Genome-wide association study of root traits in <italic style="font-style: italic">Brassica napus </italic>L<italic style="font-style: italic">.</italic> seedlings under salt stress

ZHANG Wei; LUO Sheng; YANG Ting-hai; CHEN Feng; ZHOU Xiao-ying; FU San-xiong; ZHANG Jie-fu; WANG Xiao-dong; SHEN Yue

doi:10.19802/j.issn.1007-9084.2026294

您当前的位置：

首页 >

文章列表页 >

Genome-wide association study of root traits in Brassica napus L. seedlings under salt stress

更新时间：2026-11-03

- Genome-wide association study of root traits in Brassica napus L. seedlings under salt stress
- Chinese Journal of Oil Crop Sciences Vol. 48, Issue 5, Pages: 53-63(2026)
- 作者机构：
  
  江苏省农业科学院，江苏南京，210014
- 作者简介：
- 基金信息：
- DOI：10.19802/j.issn.1007-9084.2026294
  CLC： S565.4
- Received：31 August 2026，
  
  Published：25 October 2026
- 稿件说明：
移动端阅览
张维,罗升,杨廷海等.盐胁迫下甘蓝型油菜苗期根系性状的全基因组关联分析[J].中国油料作物学报,2026,48(05):53-63.

ZHANG Wei,LUO Sheng,YANG Ting-hai,et al.Genome-wide association study of root traits in Brassica napus L. seedlings under salt stress[J].Chinese Journal of Oil Crop Sciences,2026,48(05):53-63.
张维,罗升,杨廷海等.盐胁迫下甘蓝型油菜苗期根系性状的全基因组关联分析[J].中国油料作物学报,2026,48(05):53-63. DOI： 10.19802/j.issn.1007-9084.2026294.

ZHANG Wei,LUO Sheng,YANG Ting-hai,et al.Genome-wide association study of root traits in Brassica napus L. seedlings under salt stress[J].Chinese Journal of Oil Crop Sciences,2026,48(05):53-63. DOI： 10.19802/j.issn.1007-9084.2026294.

摘要

土壤盐渍化是制约油菜生产的主要非生物胁迫因子，根系作为感知盐胁迫的首要器官，其构型与耐盐性密切相关。为解析盐胁迫下甘蓝型油菜根系性状的遗传机制，以202份甘蓝型油菜种质资源为材料，测定盐胁迫下相对根长、相对根表面积、相对根体积和相对根尖数4个性状，结合全基因组重测序数据，采用混合线性模型进行全基因组关联分析。结果表明，4个性状表型变异丰富且呈极显著正相关；共获得120个高置信度关联SNP，整合为70个候选区间，结合转录组与基因注释初步筛选到6个根系耐盐候选基因；经主成分分析和隶属函数综合评价，筛选出6份强耐盐种质。本研究为解析甘蓝型油菜根系耐盐分子机制提供了新位点和候选基因。

Abstract

Soil salinization is a major abiotic stress restricting rapeseed production. As the primary organ sensing salt stress

root architecture is closely associated with salt tolerance. To dissect the genetic mechanism underlying salt tolerance in roots of

Brassica napus

a natural population consisting of 202

B. napus

accessions was used as material. 4 root salt-tolerance traits

including relative root length

relative root surface area

relative root volume and relative root tip number

were measured under salt stress. Combined with resequencing data

a mixed linear model was adopted to perform genome-wide association study. The results revealed abundant phenotypic variation in the 4 root traits

with extremely significant positive correlations observed among them. A total of 120 significantly associated single-nucleotide polymorphisms (SNPs) were detected

which were integrated into 70 candidate association intervals. 6 candidate genes responsible for root salt tolerance were ultimately selected by integrating transcriptomic data and gene functional annotation. Comprehensive evaluation of root salt tolerance was performed using principal component analysis and membership function

and 6 germplasm resources with strong salt tol

erance were screened out. This study provides novel loci and candidate genes for dissecting the molecular genetic mechanism of root salt tolerance in

B. napus

关键词

Keywords

references

Food and Agriculture Organization of the United Nations . Global status of salt-affected soils - Main report ［R］. 2024 . DOI： 10.4060/cd3044en.

