1.华中农业大学水产学院/双水双绿研究院, 武汉 430070
2.湖北洪山实验室, 武汉 430070
3.荆门(中国农谷)农业科学研究院, 荆门 448000
4.湖北省小龙虾产业技术研究院有限公司, 潜江 433100
黑春雨,E-mail:hcy123@webmail.hzau.edu.cn
顾泽茂,E-mail:guzemao@mail.hzau.edu.cn
收稿:2025-04-13,
纸质出版:2025-05-06
移动端阅览
黑春雨,陶杨,顾泽茂,等.不同育种策略对克氏原螯虾繁殖性能的影响[J].华中农业大学学报,2025,44(3):147‐155.
HEI Chunyu,TAO Yang,GU Zemao,et al.Effects of different breeding strategies on reproductive performance of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):147-155.
黑春雨,陶杨,顾泽茂,等.不同育种策略对克氏原螯虾繁殖性能的影响[J].华中农业大学学报,2025,44(3):147‐155. DOI: 10.13300/j.cnki.hnlkxb.2025.03.017.
HEI Chunyu,TAO Yang,GU Zemao,et al.Effects of different breeding strategies on reproductive performance of Procambarus clarkii[J].Journal of Huazhong Agricultural University,2025,44(03):147-155. DOI: 10.13300/j.cnki.hnlkxb.2025.03.017.
为了筛选克氏原螯虾(
Procambarus clarkii
)高效育种策略,解决优质种苗短缺问题,通过比较自繁自养、外源引种和群体选育3个育种策略形成克氏原螯虾种质群体的繁殖性能,并结合回归分析和结构方程
模型(structural equation modeling, SEM)解析影响繁殖性能的关键表型及其作用路径。结果显示,选育群体克氏原螯虾的绝对抱卵量[(621.35±173.43) 粒/尾]、相对抱卵量[(15.53±2.57) 粒/g]、卵粒总质量[(4.31±1.53) g]和抱卵率(94.7%)均显著高于外源引种群体[(374.35±85.75) 粒/尾,(13.15±2.42) 粒/g,(3.36±0.82) g,71.7%,
P
<
0.05]和自繁自养群体[(190.54±79.08) 粒/尾,(12.30±3.11) 粒/g,(1.44±0.67) g,52%,
P<
0.01);外源引种群体克氏原螯虾的绝对抱卵量显著高于自繁自养群体(
P
<
0.05),但二者之间的相对抱卵量差异并不显著。SEM分析显示,头胸甲宽对绝对抱卵量的标准化路径系数为0.542,高于体质量(0.451)、卵粒总质量(0.387)对绝对抱卵量的标准化路径系数,是影响绝对抱卵量的最关键表型。头胸甲宽通过影响体质量与卵粒总质量,间接提升了绝对抱卵量(CFI=0.918,GFI=0.917,RMSEA=0.056)。此外,选育群体克氏原螯虾的头胸甲宽[(2.52±0.19) cm]显著高于外源引种群体[(2.39±0.13) cm,
P
<
0.001]与自繁自养群体[(1.91±0.31) cm,
P
<
0.001],以上结果表明群体选育是提升克氏原螯虾繁殖性能的高效策略,头胸甲宽是群体选育提升克氏原螯虾繁殖性能的关键表型特征。
To screen effective breeding strategies for
Procambarus clarkii
and address the shortage of high-quality seedlings,this study compared the reproductive performance of germplasm populations formed through self-rearing,external introduction,and selective breeding.Additionally,and using regression analysis and structural equation modeling (SEM) were employed to elucidate the key phenotypic traits and their pathways influencing reproductive performance.The results showed that
P. clarkii
from the selectively bred population exhibited significantly higher absolute fecundity [(621.35±173.43) eggs/female],relative fecundity [(15.53±2.57) eggs/g],total egg mass [(4.31±1.53) g],and egg-carrying rate (94.7%) compared to individuals from the introduced population [(374.35±85.75) eggs/female,(13.15±2.42) eggs/g,(3.36±0.82) g,71.7%;
P
<
0.05] and the self-reared population [(190.54±79.08) eggs/female,(12.30±3.11) eggs/g,(1.44±0.67) g,52%;
P
<
0.01].The absolute fecundity of the introduced population was significantly higher than that of the self-reared population (
P
<
0.05),but there was no significant difference in their r
elative fecundity.SEM analysis revealed that the standardized path coefficient from carapace width to absolute fecundity (0.542) was higher than those from body weight (0.451) and total egg mass (0.387),indicating that carapace width is the most critical phenotypic trait influencing absolute fecundity.Carapace width indirectly enhanced absolute fecundity by influencing both body weight and total egg mass,with the model showing a good fit (CFI=0.918,GFI=0.917,RMSEA=0.056).Furthermore,the carapace width of the selectively bred population [(2.52±0.19) cm] was significantly greater than that of the introduced population [(2.39±0.13) cm,
P
<
0.001] and the self-reared population [(1.91±0.31) cm,
P
<
0.001].These results indicate that selective breeding is an effective strategy for enhancing the reproductive performance of
P. clarkii
,with carapace width being the key phenotypic trait responsible for this improvement.
