壳聚糖-苯二酚修饰灭活PEDV的口服免疫效果评价

杨云涵; 邓锴; 涂志文; 赵兴洪; 万红平

doi:10.13300/j.cnki.hnlkxb.2025.01.024

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壳聚糖-苯二酚修饰灭活PEDV的口服免疫效果评价

动物科学·动物医学 | 更新时间：2026-11-05

- 壳聚糖-苯二酚修饰灭活PEDV的口服免疫效果评价
- Evaluating oral immunization of inactivated PEDV vaccine modified with chitosan-catechol
- 华中农业大学学报 2025年44卷第1期页码：217-224
- 作者机构：
  
  四川农业大学动物医学院，成都 611130
- 作者简介：
  
  杨云涵，E-mail： yhyang123000@163.com；
  万红平，E-mail： hpwan@sicau.edu.cn
- 基金信息：
  
  国家自然科学基金项目(2122999007);中央引导地方科技发展专项(2212129410)
- DOI：10.13300/j.cnki.hnlkxb.2025.01.024
  中图分类号： S852.659.6
- 收稿：2024-07-28，
  
  纸质出版：2025-01-15
- 稿件说明：
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杨云涵，邓锴，涂志文，等.壳聚糖-苯二酚修饰灭活PEDV的口服免疫效果评价［J］.华中农业大学学报，2025，44（1）：217‐224.

YANG Yunhan,DENG Kai,TU Zhiwen,et al.Evaluating oral immunization of inactivated PEDV vaccine modified with chitosan-catechol[J].Journal of Huazhong Agricultural University,2025,44(01):217-224.
杨云涵，邓锴，涂志文，等.壳聚糖-苯二酚修饰灭活PEDV的口服免疫效果评价［J］.华中农业大学学报，2025，44（1）：217‐224. DOI： 10.13300/j.cnki.hnlkxb.2025.01.024.

YANG Yunhan,DENG Kai,TU Zhiwen,et al.Evaluating oral immunization of inactivated PEDV vaccine modified with chitosan-catechol[J].Journal of Huazhong Agricultural University,2025,44(01):217-224. DOI： 10.13300/j.cnki.hnlkxb.2025.01.024.

摘要

为评估壳聚糖-苯二酚（chitosan-catechol，Chic）修饰的灭活猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）疫苗的口服免疫效果，通过控制Chic的量制备出由不同质量浓度Chic（0.2、0.4 mg/mL）包裹的灭活PEDV（0.5 mg/mL），命名为C1+P和C2+P，建立小鼠模型进行免疫试验。采用间接酶联免疫吸附试验（ELISA）分别检测小鼠黏膜sIgA和血清IgG、IgG1、IgG2a抗体水平，并通过流式细胞术和CCK-8试验检测脾细胞的分型和增殖活化情况。结果显示，经2次口服免疫后，C2+P组诱导的免疫效果与昂贵的霍乱毒素B亚单位（CTB）佐剂组效果相当；与未经Chic修饰的PEDV组相比，C1+P、C2+P组诱导的鼻黏膜、肺泡、小肠黏膜特异性sIgA的抗体效价分别提高了6.09、6.37倍，5.35、5.65倍和5.64、5.89倍，血清特异性IgG、IgG1、IgG2a分别提高了8.83、9.65倍，13.54、15.07倍和4.27、5.07倍；CD4

T淋巴细胞、CD8

T淋巴细胞比例分别提升了1.15、1.17倍和1.11、1.11倍；脾细胞的增殖活化情况也显著增加（

0.05）。结果表明，口服免疫C1+P和C2+P成功诱导了小鼠体内黏膜免疫和系统性免疫应答效应，显示出Chic作为黏膜免疫佐剂的潜力，表明Chic修饰的灭活PEDV疫苗在有效诱导免疫应答方面具有可行性。

Abstract

Inactivated PEDV （0.5 mg/mL） encapsulated with different concentrations of chitosan-catechol（Chic） including 0.2 mg/mL and 0.4 mg/mL named as C1+P and C2+P was prepared by controlling the amount of Chic， and a mouse model was established for immunization testing to evaluate the oral immunization efficacy of inactivated porcine epidemic diarrhea virus （PEDV） vaccine modified with Chic. Indirect enz

yme-linked immunosorbent assay （ELISA） was used to detect the levels of sIgA in mouse mucosa and IgG， IgG1， IgG2a antibodies in serum. Flow cytometry and CCK-8 assay were used to detect the phenotyping and proliferation activation of spleen cells. The results showed that the immune response induced by the C2+P group was comparable to that of the expensive cholera toxin B subunit （CTB） adjuvant group after two oral immunizations. Compared with the PEDV group without Chic modification， the antibody titer of nasal，lung，and small intestine mucosa-specific sIgA in the C1+P group and C2+P group was increased by 6.09， 6.37 times， and 5.35， 5.65 times， and 5.64， 5.89 times， respectively. The serum specific IgG， IgG1， and IgG2a in the C1+P group and C2+P group increased by 8.83， 9.65 times， 13.54， 15.07 times， and 4.27， 5.07 times， respectively. The proportion of CD4

T lymphocytes and CD8

T lymphocytes in the C1+P group and C2+P group increased by 1.15， 1.17 times and 1.11，1.11 times， respectively. The proliferation and activation of spleen cells significantly increased as well （

0.05）. It is indicated that oral immunization with C1+P and C2+P successfully induced mucosal and systemic immune responses in mice， demonstrating the potential of Chic as a mucosal immune adjuvant and the feasibility of inactivated PEDV vaccines modified with Chic in effectively inducing immune responses.

关键词

Keywords

references

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