于功霞，李迎，刘宏元，等 . 我国盐碱地改良与利用技术研究进展［J］. 黑龙江农业科学， 2025 （ 7 ）： 96 - 103 . DOI： 10.11942/j.issn1002-2767.2025.07.0096 http://dx.doi.org/10.11942/j.issn1002-2767.2025.07.0096 .

刘成，冯中朝，肖唐华，等 . 我国油菜产业发展现状、潜力及对策［J］. 中国油料作物学报， 2019 ， 41 （ 4 ）： 485 - 489 . DOI： 10.7505/j.issn.1007-9084.2019.04.001 http://dx.doi.org/10.7505/j.issn.1007-9084.2019.04.001 .

Shokri-Gharelo R ， Noparvar P M . Molecular response of canola to salt stress： insights on tolerance mechanisms ［J］. PeerJ ， 2018 ， 6 ： e4822 . DOI： 10.7717/peerj.4822 http://dx.doi.org/10.7717/peerj.4822 .

Lang L N ， Xu A X ， Ding J ， et al . Quantitative trait locus mapping of salt tolerance and identification of salt-tolerant genes in Brassica napus L ［J］. Front Plant Sci ， 2017 ， 8 ： 1000 . DOI： 10.3389/fpls.2017.01000 http://dx.doi.org/10.3389/fpls.2017.01000 .

侯林涛，王腾岳，荐红举，等 . 甘蓝型油菜盐胁迫下幼苗鲜重和干重QTL定位及候选基因分析［J］. 作物学报， 2017 ， 43 （ 2 ）： 179 - 189 . DOI： 10.3724/SP.J.1006.2017.00179 http://dx.doi.org/10.3724/SP.J.1006.2017.00179 .

蒙姜宇，梁光伟，贺亚军，等 . 甘蓝型油菜耐盐和耐旱相关性状的QTL分析［J］. 作物学报， 2021 ， 47 （ 3 ）： 462 - 471 . DOI： 10.3724/SP.J.1006.2021.04034 http://dx.doi.org/10.3724/SP.J.1006.2021.04034 .

Wassan G M ， Khanzada H ， Zhou Q H ， et al . Identification of genetic variation for salt tolerance in Brassica napus using genome-wide association mapping ［J］. Mol Genet Genom ， 2021 ， 296 （ 2 ）： 391 - 408 . DOI： 10.1007/s00438-020-01749-8 http://dx.doi.org/10.1007/s00438-020-01749-8 .

Zhang G F ， Zhou J Z ， Peng Y ， et al . Genome-wide association studies of salt tolerance at seed germination and seedling stages in Brassica napus ［J］. Front Plant Sci ， 2021 ， 12 ： 772708 . DOI： 10.3389/fpls.2021.772708 http://dx.doi.org/10.3389/fpls.2021.772708 .

黄紫红，范世航，华玮，等 . 甘蓝型油菜耐盐性全基因组关联分析与种质筛选［J］. 中国油料作物学报， 2025 ， 47 （ 6 ）： 1387 - 1398 . DOI： 10.19802/j.issn.1007-9084.2024302 http://dx.doi.org/10.19802/j.issn.1007-9084.2024302 .

Yang T H ， Zhou X Y ， Cao Y ， et al . Identification of genetic loci for salt tolerance in Brassica napus at the germination stage using a genome-wide association study ［J］. Ind Crops Prod ， 2024 ， 221 ： 119319 . DOI： 10.1016/j.indcrop.2024.119319 http://dx.doi.org/10.1016/j.indcrop.2024.119319 .

Wang W C ， Pang J Y ， Zhang F H ， et al . Salt-responsive transcriptome analysis of canola roots reveals candidate genes involved in the key metabolic pathway in response to salt stress ［J］. Sci Rep ， 2022 ， 12 （ 1 ）： 1666 . DOI： 10.1038/s41598-022-05700-2 http://dx.doi.org/10.1038/s41598-022-05700-2 .

El-Badri A M ， Batool M ， A A Mohamed I ， et al . Antioxidative and metabolic contribution to salinity stress responses in two rapeseed cultivars during the early seedling stage ［J］. Antioxidants ， 2021 ， 10 （ 8 ）： 1227 . DOI： 10.3390/antiox10081227 http://dx.doi.org/10.3390/antiox10081227 .

