Localization of peanut genes associated with early flowering based on BSA-seq technology

WANG Fang; LYU Jiao-yan; XING Xiao-ning; WANG Hai-li; REN Rui; LU Lian-yong; WANG Ji-guo; WANG Yan; ZHANG Shao-cong; SHEN Xi-hua

doi:10.19802/j.issn.1007-9084.2026026

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Localization of peanut genes associated with early flowering based on BSA-seq technology

更新时间：2026-11-03

- Localization of peanut genes associated with early flowering based on BSA-seq technology
- Chinese Journal of Oil Crop Sciences Vol. 48, Issue 5, Pages: 114-122(2026)
- 作者机构：
  
  1.安阳市农业科学院，河南安阳，455000
  2.河南农业大学农学院，河南郑州，450046
- 作者简介：
- 基金信息：
- DOI：10.19802/j.issn.1007-9084.2026026
  CLC：
- Received：28 January 2026，
  
  Published：25 October 2026
- 稿件说明：
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王芳,吕娇艳,邢晓宁等.基于BSA-seq技术定位花生早花相关基因位点[J].中国油料作物学报,2026,48(05):114-122.

WANG Fang,LYU Jiao-yan,XING Xiao-ning,et al.Localization of peanut genes associated with early flowering based on BSA-seq technology[J].Chinese Journal of Oil Crop Sciences,2026,48(05):114-122.
王芳,吕娇艳,邢晓宁等.基于BSA-seq技术定位花生早花相关基因位点[J].中国油料作物学报,2026,48(05):114-122. DOI： 10.19802/j.issn.1007-9084.2026026.

WANG Fang,LYU Jiao-yan,XING Xiao-ning,et al.Localization of peanut genes associated with early flowering based on BSA-seq technology[J].Chinese Journal of Oil Crop Sciences,2026,48(05):114-122. DOI： 10.19802/j.issn.1007-9084.2026026.

摘要

为解析花生开花期调控的分子机制并筛选相关的候选基因，助力花生早花的分子育种，本研究以花生早花与晚花表型F₂分离群体为材料，构建极端性状混池，采用BSA-seq技术开展早花性状关联位点定位。结果显示，在4条染色体的相应区间内共鉴定到20个与早花性状显著关联的区间。这些染色体和区间包括A06染色体的23.50~98.50 Mb、A07染色体的62.00~63.00 Mb、B05染色体的14.00~14.10 Mb，和B08染色体的13.05~13.25 Mb区间。通过GO功能注释、KEGG通路富集分析及数据库多重比对，初步筛选出10个潜在开花相关候选基因；结合转录组表达数据进一步验证，最终确定2个候选基因

Arahy.M2THPA

与

Arahy.UD4HAV

。

Abstract

To elucidate molecular mechanism underlying flowering time regulation in peanut (

Arachis hypogaea

L.)

and to screen candidate genes associated with early flowering for breeding on early-flowering peanut

this study took an F₂ segregating population with contrasting early and late flowering phenotypes in peanut as material

and constructed extreme trait bulks. Authors adopted Bulked Segregant Analysis sequencing (BSA-seq) to map the loci associated with early-flowering trait. Results showed that a total of 20 intervals significa

ntly associated with early flowering were identified in 4 genomic regions

including 23.50-98.50 Mb on chromosome A06

62.00-63.00 Mb on chromosome A07

14.00-14.10 Mb on chromosome B05

and 13.05-13.25 Mb on chromosome B08. 10 potential flowering-related candidate genes were preliminarily screened

via

Gene Ontology (GO) functional annotation

Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis and multiple database alignment. Further validation combined with transcriptomic expression data ultimately identified 2 candidate genes:

Arahy.M2THPA

and

Arahy.UD4HAV

关键词

Keywords

references

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