LI Y H,GUO X W,CAO X J,et al.Population genetic structure and post-establishment dispersal patterns of the red swamp crayfish Procambarus clarkii in China[J/OL].PLoS One,2012,7(7):e40652[2025-04-13].https://doi.org/10.1371/journal.pone.0040652.
全国水产技术推广总站,中国水产学会. 中国小龙虾产业发展报告(2024)[J]. 中国水产, 2024(7): 14-20. National Fisheries Technology Extension Station,China Fisheries Society. China crayfish industry development report (2024) [J]. China fisheries, 2024(7): 14-20 (in Chinese) .
夏爱军,庞璐,严维辉,等.雌性克氏原螯虾性腺发育及相关系数的周年动态研究[J].淡水渔业,2008,38(4):12-15. XIA A J,PANG L,YAN W H,et al.Study on the mathematic model of gonad development and annual changes of relative coefficients of female Procambarus clarkii [J].Freshwater fisheries,2008,38(4):12-15 (in Chinese with English abstract) .
陈静,何吉祥,黄龙,等.克氏原螯虾形态性状、体质量与怀卵量的通径分析[J].南方农业,2024,18(1):232-235. CHEN J,HE J X,HUANG L,et al.Path analysis of morphological traits,body weight and egg carrying capacity of Procambarus clarkii [J].South China agriculture,2024,18(1):232-235 (in Chinese with English abstract) .
任妮,戴红君,张琤琤,等.我国克氏原螯虾产业调查分析与发展对策建议[J].江苏农业科学,2021,49(19):241-246. REN N,DAI H J,ZHANG C C,et al.Investigation and analysis of China’s crayfish industry and suggestions for development[J].Jiangsu agricultural sciences,2021,49(19):241-246 (in Chinese with English abstract) .
刘小宇,熊礼静,彭波,等.14个克氏原螯虾养殖群体遗传多样性分析[J].水产科学,2023,42(3):457-465. LIU X Y,XIONG L J,PENG B,et al.Genetic diversity analysis of 14 cultured populations of red swamp crayfish Procambarus clarkii in China[J].Fisheries science,2023,42(3):457-465 (in Chinese with English abstract) .
ZHANG J L,LIAO M C,WANG J H,et al.Development of microsatellite markers and comprehensive assessment on genetic variation of the red swamp crayfish ( Procambarus clarkii ) in Hubei Province,China[J].Aquaculture international,2024,32(4):5067-5083.
王长忠,李忠,梁宏伟,等. 长江下游地区4个克氏原螯虾群体的遗传多样性分析[J]. 生物多样性,2009,17(5): 518-523. WANG C Z,LI Z,LIANG H W,et al. Genetic diversity in four Procambarus clarkii populations in the lower reaches of the Yangtze River[J]. Biodiversity science,2009,17(5): 518-523 (in Chinese with English abstract) .
龚世园,吕建林,孙瑞杰,等.克氏原螯虾繁殖生物学研究[J].淡水渔业,2008,38(6):23-25. GONG S Y,LÜ J L,SUN R J,et al.Study on reproductive biology of Procambarus clarkii [J].Freshwater fisheries,2008,38(6):23-25 (in Chinese with English abstract) .
曹烈,杜时强,徐金根,等.4个不同水系克氏原螯虾繁殖与养殖性能对比[J].江西水产科技,2023(4):6-11.CAO L,DU S Q,XU J G,et al.Comparison of reproduction and culture performance of Procambarus clarkii in four different water systems[J].Jiangxi fishery science and technology,2023(4):6-11 (in Chinese) .
JIN S Y,JACQUIN L,HUANG F,et al.Optimizing reproductive performance and embryonic development of red swamp crayfish Procambarus clarkii by manipulating water temperature[J].Aquaculture,2019,510:32-42.
CARMONA-OSALDE C,RODRIGUEZ-SERNA M,OLVERA-NOVOA M A,et al.Gonadal development,spawning,growth and survival of the crayfish Procambarus llamasi at three different water temperatures[J].Aquaculture,2004,232(1/2/3/4):305-316.
刘永鑫,张殿福,陶忠虎,等.温度对克氏原螯虾胚胎和幼体发育的影响[J].华中农业大学学报,2021,40(5): 146-153.LIU Y X,ZHANG D F,TAO Z H,et al.Effects of temperature on embryonic and larval development of Procambarus clarkii [J].Journal of Huazhong Agricultural University,2021,40(5): 146-153 (in Chinese with English abstract) .
