华中农业大学资源与环境学院,武汉 430070
高青蕊,E-mail: 1647943792@qq.com
蔡红梅,E-mail: caihongmei@mail.hzau.edu.cn
收稿:2024-11-19,
纸质出版:2025-09-15
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高青蕊,侯星宇,蔡红梅.长江中下游主栽水稻耐低温及铁锰毒害品种的筛选与鉴定[J].华中农业大学学报,2025,44(5):36‐49.
GAO Qingrui,HOU Xingyu,CAI Hongmei.Screening and identifying rice varieties with tolerance of low temperature and toxicity of iron and manganese in middle and lower reaches of Yangtze River[J].Journal of Huazhong Agricultural University,2025,44(05):36-49.
高青蕊,侯星宇,蔡红梅.长江中下游主栽水稻耐低温及铁锰毒害品种的筛选与鉴定[J].华中农业大学学报,2025,44(5):36‐49. DOI: 10.13300/j.cnki.hnlkxb.2025.05.004.
GAO Qingrui,HOU Xingyu,CAI Hongmei.Screening and identifying rice varieties with tolerance of low temperature and toxicity of iron and manganese in middle and lower reaches of Yangtze River[J].Journal of Huazhong Agricultural University,2025,44(05):36-49. DOI: 10.13300/j.cnki.hnlkxb.2025.05.004.
为筛选长江中下游冷浸田适应性品种,以该地区57份主栽水稻品种为试验材料,通过营养液培养试验进行低温及铁锰毒害筛选;以筛选获得的耐感品种为研究对象,分析两者在生长表型、根系构型、铁锰含量及抗氧化酶活性上的差异。结果显示,在对照条件下,无论是耐冷还是耐铁锰毒害品种,大多数生长指标和生理生化指标相较于敏感品种均没有显著差异;但在处理条件下,耐冷和耐铁锰毒害品种的生长指标和生理生化指标均优于敏感品种。在低温处理下,耐冷品种的存活率、地上部生物量、叶绿素b含量及POD活性均显著高于敏感品种,地上部MDA含量显著低于敏感品种。在++Fe
2+
处理下,耐亚铁毒害品种的株高、总根长、根系体积和表面积、根尖数、地上部干质量均显著高于亚铁毒害敏感品种,耐亚铁毒害品种根中铁含量显著低于亚铁毒害敏感品种,根和地上部SOD的活性及地上部POD的活性均显著高于亚铁毒害敏感品种。在++Mn
2+
处理下,耐锰毒害品种的株高、主根长、根系表面积、根和地上部干质量均显著高于锰毒害敏感品种,耐锰毒害品种根和地上部的锰含量显著低于锰毒害敏感品种,地上部SOD和POD的活性均显著高于锰毒害敏感品种。通过维恩图分析和复合逆境筛选获得了荃优丝苗(5#)、红香优丝苗(9#)、嘉丰优2号(19#)、全两优534(33#)、玮两优534(34#)、华两优2817(53#)等高产多抗品种,可作为冷浸田推广种植的候选品种。
57 main rice varieties planted in the middle and lower reaches of the Yangtze River were used to conduct hydroponic culture experiment under the low temperature and the toxicity of iron(Fe
2+
) and manganese(Mn
2+
) to screen the adaptive rice varieties in cold-waterlogged paddy fields in this area.The selected tolerant and sensitive varieties were used to analyze the differences in the growth phenotype,root architecture,content of iron and manganese,and the activity of antioxidant enzyme.The results showed that there were no significant differences in most growth indexes and physiological and biochemical indexes in cold tolerant or toxicity of iron and manganese tolerant varieties compared to that in sensitive varieties under control conditions.The growth indexes and physiological and biochemical indexes in cold tolerant or toxicity of iron and manganese tolerant varieties were better than those in sensitive v
arieties under treatment.The survival rate,aboveground biomass,content of chlorophyll b,and activity of POD in cold tolerant varieties was significantly higher than that in cold sensitive varieties under low temperature treatment,while the content of MDA aboveground in cold tolerant varieties was significantly lower than that in cold sensitive varieties.The plant height,total root length,root volume and surface area,root tip number,and aboveground dry weight of Fe
2+