Yu L Q ， Iqbal S ， Zhang Y T ， et al . Proteome-wide ident ification of S-sulphenylated cysteines in Brassica napus ［J］. Plant Cell Environ ， 2021 ， 44 （ 11 ）： 3571 - 3582 . DOI： 10.1111/pce.14160 http://dx.doi.org/10.1111/pce.14160 .

张琪，李玲玉，唐乐，等 . 油菜耐盐机理与分子育种应用研究进展［J］. 作物研究， 2026 ， 40 （ 1 ）： 93 - 102 . DOI： 10.3969/j.issn.1001-5280.2026.01.012 http://dx.doi.org/10.3969/j.issn.1001-5280.2026.01.012 .

Lynch J . Root architecture and plant productivity ［J］. Plant Physiol ， 1995 ， 109 （ 1 ）： 7 - 13 . DOI： 10.1104/pp.109.1.7 http://dx.doi.org/10.1104/pp.109.1.7 .

Loudet O ， Gaudon V ， Trubuil A ， et al . Quantitative trait lo ci controlling root growth and architecture in Arabidopsis thaliana confirmed by heterogeneous inbred family ［J］. Theor Appl Genet ， 2005 ， 110 （ 4 ）： 742 - 753 . DOI： 10.1007/s00122-004-1900-9 http://dx.doi.org/10.1007/s00122-004-1900-9 .

Zhang Y ， Thomas C L ， Xiang J X ， et al . QTL meta-analysis of root traits in Brassica napus under contrasting phosphorus supply in two growth systems ［J］. Sci Rep ， 2016 ， 6 ： 33113 . DOI： 10.1038/srep33113 http://dx.doi.org/10.1038/srep33113 .

Arifuzzaman M ， Rahman M . Genome wide association mapping and candidate gene mining for root architectural traits in rapeseed/canola （ Brassica napus L.） at late growth stage ［J］. Euphytica ， 2020 ， 216 （ 10 ）： 164 . DOI： 10.1007/s10681-020-02700-z http://dx.doi.org/10.1007/s10681-020-02700-z .

Shi R ， Cao Y ， Yang T H ， et al . Genome-wide association study reveals the genetic basis of crude fiber components in Brassica napus l. shoots at stem elongation stage ［J］. J Agric Food Chem ， 2024 ， 72 （ 29 ）： 16530 - 16540 . DOI： 10.1021/acs.jafc.4c03032 http://dx.doi.org/10.1021/acs.jafc.4c03032 .

Yang T H ， Zhang W ， Shen Y ， et al . Genetic dissection of salt tolerance architecture in Brassica napus at the seedling stage by genome-wide association study ［J］. Oil Crop Sci ， 2026 ， 11 （ 2 ）： 138 - 149 . DOI： 10.1016/j.ocsci.2026.03.001 http://dx.doi.org/10.1016/j.ocsci.2026.03.001 .

武晓玲，梁海媛，杨峰，等 . 大豆苗期耐荫性综合评价及其鉴定指标的筛选［J］. 中国农业科学， 2015 ， 48 （ 13 ）： 2497 - 2507 . DOI： 10.3864/j.issn.0578-1752.2015.13.002 http://dx.doi.org/10.3864/j.issn.0578-1752.2015.13.002 .

Dai R ， Zhan N ， Geng R D ， et al . Progress on salt tolerance in Brassica napus ［J］. Plants ， 2024 ， 13 （ 14 ）： 1990 . DOI： 10.3390/plants13141990 http://dx.doi.org/10.3390/plants13141990 .

Swetha R ， Sridhanya V M ， Varanavasiappan S ， et al . Root apoplastic barrier mechanism： an adaptive strategy to protect against salt stress ［J］. Mol Biol Rep ， 2024 ， 52 （ 1 ）： 56 . DOI： 10.1007/s11033-024-10171-x http://dx.doi.org/10.1007/s11033-024-10171-x .

Zhou H P ， Shi H F ， Yang Y Q ， et al . Insights into plant salt stress signaling and tolerance ［J］. J Genet Genom ， 2024 ， 51 （ 1 ）： 16 - 34 . DOI： 10.1016/j.jgg.2023.08.007 http://dx.doi.org/10.1016/j.jgg.2023.08.007 .

