4类食用菌中RNA病毒的检测和种类分析

王鑫; 刘莹莹; 肖俊博; 李华平; 李鹏飞

doi:10.13300/j.cnki.hnlkxb.2025.04.016

您当前的位置：

首页 >

文章列表页 >

4类食用菌中RNA病毒的检测和种类分析

植物保护 | 更新时间：2026-11-05

- 4类食用菌中RNA病毒的检测和种类分析
- Detection and species analysis of RNA mycoviruses in four types of edible mushrooms
- 华中农业大学学报 2025年44卷第4期页码：160-173
- 作者机构：
  
  绿色农药全国重点实验室/华南农业大学植物保护学院，广州510642
- 作者简介：
  
  王鑫，E-mail：1163592165@qq.com
  李鹏飞，E-mail：lipengfei@scau.edu.cn
- 基金信息：
  
  国家香蕉产业技术体系建设专项(CARS-31-09)
- DOI：10.13300/j.cnki.hnlkxb.2025.04.016
  中图分类号： S432.4
- 收稿：2024-11-25，
  
  纸质出版：2025-07-05
- 稿件说明：
移动端阅览
王鑫，刘莹莹，肖俊博，等.4类食用菌中RNA病毒的检测和种类分析［J］.华中农业大学学报，2025，44（4）：160‐173.

WANG Xin,LIU Yingying,XIAO Junbo,et al.Detection and species analysis of RNA mycoviruses in four types of edible mushrooms[J].Journal of Huazhong Agricultural University,2025,44(04):160-173.
王鑫，刘莹莹，肖俊博，等.4类食用菌中RNA病毒的检测和种类分析［J］.华中农业大学学报，2025，44（4）：160‐173. DOI： 10.13300/j.cnki.hnlkxb.2025.04.016.

WANG Xin,LIU Yingying,XIAO Junbo,et al.Detection and species analysis of RNA mycoviruses in four types of edible mushrooms[J].Journal of Huazhong Agricultural University,2025,44(04):160-173. DOI： 10.13300/j.cnki.hnlkxb.2025.04.016.

摘要

为明确生产中不同食用菌中的病毒种类，采用高通量测序技术，对53株侧耳属（

Pleurotus

spp.）和10株灵芝属（

Ganoderma

spp.），以及17株金针菇（

Flammulina filiformis

）和12株鸡腿菇（

Coprinus comatus

）的菌株中的真菌病毒进行检测，对新发现的可能新病毒进行PCR扩增和序列分析，在序列比较的基础上进行系统发育树分析。BLASTx序列比对分析结果显示

，

4类92个食用菌菌株共有51条contigs可注释为41种病毒，RT-PCR扩增和序列分析共鉴定到13种可能的新病毒，其中，侧耳属菌株中发现7种，鸡腿菇中发现3种，金针菇中发现1种，灵芝属菌株中发现2种。系统发育分析结果显示，这些病毒分属于+ssRNA病毒、-ssRNA病毒和dsRNA病毒，分布在8个病毒科，包括

Barnaviridae、Botourmiaviridae、Deltaflexiviridae、Mymonaviridae、Partitiviridae、Phenuiviridae、Phlegiviridae

和1个未分类的

Bunyavirales

病毒。研究结果表明

：

4类食用菌中存在较为丰富的真菌病毒。

Abstract

To clarify the types of viruses present in various edible mushrooms during production， high-throughput sequencing technology was employed to detect fungal viruses in 53 strains of

Pleurotus

spp.， 10 strains of

Ganoderma

spp.， 17 strains of

Flammulina filiformis

， and 12 strains of

Coprinus comatus

.PCR amplification and sequence analysis were performed on newly identified potential vi

ruses， and phylogenetic tree analyses were conducted based on sequence comparisons.BLASTx sequence alignment results indicated that a total of 51 contigs were annotated as 41 viruses detected from 92 strains of four types of edible mushrooms.Thirteen potential novel viruses were identified through RT-PCR amplification and sequence analysis， including seven species found in

Pleurotus

spp.， three species in

Coprinus comatus

， one species in

Flammulina filiformis

， and two species in

Ganoderma

spp..Phylogenetic analyses showed that these viruses belong to +ssRNA viruses， -ssRNA viruses， and dsRNA viruses， distributed across wight viral families：

Barnaviridae

，

Botourmiaviridae

，

Deltaflexiviridae

，

Mymonaviridae

，

Partitiviridae

，

Phenuiviridae， Phlegiviridae

， and an unclassified member of the order

Bunyavirales

.In conclusion， there is a relatively abundant presence of fungal viruses in the four types of edible mushrooms.

关键词

Keywords

references

BELL V，SILVA C G，GUINA J，et al.Mushrooms as future generation healthy foods［J/OL］.Frontiers in nutrition，2022，9：1050099［2024-11-25］.https：//doi.org/10.3389/fnut.2022.1050099.

