利用CO<sub>2</sub>合成挥发性脂肪酸的同型产乙酸反应的影响因素

申瑞延; 姚冬杰; 陈望学; 郑颖; 王媛媛

doi:10.13300/j.cnki.hnlkxb.2025.02.030

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利用CO₂合成挥发性脂肪酸的同型产乙酸反应的影响因素

食品与农业工程 | 更新时间：2026-11-05

- 利用CO₂合成挥发性脂肪酸的同型产乙酸反应的影响因素
- Factors affecting homoacetogenesis of volatile fatty acids with CO₂
- 华中农业大学学报 2025年44卷第2期页码：293-300
- 作者机构：
  
  华中农业大学工学院，武汉 430070
- 作者简介：
  
  申瑞延，E-mail：179469041@qq.com
  王媛媛，E-mail：wangyuanyuan@mail.hzau.edu.cn
- 基金信息：
  
  湖北省自然科学基金项目(2022CFB135)
- DOI：10.13300/j.cnki.hnlkxb.2025.02.030
  中图分类号： Q939.9;S216.3
- 收稿：2024-03-13，
  
  纸质出版：2025-03-06
- 稿件说明：
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申瑞延，姚冬杰，陈望学，等.利用CO₂合成挥发性脂肪酸的同型产乙酸反应的影响因素［J］.华中农业大学学报，2025，44（2）：293‐300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030.

SHEN Ruiyan,YAO Dongjie,CHEN Wangxue,et al.Factors affecting homoacetogenesis of volatile fatty acids with CO₂[J].Journal of Huazhong Agricultural University,2025,44(02):293-300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030.
申瑞延，姚冬杰，陈望学，等.利用CO₂合成挥发性脂肪酸的同型产乙酸反应的影响因素［J］.华中农业大学学报，2025，44（2）：293‐300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030. DOI：

SHEN Ruiyan,YAO Dongjie,CHEN Wangxue,et al.Factors affecting homoacetogenesis of volatile fatty acids with CO₂[J].Journal of Huazhong Agricultural University,2025,44(02):293-300. DOI: 10.13300/j.cnki.hnlkxb.2025.02.030. DOI：

摘要

为了能够以低成本的方式实现CO

的资源化利用，同时获得具有高价值的挥发性脂肪酸（volatile fatty acids，VFAs），利用厌氧污泥进行同型产乙酸菌的富集培养，并分别以葡萄糖、CO

和H

为底物，利用同型产乙酸菌的自养特性，探讨将CO

进行资源转化的能力以及顶空H

/CO

比例、碳源种类及pH值对同型产乙酸反应产物合成的影响。结果显示，高pH值有利于同型产乙酸菌利用H

/CO

混合气体进行自养反应，当pH=5、

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=76661440&type=

3.13266683

https://html.publish.founderss.cn/rc-pub/api/common/picture?pictureId=76661473&type=

14.22399998

=3∶1时的VFAs（包括乙酸、丙酸和丁酸）产量最高，达到3 369.89 mg/L，乙酸占比为76.1%。当以葡萄糖与H

/CO

混合气体作为共同碳源时，同型产乙酸菌会优先利用葡萄糖进行异养反应，随后开始利用H

/CO

混合气体。相关性分析表明，VFAs含量与pH值之间呈弱正相关，而与H

/CO

呈现出负相关趋势，表明CO

含量对同型产乙酸菌的自养途径影响更大。此外，建立的同型产乙酸反应的BP神经网络预测模型，可以准确预测以H

/CO

混合气体为碳源时同型产乙酸菌合成的VFAs产量（

=0.966）。

Abstract

Industrial syngas contains a large amount of CO

， and the utilization of CO

resources is of great significance to the current improvement of environment. The homoacetogenesis in the process of anaerobic fermentation has characteristics of autotrophy and heterotrophy. It can use CO

and H

to produce acetic acid， and degrade glucose to produce volatile fatty acids （VFAs）. The autotrophic characteristics of homoacetogenic bacteria were used to study its ability to convert CO

into resources and the effects of headspace H

/CO

ratio， the type of carbon sources and pH on the synthesis of homoacetogenic reaction products. The results showed that high pH was beneficial for homoacetogenic bacteria to utilize H

/CO

mixed gas for autotrophic reactions. When pH=5 and H

/CO

=3∶1， the yield of VFAs including acetic acid， propionic acid and butyric acid was the highest， reaching 3 369.89 mg/L， and the proportion of acetic acid was 76.1%. When glucose and H

/CO

mixed gas were used as a common carbon source， the homoacetogenic bacteria preferentially used glucose for heterotrophic reaction， and then the H

/CO

mixed gas began to be utilized. The results of correlation analysis showed that there was a weak positive correlation between the content of VFAs an

d the value of pH， but a negative correlation with H

/CO

， indicating that the content of CO

has a greater impact on the autotrophic pathway of homoacetogens. A prediction model with BP neural network for homoacetogenic reaction was established， which accurately predicted the yield of VFAs synthesized by homoacetogenic bacteria with H

/CO

mixed gas as the carbon source（

=0.966）.

关键词

Keywords

references

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利用CO2合成挥发性脂肪酸的同型产乙酸反应的影响因素

Factors affecting homoacetogenesis of volatile fatty acids with CO2

DOI：10.13300/j.cnki.hnlkxb.2025.02.030

摘要

Abstract

关键词

Keywords

references

利用CO₂合成挥发性脂肪酸的同型产乙酸反应的影响因素

Factors affecting homoacetogenesis of volatile fatty acids with CO₂