tolerant varieties was significantly higher than that of ++Fe
2+
sensitive varieties under ++Fe
2+
treatment.The content of iron in the root of ++Fe
2+
tolerant varieties was significantly lower than that of ++Fe
2+
sensitive varieties,and the activity of SOD and POD in the root and aboveground parts of ++Fe
2+
tolerant varieties was significantly higher than that of ++Fe
2+
sensitive varieties.The plant height,primary root length,root surface area,root and shoot dry weight in Mn
2+
tolerant varieties were higher than that in ++Mn
2+
sensitive varieties under ++Mn
2+
treatment.The content of Mn
2+
in root and shoot of ++Mn
2+
tolerant varieties was significantly lower than that of ++Mn
2+
sensitive varieties,while the activity of SOD and POD in the shoot of ++Mn
2+
tolerant varieties was higher than that of ++Mn
2+
sensitive varieties.Several varieties such as Quanyousimiao(5#),Hongxiangyousimiao(9#),Jiafengyou No.2(19#),Quanliangyou No. 534(33#),Weiliangyou No. 534(34#)and Hualiangyou No. 2817(53#)with high-yield and multi-resistance were obtained through Venn diagram analysis and composite stress screening,which can be used as candidate varieties for popularizing and planting in cold-waterlogged paddy fields.
严如玉,甘国渝,赵希梅,等.我国水稻优势产区生产格局及施肥现状研究[J].中国稻米,2023,29(3):1-8.YAN R Y,GAN G Y,ZHAO X M,et al.Study on the production pattern and fertilization status of rice dominant production areas in China[J].China rice,2023,29(3):1-8 (in Chinese with English abstract) .
梁玉刚,李静怡,周晶,等.中国水稻栽培技术的演变与展望[J].作物研究,2022,36(2):180-188.LIANG Y G,LI J Y,ZHOU J,et al.Evolution and prospect of rice cultivation technology in China[J].Crop research,2022,36(2):180-188 (in Chinese with English abstract) .
赵凌,赵春芳,周丽慧,等.中国水稻生产现状与发展趋势[J].江苏农业科学,2015,43(10):105-107.ZHAO L,ZHAO C F,ZHOU L H,et al.Current situation and development trend of rice production in China[J].Jiangsu agricultural sciences,2015,43(10):105-107 (in Chinese) .
全国土壤普查办公室.中国土壤[M].北京:中国农业出版社,1998.National Soil Census Office.China soil[M].Beijing:China Agriculture Press,1998 (in Chinese) .
李庆奎.中国水稻土[M].北京:科学出版社,1992.LI Q K. Chinese paddy soil[M].Beijing:Science Press,1992 (in Chinese) .
张嘉伟.水稻芽期耐低温种质资源及抗寒剂筛选[D].长沙:湖南农业大学,2022.ZHANG J W.Selection of low temperature resistant germplasm resources at bud stage and cold resistance agents of rice [D].Changsha:Hunan Agricultural University,2022 (in Chinese with English abstract) .
蔡志欢.水稻苗期低温种质资源筛选及低温调控[D].长沙:湖南农业大学,2018.CAI Z H.Screening and low temperature regulation of low temperature germplasm resources in rice seedling stage[D].Changsha:Hunan Agricultural University,2018 (in Chinese with English abstract) .
熊英,欧阳杰,何永歆,等.芽期耐低温淹水的水稻种质的评价与筛选[J].杂交水稻,2015,30(4):54-58.XIONG Y,OUYANG J,HE Y X,et al.Evaluation and screening of rice genetic resources with tolerance to low temperature and submergence in germinating stage [J].Hybrid rice,2015,30(4):54-58 (in Chinese with English abstract) .
