黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测

胡晓通; 代潇莹; 蔡远蓉; 程楚星; 张强; 金梅林

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黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测

更新时间：2026-10-15

- 黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测
- Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus
- 养殖与饲料 2026年页码：1-9
- 作者机构：
  
  华中农业大学动物科学技术学院、动物医学院，武汉 430070
- 作者简介：
  
  胡晓通，男，1997年生，博士。
  金梅林，女，1954年生，教授,博士生导师。
- 基金信息：
  
  国家重点研发计划(2025YFD1800700)
- DOI：
  中图分类号：
- 收稿：2026-09-02，
  
  网络首发：2026-10-15，
- 稿件说明：
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胡晓通,代潇莹,蔡远蓉等.黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测[J].养殖与饲料,

Hu Xiaotong,Dai Xiaoying,Cai Yuanrong,et al.Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus[J].Animals Breeding and Feed ,
胡晓通,代潇莹,蔡远蓉等.黄酮醇抗非洲猪瘟病毒活性及其作用靶点预测[J].养殖与饲料, DOI：10.13300/j.cnki.cn42-1648/s...001.

Hu Xiaotong,Dai Xiaoying,Cai Yuanrong,et al.Investigation of the antiviral activity and target prediction of flavonol against African swine fever virus[J].Animals Breeding and Feed , DOI：10.13300/j.cnki.cn42-1648/s...001.

摘要

目的

评价黄酮醇对非洲猪瘟病毒（ASFV）的体外抑制活性，并初步探讨其潜在作用靶点，为抗ASFV候选药物的筛选与开发提供参考依据。

方法

以猪肺泡巨噬细胞（PAMs）为细胞模型，采用细胞活力检测评价黄酮醇的细胞毒性；以Ⅱ型ASFV SY-1和Ⅰ/Ⅱ型重组ASFV 819为研究对象，检测不同浓度黄酮醇处理后病毒的复制水平，计算半数细胞毒性浓度（CC

）、半数抑制浓度（IC

）及选择指数（SI）；通过时间阶段试验分析黄酮醇的主要作用阶段，并采用AutoDock Vina进行黄酮醇与ASFV S273R蛋白的分子对接，分析预测结合能、结合构象及其与结合位点氨基酸残基之间的相互作用。

结果

黄酮醇处理PAMs 48 h后，10 μmol/L及以下浓度组细胞活率均高于90%。10 μmol/L黄酮醇对ASFV SY-1和819在PAMs上的复制均具有显著抑制作用。在PAMs中，黄酮醇的CC

为16.71 μmol/L，IC

为0.669 μmol/L，SI为24.98。时间阶段试验结果显示，黄酮醇主要在病毒与细胞共孵育阶段及感染后处理阶段发挥抑制作用。分子对接结果显示，黄酮醇与S273R蛋白的结合能为-25.9 kJ/mol，可与Asn187形成氢键，并与Pro194和His168形成π相互作用。

结论

黄酮醇在不明显影响PAMs细胞活力的浓度范围内能够抑制ASFV SY-1和819的复制，具有较好的体外抗ASFV活性。分子对接结果提示黄酮醇可能与pS273R蛋白发生相互作用，pS273R可能是其潜在作用靶点之一，但该推测仍需通过酶活实验、结合实验及关键位点突变实验进一步验证。

Abstract

Objectives

To evaluate the

in vitro

antiviral activity of flavonol against African swine fever virus

(ASFV) and explore its potential molecular targets

thereby providing a reference basis for the screening and development of candidate anti-ASFV drugs.

Methods

The cytotoxicity of flavonol was assessed in porcine alveolar macrophages (PAMs) by cell viability assay. Viral replication was measured in ASFV genotype Ⅱstrain SY-1 and recombinant genotype Ⅰ/Ⅱ strain 819 following treatment with serial flavonol concentrations. The 50% cytotoxic concentration (CC

)

50% inhibitory concentration (IC

)

and selectivity index (SI) were then determined. A time-of-addition assay identified the main stage of flavonol action

and molecular docking between flavonol and ASFV pS273R protein was performed with AutoDock Vina to analyze and predict the binding energy

binding conformations

and their interactions with the amino acid residues at the binding site.

Results

After 48 h of flavonol treatment

PAM cell viability remained above 90% at concentrations up to 10 μmol/L. Flavonol at 10 μmol/L significantly suppressed replication of both ASFV SY-1 and 819 in PAMs. The CC

and SI of flavonol were 16.71 μmol/L

0.669 μmol/L

and 24.98

respectively. A time-of-addition assay showed that flavonol acted mainly during virus-cell coincubation and post-infection stages. Molecular docking gave a binding energy of -25.9 kJ/mol between flavonol and S273R

with a hydrogen bond to Asn187 and π-interactions with Pro194 and His168.

Conclusions

Flavonol inhibits replication of ASFV SY-1 and 819 at concentrations that do not markedly affect PAM viability

showing favorable

in vitro

anti-ASFV activity. Molecular docking suggests that flavonol may interact with the pS273R protein

which could be one of its potential targets. This hypothesis nonetheless requires verification by enzyme activity assays

binding assays

and site-directed mutagenesis.

关键词

Keywords

references

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