1. 信阳农林学院体育学院,信阳
2. 郑州航空工业管理学院,郑州
3. 郑州大学第五附属医院康复科,郑州
纸质出版:2025
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吴金涛, 孙勇, 梁英姿, 等. 有氧运动对2型糖尿病大鼠内皮祖细胞功能及Akt/eNOS信号通路的影响[J]. 中华物理医学与康复杂志, 2025,47(7):595-600.
吴金涛, 孙勇, 梁英姿, et al. 有氧运动对2型糖尿病大鼠内皮祖细胞功能及Akt/eNOS信号通路的影响[J]. 2025, 47(7): 595-600.
目的探讨有氧运动对2型糖尿病大鼠内皮祖细胞(EPCs)功能及Akt/eNOS信号通路的影响。方法选取45只6周龄无特定病原体级(SPF)雄性SD大鼠
将其随机分为正常组、模型组及运动组。通过高脂饲料喂养、脲佐菌素注射将模型组及运动组大鼠制成2型糖尿病动物模型
运动组大鼠于制模成功后给予8周无负重游泳训练。待8周游泳训练结束后
通过腹主动脉取血检测各组大鼠外周血中EPCs数量以及血清一氧化氮(NO)、血管内皮生长因子(VEGF)含量
取股骨及胫骨分离培养骨髓EPCs
采用Matrigel管腔形成实验检测各组大鼠骨髓EPCs的成管能力
采用Western blot法检测各组大鼠EPCs中蛋白激酶B(Akt)及内皮型一氧化氮合酶(eNOS)蛋白表达量。结果与正常组比较
模型组外周血中EPCs计数及血清NO、VEGF含量均显著下降(P<0.05)
骨髓EPCs成管能力明显减弱(P<0.05)
磷酸化Akt(p-Akt)和p-eNOS蛋白表达均显著下调(P<0.05)。与模型组比较
运动组EPCs数量明显增加(P<0.05)
血清中NO及VEGF含量显著升高(P<0.05)
骨髓EPCs成管能力明显改善(P<0.05)
p-Akt及p-eNOS蛋白表达均显著上调(P<0.05)。结论有氧运动能改善糖尿病大鼠EPCs功能
其作用机制可能与调控Akt/eNOS信号通路有关。
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Kourek Christos,Briasoulis Alexandros,Zouganeli Virginia,Karatzanos Eleftherios,Nanas Serafim,Dimopoulos Stavros.Exercise Training Effects on Circulating Endothelial and Progenitor Cells in Heart Failure[J].Journal of Cardiovascular Development and Disease,2022.
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