YU J X,XIONG M T,YE S W,et al.Effects of stocking density and artificial macrophyte shelter on survival,growth and molting of juvenile red swamp crayfish ( Procambarus clarkii ) under experimental conditions[J/OL].Aquaculture,2020,521:735001[2025-04-13].https://doi.org/10.1016/j.aquaculture.2020.735001.
宋光同,丁凤琴,陈静,等.亲虾规格、隐蔽物、光照度及密度对克氏原螯虾繁殖效果的影响[J].水产科学,2012,31(9): 549-553.SONG G T,DING F Q,CHEN J,et al.Effects of broodstock sizes,shelter,illumination and stocking density on breeding in red swamp crayfish Procambarus clarkii [J].Fisheries science,2012,31(9): 549-553 (in Chinese with English abstract) .
许荟,许志强,唐建清,等.干旱胁迫对克氏原螯虾亲虾生长、抗氧化能力及生殖相关指标的影响[J].江苏农业科学,2022,50(6):143-148.XU H,XU Z Q,TANG J Q,et al.Impacts of drought stress on growth,antioxidant capacity and reproduction-related indicators of parent crayfish Procambarus clarkia [J].Jiangsu agricultural sciences,2022,50(6):143-148 (in Chinese with English abstract) .
李庭古.盐度对克氏原螯虾孵化率的影响[J].水产科学,2009,28(12):789-791.LI T G.The effects of salinity on embryonic development of crayfish Procambarus clarkii [J].Fisheries science,2009,28(12):789-791 (in Chinese with English abstract) .
张金刚,吴怡迪,郭红会,等.中华绒螯蟹亲蟹群体形态性状对体质量的影响分析[J].海洋渔业,2024,46(6): 758-767.ZHANG J G,WU Y D,GUO H H,et al.Effects of morphological traits on body weight in parent population of Eriocheir sinensis [J].Marine fisheries,2024,46(6): 758-767 (in Chinese with English abstract) .
李东宇,潘志,顾志峰,等.红螯螯虾形态性状、体质量与其抱卵性状的通径分析[J].海南热带海洋学院学报,2022,29(2):1-7.LI D Y,PAN Z,GU Z F,et al.Path analysis of morphological traits,body weight and pleopodal egg traits of redclaw crayfish ( Cherax quadricarinatus )[J].Journal of Hainan Tropical Ocean University,2022,29(2):1-7 (in Chinese with English abstract) .
殷乐,姜晓东,成永旭,等.超大规格中华绒螯蟹繁殖性能和育苗效果评价[J].上海海洋大学学报,2024,33(3): 606-614.YIN L,JIANG X D,CHENG Y X,et al.Evaluation on reproductive performance and breeding effect of super-sized Chinese mitten crab ( Eriocheir sinensis ) [J].Journal of Shanghai Ocean University,2024,33(3): 606-614 (in Chinese with English abstract) .
TAN J,KONG J,CAO B X,et al.Genetic parameter estimation of reproductive traits of Litopenaeus vannamei [J].Journal of Ocean University of China,2017,16(1):161-167.
王亚威,姜晓东,吴旭干,等.中华绒螯蟹二龄早熟和晚熟群体选育第三代在扣蟹阶段的养殖性能评价[J].上海海洋大学学报,2016,25(5):668-674.WANG Y W,JIANG X D,WU X G,et al.The evaluation of culture performance of the second-year early-maturing and late-maturing strains of the third selevtive generation during the juvenile culture of Chinese mitten crab( Eriocheir sinensis )[J].Journal of Shanghai Ocean University,2016,25(5):668-674 (in Chinese with English abstract) .
孔杰,栾生,谭建 ,等.对虾选择育种研究进展[J].中国海洋大学学报(自然科学版),2020,50(9):81-97.KONG J,LUAN S,TAN J,et al.Progress of study on penaeid shrimp selective breeding[J].Periodical of Ocean University of China,2020,50(9):81-97 (in Chinese with English abstract) .
VU N T,NGUYEN N H.Quantitative genetic changes in reproductive performance of giant freshwater prawn after 10 years of selection for increased growth rate[J].Zuchthygiene,2019,54(2):199-206.
REN S J,MATHER P B,PRENTIS P,et al.Quantitative genetic assessment of female reproductive traits in a domesticated Pacific white shrimp ( Penaeus vannamei ) line in China[J/OL].Scientific reports,2020,10:7840[2025-04-13].https://doi.org/10.1038/s41598-020-64597-x.
