华南农业大学植物保护学院/绿色农药全国重点实验室/天然农药与化学生物学教育部重点实验室 ,广州 510642
马露露,E-mail:2065905621@qq.com
江定心,E-mail:dxj2005@scau.edu.cn
收稿:2024-03-08,
纸质出版:2025-07-05
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马露露,江定心.白纹伊蚊四氟甲醚菊酯抗性品系室内筛选及抗性遗传分析[J].华中农业大学学报,2025,44(4):174‐180.
MA Lulu,JIANG Dingxin.Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance[J].Journal of Huazhong Agricultural University,2025,44(04):174-180.
马露露,江定心.白纹伊蚊四氟甲醚菊酯抗性品系室内筛选及抗性遗传分析[J].华中农业大学学报,2025,44(4):174‐180. DOI: 10.13300/j.cnki.hnlkxb.2025.04.017.
MA Lulu,JIANG Dingxin.Laboratory screening of dimefluthrin-resistant strains of Aedes albopictus and analysis of hereditary modes of resistance[J].Journal of Huazhong Agricultural University,2025,44(04):174-180. DOI: 10.13300/j.cnki.hnlkxb.2025.04.017.
为明确四氟甲醚菊酯抗性白纹伊蚊(
Aedes albopictus
)品系的抗药性水平,选择四氟甲醚菊酯抗性品系,进行杂交和回交试验,确定四氟甲醚菊酯抗性白纹伊蚊种群的显性度(
D
)、细胞质影响因子和遗传基因。结果显示,FRS种群的抗性基因频率与抗性品系白纹伊蚊接近、FSR种群的敏感性强;FRS种群和FSR种群之间不交叠,分属2个不同的区间,2个杂交种群之间差异显著;4组回交后代BC1(FSR♀×SS♂)、BC2(FRS♀×SS♂)、BC3(FSR♀×RR♂)以及BC4(FRS♀×RR♂)的实际与期望剂量对数-死亡率(LD-P)曲线之间有差别,实际卡方值(χ
2
)明显大于期望卡方值。研究结果表明,白纹伊蚊对四氟甲醚菊酯的抗性遗传为多基因控制的母系遗传,这种遗传方式将使白纹伊蚊抗性种群迅速发展。在使用四氟甲醚菊酯防治白纹伊蚊时,为达到好的杀虫效果,且尽量避免害虫产生抗药性,就需要注意可能会带来不利影响的多个因素,如用药剂量、杀虫剂使用方法以及用药周期等,不同蚊虫对不同药剂产生的抗药性的遗传方式可能不同,因此在研制药剂以及用药时也要注意。
To clarify the resistance level to dimefluthrin in
Aedes albopictus
and to select a strain that is resistant to dimefluthrin,the mechanisms and genetics of the resistance should be studied. Cross and backcross tests were conducted to caculate the 95% confidence interval for the degree of dominance in the two hybrid populations. Additionally,the actual and expected curves of the four groups of backcross offspring were observed,and the actual and expected chi-square values of the four groups of backcross offspring were calculated to determine the degree of dominance (
D
),identify cytoplasmic influencing factors,and elucidate the genes associated with the heredity of the dimefluthrin-resistant population of
A. albopictus.
The results indicated that the resistance frequency in the FRS population is close to that of the resistant strain (RR),while the sensitivity of the FSR population is notebly high. The FRS and FSR populations did not overlap,indicating a significant difference between the FRS and FSR hybrids. Furthermore,the actual response of the LD-P line for BC1 (FSR♀×SS♂),BC2 (FRS♀×SS♂),BC3 (FSR♀×RR♂) and BC4 (FRS♀×RR♂) differed from the expected response of the LD-P line,with the actual Chi-square value (χ
2
) being significa
ntly greater than the expected Chi-square value. Therefore,the inheritance of resistance to dimefluthrin in
A. albopictus
is polygenic and exhibits maternal effect,which can lead to the rapid development of resistant populations. When using dimefluthrin to control
A. albopictus
,it is essential to consider several factors that may negatively impact insecticidal efficacy and contribute to the development of resistance. These factors include dosage,application methods and the timing of insecticide use. Different mosquitoes species may have different hereditary modes of resistance to different insecticides,therefore,it is crucial to monitor the development and application of insecticides carefully.
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