潘晓雪,胡明瑜,王忠伟,等.不同水稻种质资源重要农艺性状与发芽期耐寒性鉴定研究[J].作物杂志,2021(1):47-53.PAN X X,HU M Y,WANG Z W,et al.Evaluation of agronomic traits and cold tolerance at germination stage in rice( Oryza sativa L.) germplasms[J].Crops,2021(1):47-53 (in Chinese with English abstract) .
蔡慧姝,喻毅涛,刘鹏,等.水稻耐铁毒基因型筛选及边缘细胞对铁毒的反应特性[J].广东微量元素科学,2007,11:16-22.CAI H S,YU Y T,LIU P,et al.Screening for iron toxicity tolerant rice genotype and response of root border cells to excessive Fe 2+ [J].Guangdong trace elements science,2007,11:16-22 (in Chinese with English abstract) .
赵燕,项华,熊娜,等.亚铁胁迫对水稻生长及矿质元素积累的影响[J].中国稻米,2018,24(3):30-38.ZHAO Y,XIANG H,XIONG N,et al.Effects of ferrous toxicity on growth of rice and accumulation of some mineral elements in rice plants[J].China rice,2018,24(3):30-38 (in Chinese with English abstract) .
BRESOLIN A P S,DOS SANTOS R S,WOLTER R C D,et al.Iron tolerance in rice:an efficient method for performing quick early genotype screening[J/OL].BMC research notes,2019,12(1):361[2024-11-19].https://doi.org/10.1186/s13104-019-4362-5.
YOSHIDA S,FORNO D A,COCK J H,et al.Laboratory manual for physiological studies of rice [M].Manila:International Rice Research Institute,1976.
鲍士旦.土壤农化分析 [M].北京:中国农业出版社,2000.BAO S.Soil agricultural chemistry analysis [M].Beijing:China Agricultural Press,2000 (in Chinese) .
王学奎,黄见良.植物生理生化实验原理与技术[M].北京:高等教育出版社,2015.WANG X K,HUANG J L.Principles and techniques of plant physiology and biochemistry experiments [M].Beijing:Higher Education Press,2015 (in Chinese) .
SHAEMA P,SHARMA N,DESWAL R.The molecular biology of the low-temperature response in plants [J].Bioassays,2005,27(10):1048-1059.
KIM S I,ANDAYA V C,TAI T H.Cold sensitivity in rice ( Oryza sativa L.) is strongly correlated with a naturally occurring I99V mutation in the multifunctional glutathione transferase isoenzyme GSTZ2 [J].Biochemical journal,2011,435(2):373-380.
潘孝武,李小湘,黎用朝,等.湖南早籼稻品种芽期和苗期耐冷性初步研究[J].杂交水稻,2015,30(5):59-64.PAN X W,LI X X,LI Y C,et al.Preliminary studies on cold tolerance at germination stage and seedling stage of early Indica rice in Hunan [J].Hybrid rice,2015,30(5):59-64 (in Chinese with English abstract) .
李海林,殷绪明,龙小军.低温胁迫对水稻幼苗抗寒性生理生化指标的影响[J].安徽农学通报,2006,10(11):50-53.LI H L,YIN X M,LONG X J.Effects of chilling stress on physiological and biochemical indexes of chill-resistance in rice seedlings [J].Anhui agricultural science bulletin,2006,10(11):50-53 (in Chinese with English abstract) .
孙擎,杨再强,高丽娜,等.低温对早稻幼穗分化期叶片生理特性的影响及其与产量的关系[J].中国生态农业学报,2014,22(11):1326-1333.SUN Q,YANG Z Q,GAO L N,et al.Effect of low temperature stress on physiological characteristics of flag leaf and its relationship with grain yield during panicle primordium differentiation stage of early rice[J].Chinese journal of eco-agriculture,2014,22(11):1326-1333 (in Chinese with English abstract) .
