Identification of the <italic style="font-style: italic">BnACA</italic> family and preliminary functional analysis of <italic style="font-style: italic">BnaC08.ACA1</italic> in <italic style="font-style: italic">Brassica napus </italic>L.

LIU Xiang; ZHANG Jie; ZHANG Hong-chang; HUANG Zhen; LI Ke-qi

doi:10.19802/j.issn.1007-9084.2026349

您当前的位置：

首页 >

文章列表页 >

Identification of the BnACA family and preliminary functional analysis of BnaC08.ACA1 in Brassica napus L.

更新时间：2026-11-03

- Identification of the BnACA family and preliminary functional analysis of BnaC08.ACA1 in Brassica napus L.
- Chinese Journal of Oil Crop Sciences Vol. 48, Issue 5, Pages: 77-86(2026)
- 作者机构：
  
  西北农林科技大学农学院，陕西杨陵，712100
- 作者简介：
- 基金信息：
- DOI：10.19802/j.issn.1007-9084.2026349
  CLC： S565.4
- Received：10 October 2026，
  
  Published：25 October 2026
- 稿件说明：
移动端阅览
刘翔,张洁,张宏昌等.甘蓝型油菜BnACA家族的鉴定与BnaC08.ACA1的初步功能分析[J].中国油料作物学报,2026,48(05):77-86.

LIU Xiang,ZHANG Jie,ZHANG Hong-chang,et al.Identification of the BnACA family and preliminary functional analysis of BnaC08.ACA1 in Brassica napus L.[J].Chinese Journal of Oil Crop Sciences,2026,48(05):77-86.
刘翔,张洁,张宏昌等.甘蓝型油菜BnACA家族的鉴定与BnaC08.ACA1的初步功能分析[J].中国油料作物学报,2026,48(05):77-86. DOI： 10.19802/j.issn.1007-9084.2026349.

LIU Xiang,ZHANG Jie,ZHANG Hong-chang,et al.Identification of the BnACA family and preliminary functional analysis of BnaC08.ACA1 in Brassica napus L.[J].Chinese Journal of Oil Crop Sciences,2026,48(05):77-86. DOI： 10.19802/j.issn.1007-9084.2026349.

摘要

碳酸酐酶（carbonic anhydrase，CA）是催化二氧化碳和水生成碳酸氢根与氢离子的关键酶，参与生物体内无机碳转运、pH稳态调节等多种生命过程。本研究对甘蓝型油菜基因组中α-碳酸酐酶基因家族（alpha carbonic anhydrase，ACA）成员进行了鉴定，共鉴定到25个

BnACA

基因，按照进化关系分为4个亚族，分布于16条染色体/染色体片段上。启动子序列分析结果表明，

BnACA

启动子区有大量激素响应、非生物胁迫相关的顺式作用元件。不同非生物胁迫下的表达量分析结果表明，

BnaC08.ACA1

表达受ABA、干旱、盐、高温胁迫的影响。为进一步研究

BnaC08.ACA1

的功能，本研究克隆了该基因并在拟南芥中异源过表达。抗旱性鉴定结果表明，过表达

BnaC08.ACA1

降低了拟南芥苗期的抗旱性，表明

BnaC08.ACA1

可能是干旱响应的负调控因子。

Abstract

Carbonic anhydrase (CA) is a key enzyme that catalyzes the reversible reaction of carbon dioxide and water to produce bicarbonate and hydrogen ions

participating in various biological processes such as inorganic car

bon transport and pH homeostasis in organisms. In this study

members of the alpha carbonic anhydrase (ACA) gene family were identified from the

Brassica napus

genome. A total of 25

BnACA

genes were identified

which were classified into four subfamilies based on phylogenetic relationships and distributed across 16 chromosomes/chromosome fragments. Promoter sequence analysis revealed that the

BnACAs

promoters contain abundant

cis

-acting elements associated with hormone responses and abiotic stresses. Expression profiling under different abiotic stresses showed that the expression of

BnaC08.ACA1

is affected by ABA

drought

salt

and heat stresses. To further investigate the function of

BnaC08.ACA1

this gene was cloned and heterologously overexpressed in

Arabidopsis thaliana

. Drought resistance assays demonstrated that overexpression of

BnaC08.ACA1

reduced drought resistance of

Arabidopsis

seedlings

indicating that

BnaC08.ACA1

might act as a negative regulator in drought response.

