Effects of Tunnel Construction on Microbial and Plant Derived Carbon Sources in Soil Aggregate Organic Carbon on the Eastern Margin of the Qinghai⁃Xizang Plateau

Lin Hongyu; Yu Du; Cao Huan; Wang Shuai; Li Lin; Pei Xiangjun; Tang Xiaolu

doi:10.3799/dqkx.2026.001

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Effects of Tunnel Construction on Microbial and Plant Derived Carbon Sources in Soil Aggregate Organic Carbon on the Eastern Margin of the Qinghai⁃Xizang Plateau

更新时间：2026-10-15

- Effects of Tunnel Construction on Microbial and Plant Derived Carbon Sources in Soil Aggregate Organic Carbon on the Eastern Margin of the Qinghai⁃Xizang Plateau
- Earth Science Vol. 51, Issue 9, Pages: 3332-3348(2026)
- 作者机构：
  
  1.天府永兴实验室，四川成都 610213
  2.成都理工大学生态环境学院，四川成都 610050
  3.成都理工大学地理与规划学院，四川成都 610050
- 作者简介：
- 基金信息：
- DOI：10.3799/dqkx.2026.001
  CLC： P642.5
- Received：30 October 2025，
  
  Published：30 September 2026
- 稿件说明：
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林洪羽，于杜，曹欢，王率，李凛，裴向军，唐晓鹿，2026.青藏高原东缘隧道建设对土壤团聚体有机碳微生物和植物碳源的影响.地球科学，51（9）：3332-3348.

Lin Hongyu，Yu Du，Cao Huan，Wang Shuai，Li Lin，Pei Xiangjun，Tang Xiaolu，2026.Effects of Tunnel Construction on Microbial and Plant Derived Carbon Sources in Soil Aggregate Organic Carbon on the Eastern Margin of the Qinghai⁃Xizang Plateau.Earth Science，51（9）：3332-3348.
林洪羽，于杜，曹欢，王率，李凛，裴向军，唐晓鹿，2026.青藏高原东缘隧道建设对土壤团聚体有机碳微生物和植物碳源的影响.地球科学，51（9）：3332-3348. DOI： 10.3799/dqkx.2026.001.

Lin Hongyu，Yu Du，Cao Huan，Wang Shuai，Li Lin，Pei Xiangjun，Tang Xiaolu，2026.Effects of Tunnel Construction on Microbial and Plant Derived Carbon Sources in Soil Aggregate Organic Carbon on the Eastern Margin of the Qinghai⁃Xizang Plateau.Earth Science，51（9）：3332-3348. DOI： 10.3799/dqkx.2026.001.

摘要

为探究青藏高原大型工程建设对土壤有机碳来源的影响，本研究以该区域某隧道为研究区，划定隧道建设影响区与非影响区，沿3 240 m、3 420 m、3 600 m三个不同海拔梯度，测定0~10 cm、10~30 cm土层深度土壤团聚体结构及其微生物（氨基糖）与植物来源（木质素），分析了土壤颗粒（>0.053 mm）及矿物结合态（<0.053 mm）团聚体对土壤有机碳来源的影响.结果表明，隧道建设对土壤团聚体氨基葡萄糖、氨基半乳糖、总氨基糖、真菌、微生物残体碳含量及木质素酚含量无显著影响，但显著降低了土壤团聚体胞壁酸、氨基甘露糖含量.0~10 cm土层中，隧道建设影响区与非建设影响区各采样点土壤颗粒态团聚体氨基糖含量随海拔升高无显著差异，而10~30 cm土层中，海拔3 420 m处的氨基糖含量显著低于海拔3 240 m和3 600 m处的氨基糖含量.海拔3 420 m土壤矿物结合态团聚体总氨基糖含量最低，显著低于其他海拔地区.同时，隧道影响区矿物结合态团聚体中，微生物残体碳和植物碳源对团聚体有机碳的平均贡献分别为26.36%和20.65%，显著低于非影响建设区，表明隧道建设降低了土壤团聚体稳定性，对矿物结合态团聚体的影响尤为明显.其中，矿物结合态团聚体在隧道建设、海拔梯度与土层深度的交互作用下，微生物残体碳含量及其对SOC的贡献率均显著降低，而土壤颗粒态团聚体有机碳的微生物、植物来源碳贡献均未受隧道建设显著影响.研究结果可为青藏高原大型工程扰动区土壤碳库保护与碳汇功能提升提供重要科学依据，对服务高原生态安全屏障建设和“双碳”战略实施具有重要意义.

Abstract

To investigate the impact of large-scale engineering on the sources of soil organic carbon (SOC) on the Qinghai-Xizang Plateau

this study was conducted along a tunnel corridor in the region. Tunnel-affected (TC) and unaffected (CK) sites at three elevations (3 240

3 420 and 3 600 m). Soil samples were collected from depths of 0-10 and 10-30 cm to determine aggregate structure and the contributions of microbial-derived carbon

indicated by amino sugars

and plant-derived carbon

indicated by lignin phenols

to SOC in the particulate (

0.053 mm) and mineral-associated (

0.053 mm) aggregate fractions. The results show that tunnel construction had no significant effect on glucosamine

galactosamine

total amino sugars

fungal necromass

carbon or total lignin phenols in soil aggregates (

0.05). However

it significantly reduced muramic acid

mannosamine

and bacterial residue carbon. In the particulate fraction

amino sugar contents did not differ significantly among elevations at 0-10 cm. There was no significant difference in amino sugars in soil particulate aggregates across different elevations at 0-10 cm soil depth. However

at 10-30 cm soil depth

amino sugar content at 3 420 m was significantly lower than that at 3 240 m and 3 600 m. For mineral-associated aggregates

amino sugar content at 3 420 m was lower than those at the other elevations. Meanwhile

in the mineral-bound aggregates of the tunnel-affected area

the average contributions of microbial residue carbon and lignin phenols to aggregate organic carbon were 26.36% and 20.65%

respectively

which were significantly lower than those in the control area (

0.05). The study indicates that tunnel construction reduces the stability of soil aggregates

especially that of mineral associated aggregates. Specifically

under the interactive effects of tunnel construction

altitude

and soil depth

the microbial residue carbon content of mineral-bound aggregates and their contribution rate to soil organic carbon decrease significantly

whereas microbial- and plant-derived carbon contributions to organic carbon in particulate aggregates were not significantly affected by tunnel construction. The findings highlight the importance of protecting and enhancing soil carbon pools in areas disturbed by large-scale engineering activities on the Qinghai-Xizang Plateau

with implications for strengthening the plateau’s ecological security barrier and advancing China’s “carbon neutral” goal.

关键词

Keywords

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