赵杨,魏颖娟,邹应斌.低温胁迫下早稻幼苗叶片和根系的生理指标变化及其品种间差异[J].核农学报,2015,29(4):792-798.ZHAO Y,WEI Y J,ZOU Y B.Physiological index variation and its cultivar differences of seedling leaves and roots of early cropping rice under lower temperature stress [J].Journal of nuclear agricultural sciences,2015,29(4):792-798 (in Chinese with English abstract) .
任红茹.孕穗期低温冷害对水稻产量形成、生理特性和稻米品质的影响[D].扬州:扬州大学,2018.REN H R.Effects of low-temperature cold damage at booting stage on yield formation,physiological characteristics and rice quality of rice [D].Yangzhou:Yangzhou University,2018 (in Chinese with English abstract) .
傅泰露.水稻不同生育期的耐冷性及鉴定指标的筛选[D].成都:四川农业大学,2008.FU T L.The cold tolerance and screening of cold tolerance identification indexes in rice varieties during different stages [D].Chengdu:Sichuan Agriculture University,2008 (in Chinese with English abstract) .
SATVIR K,MEENAKSHI A,ANIL K G,et al.Exploration of biochemical and molecular diversity in chickpea seeds to categorize cold stress-tolerant and susceptible genotypes [J].Acta physiology plant,2012,34(2):569-580.
王树刚,王振林,王平等.不同小麦品种对低温胁迫的反应及抗冻性评价 [J].生态学报,2011,31(4):1064-1072.WANG S G,WANG Z L,WANG P,et al.Evaluation of wheat freezing resistance based on the responses of the physiological indices to low temperature stress [J].Acta ecologica sinica,2011,31(4):1064-1072 (in Chinese with English abstract) .
彭新湘,山内稔.植物对铁毒的抗性机理[J].植物生理学通讯,1996,32(6):465-469.PENG X X,YAMAUCHI M.The resistant mechanisms for plants to iron toxicity [J].Plant physiology communications,1996,32(6):465-469 (in Chinese) .
STEIN R J,DUARTE G L,SCHEUNEMANN L,et al.Genotype variation in rice ( Oryza sativa L.) tolerance to Fe toxicity might be linked to root cell wall lignification[J/OL].Frontiers in plant science,2019,10:746[2024-11-19].https://doi.org/10.3389/fpls.2019.00746.
SHENG H,ZENG J,LIU Y,et al.Sulfur mediated alleviation of Mn toxicity in polish wheat relates to regulating Mn allocation and improving antioxidant system [J/OL].Frontier in plant science,2016,7:1382[2024-11-19].https://doi.org/10.3389/fpls.2016.01382.
ONAGA G,DRANE K N,ISMAIL A M.Understanding the regulation of iron nutrition:can it contribute to improving iron toxicity in rice ?[J] Functional plant biology,2016,43:709-726.
SHAO J,YAMAJI N,SHEN R F, et al.The key to Mn homeostasis in plants:regulation of Mn transporters[J].Trends in plant science,2017,22(3):215-224.
FECHT-CHRISTOFFERS M M,BRAUN H P,LEMAITRE-GUILLIER C,et al.Effect of manganese toxicity on the proteome of the leaf apoplast in cowpea [J].Plant physiology,2003,133:1935-1946.
RIBERA-FONSECA A,INOSTROZA-BLANCHETEAU C,CARTES P,et al.Early induction of Fe-SOD gene expression is involved in tolerance to Mn toxicity in perennial ryegrass [J].Plant physiology and biochemistry,2013,73:77-82.
王思潮,曹凑贵,李成芳,等.鄂东南冷浸田不同中稻品种产量及生理研究[J].湖北农业科学,2014,53(16):3736-3740.WANG S C,CAO C G,LI C F,et al.Yield and physiology of middle-season rice in cold-waterlogged paddy field at the Southeastern of Hubei Province [J].Hubei agricultural sciences,2014,53(16):3736-3740 (in Chinese with English abstract) .
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