Guzha A ， McGee R ， Scholz P ， et al . Cell wall-localized BETA-XYLOSIDASE4 contributes to immunity of Arabidopsis against Botrytis cinerea ［J］. Plant Physiol ， 2022 ， 189 （ 3 ）： 1794 - 1813 . DOI： 10.1093/plphys/kiac165 http://dx.doi.org/10.1093/plphys/kiac165 .

Keereetaweep J ， Blancaflor E B ， Hornung E ， et al . Lipoxygenase-derived 9-hydro（ Pero ） x ides of linoleoylethanolamide interact with ABA signaling to arrest root development during Arabidopsis seedling establishment ［J］. Plant J ， 2015 ， 82 （ 2 ）： 315 - 327 . DOI： 10.1111/tpj.12821 http://dx.doi.org/10.1111/tpj.12821 .

Rodriguez-Villalon A ， Gujas B ， van Wijk R ， et al . Primary root protophloem differentiation requires balanced phosphatidylinositol-4， 5-biphosphate levels and systemically affects root branching ［J］. Development ， 2015 ， 142 （ 8 ）： 1437 - 1446 . DOI： 10.1242/dev.118364 http://dx.doi.org/10.1242/dev.118364 .

Cui W E ， Lee J Y . Arabidopsis callose synthases CalS1/8 regulate plasmodesmal permeability during stress ［J］. Nat Plants ， 2016 ， 2 ： 16034 . DOI： 10.1038/nplants.2016.34 http://dx.doi.org/10.1038/nplants.2016.34 .

Lin Q F ， Wang S ， Dao Y H ， et al . Arabidopsis thaliana trehalose-6-phosphate phosphatase gene TPPI enhances drought tolerance by regulating stomatal apertures ［J］. J Exp Bot ， 2020 ， 71 （ 14 ）： 4285 - 4297 . DOI： 10.1093/jxb/eraa173 http://dx.doi.org/10.1093/jxb/eraa173 .

Mueller S ， Hilbert B ， Dueckershoff K ， et al . General detoxification and stress responses are mediated by oxidized lipids through TGA transcription factors in Arabidopsis ［J］. Plant Cell ， 2008 ， 20 （ 3 ）： 768 - 785 . DOI： 10.1105/tpc.107.054809 http://dx.doi.org/10.1105/tpc.107.054809 .

Zhu J K . Plant salt tolerance ［J］. Trends Plant Sci ， 2001 ， 6 （ 2 ）： 66 - 71 . DOI： 10.1016/S1360-1385（00）01838-0 http://dx.doi.org/10.1016/S1360-1385（00）01838-0 .

Platten J D ， Cotsaftis O ， Berthomieu P ， et al . Nomenclature for HKT transporters， key determinants of plant salinity tolerance ［J］. Trends Plant Sci ， 2006 ， 11 （ 8 ）： 372 - 374 . DOI： 10.1016/j.tplants.2006.06.001 http://dx.doi.org/10.1016/j.tplants.2006.06.001 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

QTL mapping and germplasm screening of alkaline tolerance in rapeseed (Brassica napus L.) during germination stage

Genome-wide association analysis of soybean resistance to whitefly

Genome wide association analysis of thousand-seed weight in Brassica napus L.

Physiological and genetic basis of saline-alkali tolerance in Brassica napus: from evolutionary origins to nutritional physiology and genetic dissection

Loss-of-function mutations of genes encoding BnaBIN2 kinases enhance cold tolerance of Brassica napus seedling

Related Author

GAO Xu

LIU Wei

WANG Yu-bin

LI Wei

WANG Cai-jie

ZHANG Li-feng

ZHANG Yan-wei

XU Ran

Related Institution

Crop Research Institute, Shandong Academy of Agricultural Sciences

College of Agronomy, Jiangxi Agricultural University

Jiangxi Institute of Red Soil / Jiangxi Key Laboratory of Red Soil Arable Land Conservation / National Engineering and Technology Research Center for Red Soil Improvement

Jiujiang University

National Key Laboratory of Crop Genetic Improvement, National Engineering Research Center of Rapeseed, Huazhong Agricultural University

AI问答

⁰

Genome-wide association study of root traits in Brassica napus L. seedlings under salt stress

DOI：10.19802/j.issn.1007-9084.2026294

摘要

Abstract

关键词

Keywords

references