基于功能化金纳米颗粒的表面增强拉曼光谱检测Ag

白向茹; 谭金龙; 罗伟; 周文宇; 郭心灵

doi:10.13300/j.cnki.hnlkxb.2025.04.031

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基于功能化金纳米颗粒的表面增强拉曼光谱检测Ag

技术与方法 | 更新时间：2026-11-05

- 基于功能化金纳米颗粒的表面增强拉曼光谱检测Ag
- Accurate and interference-free SERS detection of Ag⁺ based on functionalized gold nanoparticles
- 华中农业大学学报 2025年44卷第4期页码：318-324
- 作者机构：
  
  信阳农林学院药学院，信阳 464000
- 作者简介：
  
  白向茹，E-mail：baimaster@126.com
  郭心灵，E-mail：Gxl15637681099@126.com
- 基金信息：
  
  国家自然科学基金项目(21874102);河南省科技攻关项目(232102320294;242102320266;252102310515;252102311246);河南省青年骨干教师培养计划(2024GGJS154);信阳农林学院青年基金项目(QN2022021);信阳农林学院国家级科研项目培育基金项目(pyjj20230101)
- DOI：10.13300/j.cnki.hnlkxb.2025.04.031
  中图分类号： X832
- 收稿：2024-03-07，
  
  纸质出版：2025-07-05
- 稿件说明：
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白向茹，谭金龙，罗伟，等.基于功能化金纳米颗粒的表面增强拉曼光谱检测Ag⁺［J］.华中农业大学学报，2025，44（4）：318‐324. DOI: 10.13300/j.cnki.hnlkxb.2025.04.031.

BAI Xiangru,TAN Jinlong,LUO Wei,et al.Accurate and interference-free SERS detection of Ag⁺ based on functionalized gold nanoparticles[J].Journal of Huazhong Agricultural University,2025,44(04):318-324. DOI: 10.13300/j.cnki.hnlkxb.2025.04.031.
白向茹，谭金龙，罗伟，等.基于功能化金纳米颗粒的表面增强拉曼光谱检测Ag⁺［J］.华中农业大学学报，2025，44（4）：318‐324. DOI: 10.13300/j.cnki.hnlkxb.2025.04.031. DOI：

BAI Xiangru,TAN Jinlong,LUO Wei,et al.Accurate and interference-free SERS detection of Ag⁺ based on functionalized gold nanoparticles[J].Journal of Huazhong Agricultural University,2025,44(04):318-324. DOI: 10.13300/j.cnki.hnlkxb.2025.04.031. DOI：

摘要

为实现准确、无干扰检测Ag

，建立一种基于功能化金纳米颗粒的表面增强拉曼光谱（surface enhanced Raman scattering，SERS）传感检测方法。通过形成硫-金键，将对巯基苯甲腈（MBN）修饰在金纳米颗粒表面，作为功能化金纳米颗粒；基于配位作用，Ag

可与功能化金纳米颗粒表面MBN的氰基发生反应，导致相邻金纳米颗粒聚集，产生“热点”效应，使MBN在生物“静默区”2 228 cm

-1

处的SERS信号增强；SERS信号增强强度随Ag

浓度增加而增大，由此建立Ag

的SERS传感检测方法。结果显示：构建的SERS传感检测Ag

方法，在0.1~100 nmol/L区间内线性关系良好，对Ag

的最低可检出浓度为0.1 nmol/L，远低于美国保护署规定饮用水中Ag

浓度应小于0.93 μmol/L的限定要求。Ag

实际水样加标回收检测结果显示，回收率为99.7%~101.4%。研究结果表明，建立的Ag

的SERS传感检测方法操作简单、耗时较短、特异性强，可应用于痕量Ag

的快速检测。

Abstract

The silver ion（Ag

） poses serious threats on the environment and the health for human. Therefore， the accurate and interference-free detection of Ag

is of great scientific significance with practical value of application. The surface-enhanced Raman spectroscopy（SERS） sensing detection technology based on functionalized gold nanoparticles was established to realize the accurate and interference free detection of Ag

. When Ag

was present， adjacent gold nanoparticles were connec

ted and underwent controlled aggregation to form a chain-like structure based on the coordination effect between Ag

and cyanogroup（-CN） in merhydryl benzonitrile（MBN） modified on the surface of gold nanoparticles， generating a “hotspot” effect. The SERS signal of MBN at 2 228 cm

-1

in the biological “silent zone” was enhanced， and the degree of enhancement increased with the increase of the concentration of Ag

. The results showed that the SERS sensing technology established for detecting Ag

had a good linear relationship in the concentration interval of 0.1 nmol/L and 100 nmol/L. The minimum detectable concentration of Ag

was 0.1 nmol/L， much lower than the limit requirement of the US Environmental Protection Agency that the concentration of Ag

in drinking water should be less than 0.93 μmol/L. The detection with Ag

spiked recovery was performed in actual water samples， with a recovery rate ranged from 99.7% to 101.4%.

关键词

Keywords

references

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