关键词

Keywords

references

Hu D D ， Jing J J ， Snowdon R J ， et al . Exploring the gene pool of Brassica napus by genomics-based approaches ［J］. Plant Biotechnol J ， 2021 ， 19 （ 9 ）： 1693 - 1712 . DOI： 10.1111/pbi.13636 http://dx.doi.org/10.1111/pbi.13636 .

Wang L M ， Jin X ， Li Q B ， et al . Comparative proteomics reveals that phosphorylation of β carbonic anhydrase 1 might be important for adaptation to drought stress in Brassica napus ［J］. Sci Rep ， 2016 ， 6 ： 39024 . DOI： 10.1038/srep39024 http://dx.doi.org/10.1038/srep39024 .

冯海棠，王汉中 . 新形势下的我国食用植物油供给安全对策［J］. 中国油料作物学报， 2024 ， 46 （ 2 ）： 221 - 227 . DOI： 10.19802/j.issn.1007-9084.2024021 http://dx.doi.org/10.19802/j.issn.1007-9084.2024021 .

Amiri-Oghan H ， Fotokian M H ， Javidfar F ， et al . Genetic analysis of grain yield， days to flowering and maturity in oilseed rape （ Brassica napus L.） using diallel crosses ［J］. Int J Plant Prod ， 2009 ， 3 （ 2 ）： 19 - 26 . DOI： doi：10.1111/j.1757-1707.2009.01012.x http://dx.doi.org/doi：10.1111/j.1757-1707.2009.01012.x .

Omidi H ， Tahmasebi Z ， Naghdi Badi H A ， et al . Fatty acid composition of canola （ Brassica napus L.）， as affected by agronomical， genotypic and environmental parameters ［J］. C R Biol ， 2010 ， 333 （ 3 ）： 248 - 254 . DOI： 10.1016/j.crvi.2009.10.001 http://dx.doi.org/10.1016/j.crvi.2009.10.001 .

Wang J ， Mao L ， Li Y Y ， et al . Natural variation in BnaA9.NF-YA7 contributes to drought tolerance in Brassica napus L .［J］. Nat Commun ， 2024 ， 15 （ 1 ）： 2082 . DOI： 10.1038/s41467-024-46271-2 http://dx.doi.org/10.1038/s41467-024-46271-2 .

Gao S F ， Xu J S ， Song W ， et al . Overexpression of BnMYBL2-1 improves plant drought tolerance via the ABA-dependent pathway ［J］. Plant Physiol Biochem ， 2024 ， 207 ： 108293 . DOI： 10.1016/j.plaphy.2023.108293 http://dx.doi.org/10.1016/j.plaphy.2023.108293 .

Gupta A ， Rico-Medina A ， Caño-Delgado A I . The physiology of plant responses to drought ［J］. Science ， 2020 ， 368 （ 6488 ）： 266 - 269 . DOI： 10.1126/science.aaz7614 http://dx.doi.org/10.1126/science.aaz7614 .

杨海云，艾雪莹， Batool Maria ，等 . 油菜响应水分胁迫的生理机制及栽培调控措施研究进展［J］. 华中农业大学学报， 2021 ， 40 （ 2 ）： 6 - 16 . DOI： 10.13300/j.cnki.hnlkxb.2021.02.002 http://dx.doi.org/10.13300/j.cnki.hnlkxb.2021.02.002 .

朱波，徐绮雯，马淑敏，等 . 干旱胁迫下施钾水平对油菜生长特性、籽粒品质和钾素利用的影响［J］. 植物营养与肥料学报， 2021 ， 27 （ 6 ）： 1016 - 1026 . DOI： 10.11674/zwyf.20561 http://dx.doi.org/10.11674/zwyf.20561 .

Alber B E ， Ferry J G . A carbonic anhydrase from the archaeon Methanosarcina thermophila ［J］. Proc Natl Acad Sci U S A ， 1994 ， 91 （ 15 ）： 6909 - 6913 . DOI： 10.1073/pnas.91.15.6909 http://dx.doi.org/10.1073/pnas.91.15.6909 .

Wang L ， Liang J ， Zhou Y ， et al . Molecular characterization of carbonic anhydrase genes in Lotus japonicus and their potential roles in symbiotic nitrogen fixation ［J］. Int J Mol Sci ， 2021 ， 22 （ 15 ）： 7766 . DOI： 10.3390/ijms22157766 http://dx.doi.org/10.3390/ijms22157766 .

DiMario R J ， Machingura M C ， Waldrop G L ， et al . The many types of carbonic anhydrases in photosynthetic organisms ［J］. Plant Sci ， 2018 ， 268 ： 11 - 17 . DOI： 10.1016/j.plantsci.2017.12.002 http://dx.doi.org/10.1016/j.plantsci.2017.12.002 .

