Histological phenotypic characteristics during parasitic structure development of different<italic style="font-style: italic"> Orobanche cumana</italic> races

LIU Rong; WANG Ming-dong; YAN Ning-ning; LI Tie; LIU Rui; YANG Jian-feng; ZHANG Jian; CAO Qing-qin; XIAO Ting-ting; ZHAO Jun

doi:10.19802/j.issn.1007-9084.2026029

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Histological phenotypic characteristics during parasitic structure development of different Orobanche cumana races

更新时间：2026-11-03

- Histological phenotypic characteristics during parasitic structure development of different Orobanche cumana races
- Chinese Journal of Oil Crop Sciences Vol. 48, Issue 5, Pages: 220-229(2026)
- 作者机构：
  
  1.内蒙古农业大学园艺与植物保护学院，内蒙古呼和浩特，010019
  2.北京农学院植物科学技术学院园艺系，北京，102206
  3.塔里木大学农学院，新疆阿拉尔，843300
- 作者简介：
- 基金信息：
- DOI：10.19802/j.issn.1007-9084.2026029
  CLC： S565.5;S451;S453
- Received：29 January 2026，
  
  Published：25 October 2026
- 稿件说明：
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刘荣,王明东,闫宁宁等.向日葵列当不同生理小种寄生结构形成过程的组织表型特征[J].中国油料作物学报,2026,48(05):220-229.

LIU Rong,WANG Ming-dong,YAN Ning-ning,et al.Histological phenotypic characteristics during parasitic structure development of different Orobanche cumana races[J].Chinese Journal of Oil Crop Sciences,2026,48(05):220-229.
刘荣,王明东,闫宁宁等.向日葵列当不同生理小种寄生结构形成过程的组织表型特征[J].中国油料作物学报,2026,48(05):220-229. DOI： 10.19802/j.issn.1007-9084.2026029.

LIU Rong,WANG Ming-dong,YAN Ning-ning,et al.Histological phenotypic characteristics during parasitic structure development of different Orobanche cumana races[J].Chinese Journal of Oil Crop Sciences,2026,48(05):220-229. DOI： 10.19802/j.issn.1007-9084.2026029.

摘要

为探究列当（

Orobanche cumana

）不同生理小种在寄生向日葵过程中木质部桥的形成差异是否决定其寄生效率和发育时序，本研究以向日葵品

种LD5009为寄主，选取向日葵列当的D、E、G小种及寄生番茄的G小种为材料，结合振荡切片与双荧光染色技术，系统比较了不同生理小种在吸器形成、木质部桥发育及瘤结形成等关键阶段的组织学特征，揭示了木质部桥结构的复杂度与列当寄生能力之间的相关性。列当不同生理小种接种后的寄生瘤结的统计结果表明：相同寄生条件下，列当不同生理小种对向日葵根系的寄生能力的排序表现为G小种的寄生能力最强，寄生在番茄植株的G小种与D小种寄生能力次之，而E小种的寄生能力表现为最弱。按照列当寄生的不同阶段，在组织学水平比较了列当不同生理小种侵染后建立寄生结构过程差异，结果表明，接触期列当不同生理小种萌发形成的芽管与向日葵根系开始附着，附着后寄主根系结构无显著差异；吸器形成期，列当G小种和寄生在番茄植株上的向日葵G小种形成了高度发达的木质部桥结构，木质部桥组织在寄生组织中的占比远高于D、E小种。列当G小种和寄生在番茄植株上的G小种已经进入瘤结期，而D小种和E小种还处于寄生吸器的形成期。供试列当不同生理小种和寄生在番茄植株上的G小种从种子萌发形成芽管接触向日葵根系开始，23 d后均进入列当的出茎期。但是，寄生在番茄植株上的G小种的出茎最早，其次为列当G小种和D小种，而列当E小种的出茎最晚。本研究的结果还表明，不同列当生理小种在侵染向日葵根系时寄生组织的形态变化基本一致，但列当不同生理小种寄生时在向日葵根系形成的维管束桥的连接形态有一定的差别，主要表现在D、E小种的木质部桥与向日葵根系木质部细胞的连接结构的复杂度低，空间延伸范围窄，组织连通程度有限；而G小种及寄生在番茄植株上的G小种的木质部桥连接结构完整、空间延伸宽泛、组织连通性更充分，其连接形态与列当茎的形成和生长速度直接相关。

Abstract

To investigate whether the differences in xylem bridge formation among different physiological races of

Orobanche cumana

during sunflower parasitism determine their parasitic efficiency and developmental timing

this study used sunflower variety LD5009 as the host and selected races D

G (originating from sunflower)

and the tomato-adapted race G as materials. By combining vibratome sectioning with dual fluorescence staining technology

we systematically compared the histological characteristics of different races at key parasitic stages

including haustorium formation

xylem bridge development

and tubercle formation. This revealed a correlation between structural complexity of xylem bridge and parasitic ability. Statistical results on parasitic tubercles following inoculation with different races showed that under the same parasitic conditions

the order of parasitic ability on sunflower roots was as follows: race G (originating from sunflower) exhibited the strongest parasitic ability

followed by the tomato adapted race G and race D

while race E was the weakest. Histological comparisons across different parasitic stages revealed differences in the process of establishing parasitic structures after

infection by different races. The results indicated that at the attachment phase

germ tubes formed by different physiological races began attaching to sunflower roots

with no significant differences observed in host root structure post attachment. During the haustorium formation phase

race G (from sunflower) and the tomato adapted race G developed highly elaborate xylem bridge structures

whose proportion within the parasitic tissue was far greater than that of the corresponding structures formed by races D and E. When race G (from sunflower) and the tomato adapted race G had entered the tubercle phase

races D and E were still in the parasitic haustorium formation phase. All tested

O. cumana

races and the tomato adapted race G entered the stem emergence phase 23 d after seed germination and initial contact of the germ tube with sunflower roots. However

the tomato adapted race G exhibited the earliest stem emergence

followed by race G (from sunflower) and race D

while race E emerged latest. The above findings further revealed that the morphological changes of parasitic tissues during sunflower root infection were largely consistent among different

O. cumana

races

but the connection morphology of the vascular bridges formed in roots differed among races. Specifically

the xylem bridges of races D and E exhibited lower structural complexity in their connections to sunflower root xylem cells

narrower spatial extension

and limited tissue connectivity. In contrast

the xylem bridges of race G (from sunflower) and the tomato adapted race G were complete

exhibited broad spatial extension

and demonstrated more sufficient tissue connectivity. Their connection morphology was directly correlated with the subsequent formation and growth rate of

Orobanche

stem.

关键词

Keywords

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Histological phenotypic characteristics during parasitic structure development of different Orobanche cumana races

DOI：10.19802/j.issn.1007-9084.2026029

摘要

Abstract

关键词

Keywords

references