529、701、744和768个氨基酸。氨基酸序列分析显示，黄颡鱼5个

Pf_IL⁃17R

基因与其他硬骨鱼类的同源基因序列相似性较高，其中与斑点叉尾鮰同源基因序列相似性最高。实时荧光定量PCR结果显示，5个

Pf_IL⁃17R

基因在健康黄颡鱼组织中呈现不同的mRNA表达谱，但它们在血液、性腺和鳃中的表达量较高。黄颡鱼感染鲇爱德华氏菌后，鳃、皮肤和头肾中5个

Pf_IL⁃17R

基因mRNA表达水平显著上调，而脾脏中

Pf_IL⁃17RB

、

Pf_IL⁃17RC

、

Pf_IL⁃17RD

和

Pf_IL⁃17RE

基因mRNA表达量显著下调。此外，脂多糖、植物血凝素和聚肌胞苷酸刺激可诱导黄颡鱼外周血白细胞中4个

Pf_IL⁃17R

基因（

Pf_IL⁃17RC

除外）mRNA的表达。研究表明，5个

Pf_IL⁃17R

基因可能在黄颡鱼对病原体感染的免疫应答中发挥重要作用。

Abstract

The interleukin-17 receptor （IL-17R） family in mammals regulates inflammatory responses by mediating the signaling of the IL-17 cytokine. Partial cDNA sequences of five

IL⁃17R

genes （

IL⁃17RA

IL⁃17RE

） were cloned from yellow catfish （

Pelteobagrus fulvidraco

，

） to study the potential role of

IL⁃17R

gene in the immu

ne response to pathogenic infection in fish. The molecular characteristics of the sequence，expression profiles of tissue，and changes in expression of five genes in yellow catfish infected with

Edwardsiella ictaluri

and stimulated with immunostimulants were analyzed. The results showed that the open reading frame （ORF） of the five

IL⁃17R

genes including

IL⁃17RA，Pf

IL⁃17RB，Pf

IL⁃17RC，Pf

IL⁃17RD，Pf

IL⁃17RE

was

2 430 bp，1 590 bp，2 106 bp，2 235 bp，and 2 307 bp，encoding 809 amino acids （aa），529 aa，701 aa，744 aa，and 768 aa，respectively. The results of analyzing the sequence of aa showed that the five

IL⁃17R

genes in yellow catfish had high sequence similarity with homologous genes of other teleosts，among which the sequence similarity with homologous genes of channel catfish was the highest. The results of analyses with Real time fluorescence quantitative PCR showed that the five

IL⁃17R

genes exhibited different profiles of mRNA in healthy yellow catfish，but the levels of their expression were higher in the blood，gonads，and gills. The levels of mRNA expression of five

Pf⁃IL⁃17R

genes were significantly upregulated in the gills，skin，and head kidney of yellow catfish infected with

Edwardsiella ictaluri

，while the expression levels of

IL⁃17RB

，

Pf_IL⁃17RC

，

IL⁃17RD

and

IL⁃17RE

mRNAs were significantly down-regulated in the spleen （

0.05 or

0.01）. Moreover，the levels of mRNA expression of four

IL⁃17R

genes （with the exception of

IL⁃17RC

） were induced in the isolated peripheral blood leukocytes （PBLs） of yellow

catfish stimulated with lipopolysaccharide （LPS），phytohemagglutinin （PHA） and polyinosinic-polycytidylic acid （Poly I：C）. It is indicated that the five

IL⁃17R

genes may play important roles in the immune response of yellow catfish to pathogen infection.

关键词

Keywords

references

SONG X Y，QIAN Y C.IL-17 family cytokines mediated signaling in the pathogenesis of inflammatory diseases［J］.Cellular signalling，2013，25（12）：2335-2347.

GAFFEN S L.Structure and signalling in the IL-17 receptor family［J］.Nature reviews immunology，2009，9（8）：556-567.

SU Y，HUANG J L，ZHAO X H，et al.Interleukin-17 receptor D constitutes an alternative receptor for interleukin-17A important in psoriasis-like skin inflammation［J/OL］.Science immunology，2019，4（36）：eaau9657［2025-06-01］. https：//doi.org/10.1126/sciimmunol.aau9657.

KRAMER J M，HANEL W，SHEN F，et al.Cutting edge：identification of a pre-ligand assembly domain （PLAD） and ligand binding site in the IL-17 receptor［J］.The journal of immunology，2007，179（10）：6379-6383.

HAUDENSCHILD D，MOSELEY T，ROSE L，et al.Soluble and transmembrane isoforms of novel interleukin-17 receptor-like protein by RNA splicing and expression in prostate cancer［J］.Journal of biological chemistry，2002，277（6）：4309-4316.

WU B J，GONG J，LIU L L，et al.Evolution of prokaryotic homologues of the eukaryotic SEFIR protein domain［J］.Gene，2012，492（1）：160-166.

NOVATCHKOVA M，LEIBBRANDT A，WERZOWA J，et al.The STIR-domain superfamily in signal transduction，development and immunity［J］.Trends in biochemical sciences，2003，28（5）：226-229.

WRIGHT J F，BENNETT F，LI B L，et al.The human IL-17F/IL-17A heterodimeric cytokine signals through the IL-17RA/IL-17RC receptor complex［J］.The journal of immunology，2008，181（4）：2799-2805.

LIANG S C，TAN X-Y，LUXENBERG D P，et al.Interleukin （IL）-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides［J］.The journal of experimental medicine，2006，203（10）：2271-2279.