胡倩,王齐帅,黄瑾,等.基于形态性状和SSR标记的克氏原螯虾养殖群体遗传多样性分析[J].华中农业大学学报,2024,43(3):258-266.HU Q,WANG Q S,HUANG J,et al.Genetic diversity analysis of farming Procambarus clarkii population based on morphology traits and SSR markers[J].Journal of Huazhong Agricultural University,2024,43(3):258-266 (in Chinese with English abstract) .
YUAN J B,YU Y,ZHANG X J,et al.Recent advances in crustacean genomics and their potential application in aquaculture[J].Aquaculture,2023,15(4):1501-1521.
庄振俊,唐美君,张冬冬,等.中华绒螯蟹“长荡湖1号” 连续3个世代的遗传多样性分析[J].水生生物学报,2023,47(9):1523-1533.ZHUANG Z J,TANG M J,ZHANG D D,et al.Genetic diversity of three consecutive generations of Eriocheir sinensis “Changdang Lake 1”[J].Acta hydrobiologica sinica,2023,47(9):1523-1533 (in Chinese with English abstract) .
彭敏,陈慧芳,李强勇,等.凡纳滨对虾连续3个世代选育群体的遗传多样性分析[J].南方农业学报,2020,51(6): 1442-1450.PENG M,CHEN H F,LI Q Y,et al.Genetic diversity of three consecutive generations of Litopenaeus vannamei [J].Journal of southern agriculture,2020,51(6): 1442-1450 (in Chinese with English abstract) .
彭涛,张冬冬,姜晓东,等.中华绒螯蟹“长荡湖1号”奇数年群体选育第二代的生长性能和养殖效果评估[J].中国水产科学,2023,30(10): 1177-1187.PENG T,ZHANG D D,JIANG X D,et al.Evaluation of growth and culture performance of the second generation of Chinese mitten crab ( Eriocheir sinensis ) [J].Journal of fishery sciences of China,2023,30(10): 1177-1187 (in Chinese with English abstract) .
陈琨,张林浩,姚子航,等.马氏珠母贝黑壳色选育系F5和对照群体生产性状的比较[J].中国水产科学,2023,30(11): 1293-1300. CHEN K,ZHANG L H,YAO Z H,et al.Comparison of production traits between the black shell color breeding line F5 and the control population of Pinctada fucata martensii [J].Journal of fishery sciences of China,2023,30(11): 1293-1300 (in Chinese with English abstract) .
王伦,王崇懿,刘建勇,等.凡纳滨对虾多代选育群体生长和耐综合胁迫性状的配合力及杂种优势分析[J].海洋学报,2022,44(4): 65-73.WANG L,WANG C Y,LIU J Y,et al.Analysis of combining ability and heterosis of growth and comprehensive stress tolerance traits of Litopenaeus vannamei multi-generation breeding population[J].Haiyang Xuebao,2022,44(4):65-73 (in Chinese with English abstract) .
LUO K,KONG J,LUAN S,et al.Effect of inbreeding on survival,WSSV tolerance and growth at the postlarval stage of experimental full-sibling inbred populations of the Chinese shrimp Fenneropenaeus chinensis [J].Aquaculture,2014,420:32-37.
TROPEA C,MARCIANO A,LÓPEZ GRECO L S.Brothers are better than nothing:first report of incestuous mating and inbreeding depression in a freshwater decapod crustacean[J/OL].Zoology,2022,151:125990[2025-04-13].https://doi.org/10.1016/j.zool.2021.125990.
王仁杰,姜令绪,李玉全.三疣梭子蟹形态指标、体质量与抱卵量的关系[J].江苏农业科学,2011,39(3): 300-302.WANG R J,JIANG L X,LI Y Q.Relationship between morphological indexes,body weight and egg-carrying capacity of swimming crab ( Portunus trituberculatus ) [J].Jiangsu agricultural sciences,2011,39(3): 300-302 (in Chinese) .
ATTARD J,HUDON C.Embryonic development and energetic investment in egg production in relation to size of female lobster ( Homarus americanus )[J].Canadian journal of fisheries and aquatic sciences,1987,44(6):1157-1164.
AAQILLAH-AMR M A,HIDIR A,NOORDIYANA M N,et al.Morphological,biochemical and histological analysis of mud crab ovary and hepatopancreas at different stages of development[J].Animal reproduction science,2018,195:274-283.
FENG W P,ZHAO Z P,WANG J T,et al.Nutrient composition of ovary,hepatopancreas and muscle tissues in relation to ovarian development stage of female swimming crab, Portunus trituberculatus [J/OL].Animals,2023,13(20):3220[2025-04-13].https://doi.org/10.3390/ani13203220.
0
浏览量
3
下载量
0
CSCD
关联资源
相关文章
相关作者
相关机构
京公网安备11010802024621