Supuran C T . Carbonic anhydrases： novel therapeutic applications for inhibitors and activators ［J］. Nat Rev Drug Discov ， 2008 ， 7 （ 2 ）： 168 - 181 . DOI： 10.1038/nrd2467 http://dx.doi.org/10.1038/nrd2467 .

Nadeeva E M ， Ignatova L K ， Rudenko N N ， et al . Features of photosynthesis in Arabidopsis thaliana plants with knocked out gene of alpha carbonic anhydrase 2 ［J］. Plants ， 2023 ， 12 （ 9 ）： 1763 . DOI： 10.3390/plants12091763 http://dx.doi.org/10.3390/plants12091763 .

Villarejo A ， Burén S ， Larsson S ， et al . Evidence for a protein transported through the secretory pathway en route to the higher plant chloroplast ［J］. Nat Cell Biol ， 2005 ， 7 （ 12 ）： 1224 - 1231 . DOI： 10.1038/ncb1330 http://dx.doi.org/10.1038/ncb1330 .

Fenner N ， Freeman C . Drought-induced carbon loss in peatlands ［J］. Nat Geosci ， 2011 ， 4 （ 12 ）： 895 - 900 . DOI： 10.1038/ngeo1323 http://dx.doi.org/10.1038/ngeo1323 .

Raines C ， Cavanagh A P ， Afamefule C ， et al . Instant insights： optimising photosynthesis in crops ［M］. Burleigh Dodds Science Publishing ， 2023 .

Caruso G ， Cavaliere C ， Foglia P ， et al . Analysis of drought responsive proteins in wheat （ Triticum durum ） by 2D-PAGE and MALDI-TOF mass spectrometry ［J］. Plant Sci ， 2009 ， 177 （ 6 ）： 570 - 576 . DOI： 10.1016/j.plantsci.2009.08.007 http://dx.doi.org/10.1016/j.plantsci.2009.08.007 .

Kaul T ， Reddy P S ， Mahanty S ， et al . Biochemical and molecular characterization of stress-induced β-carbonic anhydrase from a C 4 plant， Pennisetum glaucum ［J］. J Plant Physiol ， 2011 ， 168 （ 6 ）： 601 - 610 . DOI： 10.1016/j.jplph.2010.08.007 http://dx.doi.org/10.1016/j.jplph.2010.08.007 .

Sun R R ， Wang Y Y ， Zhu R H ， et al . Genome-wide identification of CA genes in cotton and the functional analysis of GhαCA4-D， GhβCA6-D and GhγCA2-D in response to drought and salt stresses ［J］. Int J Biol Macromol ， 2025 ， 304 ： 140872 . DOI： 10.1016/j.ijbiomac.2025.140872 http://dx.doi.org/10.1016/j.ijbiomac.2025.140872 .

Chen C J ， Wu Y ， Li J W ， et al . TBtools-II： a “one for all， all for one” bioinformatics platform for biological big-data mining ［J］. Mol Plant ， 2023 ， 16 （ 11 ）： 1733 - 1742 . DOI： 10.1016/j.molp.2023.09.010 http://dx.doi.org/10.1016/j.molp.2023.09.010 .

Capasso C ， Supuran C T . Introduction to carbonic anhydrase ［M］// Carbonic Anhydrase Inhibitors . Cham ： Springer ， 2026 ： 1 - 15 . DOI： 10.1007/978-3-032-23172-7_1 http://dx.doi.org/10.1007/978-3-032-23172-7_1 .

DiMario R J ， Clayton H ， Mukherjee A ， et al . Plant carbonic anhydrases： structures， locations， evolution， and physiological roles ［J］. Mol Plant ， 2017 ， 10 （ 1 ）： 30 - 46 . DOI： 10.1016/j.molp.2016.09.001 http://dx.doi.org/10.1016/j.molp.2016.09.001 .

Fedorchuk T P ， Kireeva I A ， Opanasenko V K ， et al . Alpha carbonic anhydrase 5 mediates stimulation of ATP synthesis by bicarbonate in isolated Arabidopsis thylakoids ［J］. Front Plant Sci ， 2021 ， 12 ： 662082 . DOI： 10.3389/fpls.2021.662082 http://dx.doi.org/10.3389/fpls.2021.662082 .