SHEN F，RUDDY M J，PLAMONDON P，et al.Cytokines link osteoblasts and inflammation：microarray analysis of interleukin-17- and TNF -α-induced genes in bone cells［J］.Journal of leukocyte biology，2004，77（3）：388-399.

WANG X Q，LI C，THONGDA W，et al.Characterization and mucosal responses of interleukin 17 family ligand and receptor genes in channel catfish Ictalurus punctatus ［J］.Fish & shellfish immunology，2014，38（1）：47-55.

DING Y，AI C X，MU Y N，et al.Molecular characterization and evolution analysis of five interleukin-17 receptor genes in large yellow croaker Larimichthys crocea ［J］.Fish & shellfish immunology，2016，58：332-339.

XUE T，LIU Y P，CAO M，et al.Genome-wide identification of interleukin-17 （IL-17）/interleukin-17 receptor （IL-17R） in turbot （ Scophthalmus maximus ） and expression pattern analysis after Vibrio anguillarum infection［J/OL］.Developmental & comparative immunology，2021，121：104070［2025-06-01］. https：//doi.org/10.1016/j.dci.2021.104070.

HARADA N，OKAMURA Y，KONO T，et al.Identification of two interleukin 17 receptor C （IL-17RC） genes and their binding activities to three IL-17A/F ligands in the Japanese medaka， Oryzias latipes ［J/OL］.Developmental & comparative immunology，2021，124：104179［2025-06-01］. https：//doi.org/10.1016/j.dci.2021.104179.

ZHOU X，ZHANG G R，JI W，et al.Expression and function analysis of interleukin-17A/F1，2，and 3 genes in yellow catfish （ Pelteobagrus fulvidraco ）：distinct bioactivity of recombinant IL-17A/F1，2，and 3［J/OL］.Frontiers in immunology，20 21，12：626895［2025-06-01］. https：//doi.org/10.3389/fimmu.2021.626895.

ZHOU X，JIANG X X，ZHANG G R，et al.Molecular characterization，expression and function analysis of interleukin-17B，C and D genes in yellow catfish （ Pelteobagrus fulvidraco ）［J/OL］.Aquaculture，2022，552：737962［2025-06-01］. https：//doi.org/10.1016/j.aquaculture.2022.737962.

MAO X B，TIAN Y，WEN H S，et al.Effects of Vibrio harveyi infection on serum biochemical parameters and expression profiles of interleukin-17 （IL-17）/interleukin-17 receptor （IL-17R） genes in spotted sea bass［J/OL］.Developmental & comparative immunology，2020，110：103731［2025-06-01］. https：//doi.org/10.1016/j.dci.2020.103731.

ISHIGAME H，KAKUTA S，NAGAI T，et al.Differential roles of interleukin-17A and-17F in host defense against mucoepithelial bacterial infection and allergic responses［J］.Immunity，2009，30（1）：108-119.

SONG X Y，ZHU S，SHIPQ，et al.IL-17RE is the functional receptor for IL-17C and mediates mucosal immunity to infection with intestinal pathogens［J］.Nature immunology，2011，12（12）：1151-1158.

MCALLISTER F，HENRY A，KREINDLER J L，et al.Role of IL-17A，IL-17F，and the IL-17 receptor in regulating growth-related oncogene-α and granulocyte colony-stimulating factor in bronchial epithelium：implications for airway inflammation in cystic fibrosis［J］.The journal of immunology，2005，175（1）：404-412.

IWAKURA Y，ISHIGAME H，SAIJO S，et al.Functional specialization of interleukin-17 family members［J］.Immunity，2011，34（2）：149-162.

SHI Y G，ULLRICH S J，ZHANG J，et al.A novel cytokine receptor-ligand pair［J］.Journal of biological chemistry，2000，275（25）：19167-19176.

YANG R B，NG C K D，WASSERMAN S M，et al.A novel interleukin-17 receptor-like protein identified in human umbilical vein endothelial cells antagonizes basic fibroblast growth factor-induced signaling［J］.Journal of biological chemistry，2003，278（35）：33232-33238.

KOLLS J K，LINDÉN A.Interleukin-17 family members and inflammation［J］.Immunity，2004，21（4）：467-476.

MONTE M M，WANG T H，HOLLAND J W，et al.Cloning and characterization of rainbow trout interleukin-17A/F2 （IL-17A/F2） and IL-17 receptor A：expression during infection and bioactivity of recombinant IL-17A/F2［J］.Infection and immunity，2013，81（1）：340-353.

RICKEL E A，SIEGEL L A，YOON B P，et al.Identification of functional roles for both IL-17RB and IL-17RA in mediating IL-25-induced activities［J］.The journal of immunology，2008，181（6）：4299-4310.

KUESTNER R E，TAFT D W，HARAN A，et al.Identification of the IL-17 receptor related molecule IL-17RC as the receptor for IL-17F［J］.The journal of immunology，2007，179（8）：5462-5473.

FORTIER M E，KENT S，ASHDOWN H，et al.The viral mimic，polyinosinic：polycytidylic acid，induces fever in rats via an interleukin-1-dependent mechanism［J］.American journal of physiology-regulatory，integrative and comparative physiology，2004，287（4）：R759-R766.

LU Y C，YEH W C，OHASHIPS.LPS/TLR4 signal transduction pathway［J］.Cytokine，2008，42（2）：145-151.

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