中国食用菌协会.2021年度全国食用菌统计调查结果分析［J］.中国食用菌，2023，42（1）：118-127.China Edible Fungi Association.Analysis of the results of the national edible fungi statistical survey in 2021［J］.Edible fungi of China，2023，42（1）：118-127 （in Chinese） .

张金霞，蔡为明，黄晨阳.中国食用菌栽培学［M］.北京：中国农业出版社，2020.ZHANG J X，CAI W M，HUANG C Y.Chinese edible mushroom cultivation［M］.Beijing：China Agriculture Press，2020 （in Chinese） .

金鑫，刘宗敏，黄羽佳，等.我国灵芝栽培现状及发展趋势［J］.食药用菌，2016，24（1）：33-37.JIN X，LIU Z M，HUANG Y J，et al.Current situation and development trend of Ganoderma lucidum cultivation in China［J］.Edible and medicinal mushrooms，2016，24（1）：33-37 （in Chinese） .

刘忱，张美玲，舒灿伟，等.真菌病毒的研究进展［J］.中国植保导刊，2016，36（9）：18-27.LIU C，ZHANG M L，SHU C W，et al.Research progress on mycovirus［J］.China plant protection，2016，36（9）：18-27 （in Chinese with English abstract） .

HOLLINGS M.Viruses associated with a die-back disease of cultivated mushroom［J］.Nature，1962，196（4858）：962-965.

HEINIGER U.Biological control of chestnut blight in Europe［J］.Annual review of phytopathology，1994，32：581-599.

GAO Z X，WU J Y，JIANG D H，et al. ORF Ι of mycovirus SsNSRV-1 is associated with debilitating symptoms of Sclerotinia sclerotiorum ［J/OL］.Viruses，2020，12（4）：456［2024-11-25］.https：//doi.org/10.3390/v12040456.

DIELEMAN-VAN ZAAYEN A.Intracellular appearance of mushroom virus in fruiting bodies and basidiospores of Agaricus bisporus ［J］.Virology，1972，47（1）：94-104.

SCHISLER L C， SINDEN J W， SIGEL E M.Etiology， symptomatology， and epidemiology of a virus disease of cultivated mushrooms［J］.Phytopathology， 1967， 57（4）： 519-526.

KIM J M，SONG H Y，CHOI H J，et al.Changes in the mycovirus （LeV） titer and viral effect on the vegetative growth of the edible mushroom Lentinula edodes ［J］.Virus research，2015，197：8-12.

RO H S，LEE N J，LEE C W，et al.Isolation of a novel mycovirus OMIV in Pleurotus ostreatus and its detection using a triple antibody sandwich-ELISA［J］.Journal of virological methods，2006，138（1/2）：24-29.

李雪飞，宋冰，李玉.食用菌病毒的研究进展［J］.微生物学报，2019，59（10）：1841-1854.LI X F，SONG B，LI Y.Research progress in mushroom virus［J］.Acta microbiologica sinica，2019，59（10）：1841-1854 （in Chinese with English abstract） .

KREUZE J F，PEREZ A，UNTIVEROS M，et al.Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs：a generic method for diagnosis，discovery and sequencing of viruses［J］.Virology，2009，388（1）：1-7.

DOLJA V V，KOONIN E V.Metagenomics reshapes the concepts of RNA virus evolution by revealing extensive horizontal virus transfer［J］.Virus research，2018，244：36-52.

WANG W Q，WANG X H，TU C Y，et al.Novel mycoviruses discovered from a metatranscriptomics survey of the phytopathogenic Alternaria fungus［J/OL］.Viruses，2022，14（11）：2552［2024-11-25］.https：//doi.org/10.3390/v14112552.

YE Y T，LIU Y Y，ZHANG Y F，et al.Metatranscriptome-based strategy reveals the existence of novel mycoviruses in the plant pathogenic fungus Fusarium oxysporum f.sp. cubense ［J/OL］.Frontiers in microbiology，2023，14：1193714［2024-11-25］.https：//doi.org/10.3389/fmicb.2023.1193714.

丛倩倩，兰玉菲，崔晓，等.利用siRNA高通量测序技术检测平菇病毒［J］.中国食用菌，2018，37（1）：61-64.CONG Q Q，LAN Y F，CUI X，et al.Detection of Pleurotus ostreatus virus by si RNA high-throughput sequencing［J］.Edible fungi of China，2018，37（1）：61-64 （in Chinese with English abstract） .

LIU H Z，ZHANG Y F，LIU Y Y，et al.Virome analysis of an ectomycorrhizal fungus Suillus luteus revealing potential evolutionary implications［J/OL］.Frontiers in cellular and infection microbiology，2023，13：1229859［2024-11-25］.https：//doi.org/10.3389/fcimb.2023.1229859.

YU H J，LIM D，LEE H S.Characterization of a novel single-stranded RNA mycovirus in Pleurotus ostreatus ［J］.Virology，2003，314（1）：9-15.

LI X F，LI S Y，YIN W Q，et al.Complete genome sequence of a novel mycovirus from Pleurotus citrinopileatus ［J/OL］.Archives of virology，2023，168（2）：66［2024-11-25］.https：//doi.org/10.1007/s00705-022-05668-4.