Moroney J V ， Ma Y B ， Frey W D ， et al . The carbonic anhydrase isoforms of Chlamydomonas reinhardtii ： intracellular location， expression， and physiological roles ［J］. Photosynth Res ， 2011 ， 109 （ 1 ）： 133 - 149 . DOI： 10.1007/s11120-011-9635-3 http://dx.doi.org/10.1007/s11120-011-9635-3 .

Fabre N ， Reiter I M ， Becuwe-Linka N ， et al . Characterization and expression analysis of genes encoding alpha and beta carbonic anhydrases in Arabidopsis ［J］. Plant Cell Environ ， 2007 ， 30 （ 5 ）： 617 - 629 . DOI： 10.1111/j.1365-3040.2007.01651.x http://dx.doi.org/10.1111/j.1365-3040.2007.01651.x .

Langella E ， Di Fiore A ， Alterio V ， et al . α-CAs from photosynthetic organisms ［J］. Int J Mol Sci ， 2022 ， 23 （ 19 ）： 12045 . DOI： 10.3390/ijms231912045 http://dx.doi.org/10.3390/ijms231912045 .

Gao Y ， Zhao C . Genome-wide identification， structural features and single-cell expression atlas of the carbonic anhydrase gene family in maize （ Zea mays L.）［J］. bioRxiv ， 2025 ， doi： https：//doi.org/10.1101/2025.09.21.677582 http://dx.doi.org/https：//doi.org/10.1101/2025.09.21.677582 .

Rudenko N N ， Ignatova L K ， Nadeeva-Zhurikova E M ， et al . Advances in understanding the physiological role and locations of carbonic anhydrases in C 3 plant cells ［J］. Protoplasma ， 2021 ， 258 （ 2 ）： 249 - 262 . DOI： 10.1007/s00709-020-01566-1 http://dx.doi.org/10.1007/s00709-020-01566-1 .

孙润润，李佳慧，曹媛媛，等 . 碳酸酐酶 GhαCA3-D 调控陆地棉干旱胁迫的初步功能分析［J］. 西南农业学报， 2026 ， 39 （ 5 ）： 1054 - 1063 . DOI： 10.16213/j.cnki.scjas.2026.5.012 http://dx.doi.org/10.16213/j.cnki.scjas.2026.5.012 .

Rudenko N N ， Ruppert M Y ， Ignatova L K ， et al . Features of pho tosynthesis in Arabidopsis thaliana plants with knocked out genes encoding chloroplast carbonic anhydrases αCA1 and βCA1 ［J］. Biochemistry Moscow ， 2025 ， 90 （ 7 ）： 894 - 910 . DOI： 10.1134/s0006297925600954 http://dx.doi.org/10.1134/s0006297925600954 .

Jiao Q ， Xin L ， Bai H ， et al . Heterologous expression of α-AtCAH1 improves resistance to drought stress and increases tuber yield in potato （ Solanum tuberosum L.）［J］. Plant Biol J ， 2026 ， 28 （ 1 ）： 91 - 101 . DOI： 10.1111/plb.70141 http://dx.doi.org/10.1111/plb.70141 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Genome-wide identification and expression analysis of the SRS gene family in Brassica napus

Research and utilization of saline-alkali-tolerant Brassica napus—Review and prospect on two decades of research on saline-alkali-tolerant rapeseed at Huazhong Agricultural University

Research progress of U-box E3 ubiquitin ligase on regulation of drought resistance and other abiotic stresses in Brassica napus L.

Effects of cold, drought, and combined stresses on the morphology and physiological characteristics of peanut seedlings

Genome-wide identification and expression in response to salt-alkali stress of the SUT gene family in Glycine max

Related Author

ZHANG Yong-shun

WANG Hong-yan

WANG Long

XIAO Lu

FU Ting-dong

WAN He-ping

WEN Jing

SHEN Jin-xiong

Related Institution

Academy of Agricultural and Forestry Sciences, Qinghai University/Qinghai Spring Rape Engineering Research Center/Spring Rape Scientific Observation Experimental Station of Ministry of Agriculture and Rural Affairs/Qinghai Research Branch of the National Rapeseed Genetic Improvement Center/Key Laboratory of Qinghai Province for Spring Rapeseed Genetic Improvement

Laboratory of Plant Epigenetics and Evolution, School of Life Science, Liaoning University

College of Life Sciences, Jianghan University

College of Plant Science & Technology of Huazhong Agricultural University

Graduate School of Chinese Academy of Agricultural Sciences

AI问答

⁰

Identification of the BnACA family and preliminary functional analysis of BnaC08.ACA1 in Brassica napus L.

DOI：10.19802/j.issn.1007-9084.2026349

摘要

Abstract

关键词

Keywords

references