Characteristics of Soil Nutrient and Microbial Community Evolution in Ecologically Restored Areas Affected by Engineering Disturbance in High-Altitude Alpine Meadows

Li Qi; Zhang Pengfei; Shen Yifan; Li Yuhang; Jin Bo; Wei Renjie; Luo Jing; Zhang Weibiao; Zhang Xiaochao; Lei Ningfei; Pei Xiangjun

doi:10.3799/dqkx.2025.302

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Characteristics of Soil Nutrient and Microbial Community Evolution in Ecologically Restored Areas Affected by Engineering Disturbance in High-Altitude Alpine Meadows

更新时间：2026-10-15

- Characteristics of Soil Nutrient and Microbial Community Evolution in Ecologically Restored Areas Affected by Engineering Disturbance in High-Altitude Alpine Meadows
- Earth Science Vol. 51, Issue 9, Pages: 3309-3319(2026)
- 作者机构：
  
  1.成都理工大学地质灾害防治与地质环境保护全国重点实验室，四川成都 610059
  2.成都理工大学生态环境学院，四川成都 610059
  3.天府永兴实验室，四川成都 610213
  4.成都理工大学环境与土木工程学院，四川成都 610059
  5.中铁二十四局集团有限公司，上海 200433
- 作者简介：
- 基金信息：
- DOI：10.3799/dqkx.2025.302
  CLC： P69
- Received：30 August 2025，
  
  Published：30 September 2026
- 稿件说明：
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李琪，张鹏飞，沈逸凡，李宇航，金博，魏人杰，罗璟，张卫彪，张晓超，雷泞菲，裴向军，2026.高寒草甸工程扰损区生态重构土壤养分与微生物群落演变特征.地球科学，51（9）：3309-3319.

Li Qi，Zhang Pengfei，Shen Yifan，Li Yuhang，Jin Bo，Wei Renjie，Luo Jing，Zhang Weibiao，Zhang Xiaochao，Lei Ningfei，Pei Xiangjun，2026.Characteristics of Soil Nutrient and Microbial Community Evolution in Ecologically Restored Areas Affected by Engineering Disturbance in High⁃Altitude Alpine Meadows.Earth Science，51（9）：3309-3319.
李琪，张鹏飞，沈逸凡，李宇航，金博，魏人杰，罗璟，张卫彪，张晓超，雷泞菲，裴向军，2026.高寒草甸工程扰损区生态重构土壤养分与微生物群落演变特征.地球科学，51（9）：3309-3319. DOI： 10.3799/dqkx.2025.302.

Li Qi，Zhang Pengfei，Shen Yifan，Li Yuhang，Jin Bo，Wei Renjie，Luo Jing，Zhang Weibiao，Zhang Xiaochao，Lei Ningfei，Pei Xiangjun，2026.Characteristics of Soil Nutrient and Microbial Community Evolution in Ecologically Restored Areas Affected by Engineering Disturbance in High⁃Altitude Alpine Meadows.Earth Science，51（9）：3309-3319. DOI： 10.3799/dqkx.2025.302.

摘要

工程扰动加速高寒草甸退化，开展工程扰损区草甸修复有助于维持区域生态系统稳定.以汪布曲草甸道路边坡生态重构土壤为研究对象，探究生态修复3年内土壤养分与微生物群落特征的变化规律.结果表明，修复后土壤有机碳、总氮、铵态氮和速效钾含量显著提升，分别增加了344.6%、95.8%、139.9%和174.1%.微生物群落多样性持续提高，酸杆菌、绿弯菌、拟杆菌和芽单胞菌丰度则随时间呈上升趋势.同时，土壤脲酶、蛋白酶及蔗糖酶活性显著增强.PLS-PM分析揭示，修复时间是驱动土壤有机碳和养分恢复的核心因子，其通过影响微生物群落结构‒土壤酶活性实现土壤与微生物的协同恢复.本研究可为高寒草甸扰损区土壤恢复效果评估提供数据支撑.

Abstract

Engineering disturbances accelerate the degradation of alpine meadows

but restoring degraded areas through ecological remediation helps maintain regional ecosystem stability. This study investigates changes in soil nutrients and microbial community characteristics over three years of ecological restoration

using soil from the ecologically restored roadside slopes in the Wangbuqu Meadow as the research subject. Results indicate that organic carbon

total nitrogen

ammonium nitrogen

and available potassium content in post-restoration soil significantly increased by 344.6%

95.8%

139.9%

and 174.1%

respectively. Microbial community diversity progressively enhanced

with rising temporal trends in the abundance of Acidobacteria

Chloroflexi

Bacteroidetes

and Gemmatimonadetes. Concurrently

soil urease

protease

and sucrase activities markedly intensified. Partial Least Squares-Path Modeling (PLS-PM) analysis reveals that remediation duration served as the primary driver for soil organic carbon and nutrient recovery

facilitating synergistic restoration of soil and microbial communities by influencing microbial community structure and soil enzyme activity. This study provides empirical support for soil recovery outcomes evaluation in disturbed alpine meadow ecosystems.

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