双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留

郑美杰; 郑腾飞; 刘春华; 王明月; 李秀娟

doi:10.13300/j.cnki.hnlkxb.2025.04.030

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双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留

技术与方法 | 更新时间：2026-11-05

- 双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留
- Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS
- 华中农业大学学报 2025年44卷第4期页码：310-317
- 作者机构：
  
  1.华中农业大学食品科学技术学院，武汉 430070
  2.中国热带农业科学院分析测试中心，海口 570000
- 作者简介：
  
  郑美杰，E-mail：mjzheng2021@163.com
  王明月，E-mail：hkwmy0815@163.com
  李秀娟，E-mail：lixiujuan@mail.hzau.edu.cn
- 基金信息：
  
  海南省科技厅重点研发计划项目(ZDYF2021XDNY304);湖北省农业科技创新中心项目(2021-620-000-001-31)
- DOI：10.13300/j.cnki.hnlkxb.2025.04.030
  中图分类号： O657.7
- 收稿：2024-05-20，
  
  纸质出版：2025-07-05
- 稿件说明：
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郑美杰，郑腾飞，刘春华，等.双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留［J］.华中农业大学学报，2025，44（4）：310‐317.

ZHENG Meijie,ZHENG Tengfei,LIU Chunhua,et al.Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS[J].Journal of Huazhong Agricultural University,2025,44(04):310-317.
郑美杰，郑腾飞，刘春华，等.双棉签棒固相微萃取结合UPLC-MS/MS快速检测香蕉中农药残留［J］.华中农业大学学报，2025，44（4）：310‐317. DOI： 10.13300/j.cnki.hnlkxb.2025.04.030.

ZHENG Meijie,ZHENG Tengfei,LIU Chunhua,et al.Rapid detection of 11 common pesticide residues in bananas with double-swab solid-phase microextraction combined with UPLC-MS/MS[J].Journal of Huazhong Agricultural University,2025,44(04):310-317. DOI： 10.13300/j.cnki.hnlkxb.2025.04.030.

摘要

为简化香蕉中多农药残留检测步骤，缩短检测时长，降低分析检测成本，采用固相微萃取技术结合超高效液相色谱-串联质谱建立香蕉中11种常见农药残留的快速准确检测方法。以棉签棒作为支撑载体制备2种广谱性大容量萃取头，以双萃取头模式对稀释后的香蕉匀浆样品中农药进行萃取，以微量甲醇解吸萃取头上农药后再利用超高效液相色谱-串联质谱检测，采用基质外标法定量。结果显示，11种农药在香蕉基质中的检出限为0.23~2.43 μg/kg，定量限为0.68~7.36 μg/kg；不同加标水平下回收率为86.2%~109.8%，相对标准偏差为0.6%~11.2%，符合农药残留检测相关规定。研究结果表明，新建方法简单、快速、灵敏、准确、成本低，能满足香蕉中多农药残留的快速检测要求。

Abstract

A rapid and accurate method for detecting 11 common pesticide residues in bananas was established with solid-phase microextraction combined with ultra-high performance liquid chromatography tandem mass spectrometry （SPME-UPLC-MS/MS） to simplify the steps of detecting multi-pesticide residues in bananas， shorten the time of detection， and reduce the cost of detection. Swabs were used as support carriers to prepare the double extraction heads with broad-spectrum and large-capacity. The pesticides were extracted from diluted banana homogenate samples with the double extraction heads. Trace amounts of methanol were used to desorb pesticides from the heads， followed by detection with SPME-UPLC-MS/MS and quantification with method of matrix-matched external standard. The results showed that the limit of detection of 11 pesticides in bananas was 0.23-2.43 μg/kg， with the limit of quantification of 0.68-7.36 μg/kg. The rate of recovery at different spiked levels was 86.2%-109.8%， with a relative standard deviation of 0.6%-11.2%， which complies with the relevant regulations of detecting pesticide residues. It is indicated that the established method is simple， rapid， sensitive， accurate， and cost-low， and can meet the requirements for rapid detection of multi-pesticide residues in bananas.

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references

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