XIAO J B，WANG X，ZHENG Z R，et al.Molecular characterization of a novel deltaflexivirus infecting the edible fungus Pleurotus ostreatus ［J/OL］.Archives of virology，2023，168（6）：162［2024-11-25］.https：//doi.org/10.1007/s00705-023-05789-4.

MAGAE Y，SUNAGAWA M.Characterization of a mycovirus associated with the brown discoloration of edible mushroom， Flammulina velutipes ［J/OL］.Virology journal，2010，7：342［2024-11-25］.https：//doi.org/10.1186/1743-422x-7-342.

BRÄUTIGAM A，MULLICK T，SCHLIESKY S，et al.Critical assessment of assembly strategies for non-model species mRNA-Seq data and application of next-generation sequencing to the comparison of C（3） and C（4） species［J］.Journal of experimental botany，2011，62（9）：3093-3102.

GARG R，PATEL R K，TYAGI A K，et al. De novo assembly of chickpea transcriptome using short reads for gene discovery and marker identification［J］.DNA research，2011，18（1）：53-63.

ZHANG Y J，GAO J，LI Y.Diversity of mycoviruses in edible fungi［J］.Virus genes，2022，58（5）：377-391.

LI X F，SUI K P，XIE J T，et al.Molecular characterization of a novel fusarivirus infecting the edible fungus Auricularia heimuer ［J］.Archives of virology，2020，165（11）：2689-2693.

LI X F，LIU Q C，LI S Y，et al.A novel fungal negative-stranded RNA virus related to mymonaviruses in Auricularia heimuer ［J］.Archives of virology，2022，167（11）：2223-2227.

LIU C X，GUO M P，WANG J J，et al.Prevalence and diversity of mycoviruses occurring in Chinese Lentinula edodes germplasm resource［J］.Virology，2023，582：71-82.

GUO M P，SHEN G Y，WANG J J，et al.Mycoviral diversity and characteristics of a negative-stranded RNA virus LeNSRV1 in the edible mushroom Lentinula edodes ［J］.V irology，2021，555：89-101.

LIN Y H，FUJITA M，CHIBA S，et al.Two novel fungal negative-strand RNA viruses related to mymonaviruses and phenuiviruses in the shiitake mushroom （ Lentinula edodes ）［J］.Virology，2019，533：125-136.

GUO M P，BIAN Y B，WANG J J，et al.Biological and molecular characteristics of a novel partitivirus infecting the edible fungus Lentinula edodes ［J］.Plant disease，2017，101（5）：726-733.

MAGAE Y.Molecular characterization of a novel mycovirus in the cultivated mushroom， Lentinula edodes ［J/OL］.Virology journal，2012，9：60［2024-11-25］.https：//doi.org/10.1186/1743-422x-9-60.

WON H K，PARK S J，KIM D K，et al.Isolation and characterization of a mycovirus in Lentinula edodes ［J］.Journal of microbiology，2013，51（1）：118-122.

LIM W S，JEONG J H，JEONG R D，et al.Complete nucleotide sequence and genome organization of a dsRNA partitivirus infecting Pleurotus ostreatus ［J］.Virus research，2005，108（1/2）：111-119.

QIU L Y，LI Y P，LIU Y M，et al.Particle and naked RNA mycoviruses in industrially cultivated mushroom Pleurotus ostreatus in China［J］.Fungal biology，2010，114（5/6）：507-513.

SONG H Y，CHOI H J，JEONG H，et al.Viral effects of a dsRNA mycovirus （PoV-ASI2792） on the vegetative growth of the edible mushroom Pleurotus ostreatus ［J］.Mycobiology，2016，44（4）：283-290.

VAINIO E J，SUTELA S.Mixed infection by a partitivirus and a negative-sense RNA virus related to mymonaviruses in the polypore fungus Bondarzewia berkeleyi ［J/OL］.Virus research，2020，286：198079［2024-11-25］.https：//doi.org/10.1016/j.virusres.2020.198079.

HU H J，WANG J R，CHENG X H，et al.Preliminary studies on the effects of oyster mushroom spherical virus China strain on the mycelial growth and fruiting body yield of the edible mushroom Pleurotus ostreatus ［J/OL］.Biology，2022，11（4）：574［2024-11-25］.

ZHANG X Y，HU H J，ZHAO Y X，et al.Oyster mushroom spherical virus crosses the species barrier and is pathogenic to a new host Pleurotus pulmonarius ［J/OL］.International journal of molecular sciences，2023，24（13）：10584［2024-11-25］.https：//doi.org/10.3390/ijms241310584.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

生物质灰添加量对自蔓延合成基质块性能的影响

干湿交替灌溉对再生稻根系形态结构、根系活力与产量的影响

畜禽养殖废水生物处理及资源化技术研究进展

芥菜自交系重要农艺性状的遗传多样性分析

猪场沼液UF-NF-RO浓缩液水培生菜的研究