Regulatory effects of <italic style="font-style: italic">Huaiqihuang</italic> on the differentiation of CD4<sup>+</sup> T cells and HaCaT keratinocytes <italic style="font-style: italic">in vitro</italic>

XIAO Guifeng; DENG Yaxuan; ZOU Yanfei; ZHENG Kaiping; WU Jianbo; LIU Qin

doi:10.14188/j.1671-8852.2025.0857

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Regulatory effects of Huaiqihuang on the differentiation of CD4⁺ T cells and HaCaT keratinocytes in vitro

更新时间：2026-11-06

- Regulatory effects of Huaiqihuang on the differentiation of CD4⁺ T cells and HaCaT keratinocytes in vitro
- Medical Journal of Wuhan University Vol. 46, Issue 12, Pages: 1537-1543(2025)
- 作者机构：
  
  武汉大学中南医院皮肤科湖北武汉 430071
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2025.0857
  CLC： R758.63
- Received：28 July 2025，
  
  Published：25 December 2025
- 稿件说明：
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肖桂凤,邓雅轩,邹燕菲等.槐杞黄对CD4⁺ T细胞及HaCaT角质形成细胞体外分化调控作用[J].武汉大学学报（医学版）,2025,46(12):1537-1543. DOI: 10.14188/j.1671-8852.2025.0857.

XIAO Guifeng,DENG Yaxuan,ZOU Yanfei,et al.Regulatory effects of Huaiqihuang on the differentiation of CD4⁺ T cells and HaCaT keratinocytes in vitro[J].Medical Journal of Wuhan University,2025,46(12):1537-1543. DOI: 10.14188/j.1671-8852.2025.0857.
肖桂凤,邓雅轩,邹燕菲等.槐杞黄对CD4⁺ T细胞及HaCaT角质形成细胞体外分化调控作用[J].武汉大学学报（医学版）,2025,46(12):1537-1543. DOI: 10.14188/j.1671-8852.2025.0857. DOI：

XIAO Guifeng,DENG Yaxuan,ZOU Yanfei,et al.Regulatory effects of Huaiqihuang on the differentiation of CD4⁺ T cells and HaCaT keratinocytes in vitro[J].Medical Journal of Wuhan University,2025,46(12):1537-1543. DOI: 10.14188/j.1671-8852.2025.0857. DOI：

摘要

目的

探索槐杞黄对体外培养的CD4

T细胞及HaCaT角质形成细胞分化增殖的影响。

方法

磁珠分选小鼠脾脏CD4

T细胞并分组培养，分别为：①对照组（标准条件培养CD4

T细胞），②阳性活化组（CD4

T细胞+抗CD3、抗CD28及IL‐6、IL‐23、TGF‐β1、IL‐1β、IL‐4刺激细胞分化），③实验药物组（阳性活化+槐杞黄）。采用流式细胞术检测各组CD4

T细胞表面活化标记（CD28及CD69）表达差异及Th17细胞分化标记ROR‐γ表达差异，采用ELISA法检测IL‐17A表达差异。体外分组培养HaCaT细胞，分别为：①对照组（标准条件培养HaCaT细胞），②阳性活化组（HaCaT细胞+IL‐22刺激HaCaT细胞增殖），③实验药物组（HaCaT细胞+IL‐22+槐杞黄）。采用Western Blot法检测各组JAK2、STAT3、Cyclin D1、C‐MYC表达差异。

结果

槐杞黄干预后，CD4

T细胞活化特征表面标记（CD28及CD69）明显升高（

0.01），Th17细胞分化标记（ROR‐γ及IL‐17A）的表达明显下降（

0.000 1）。HaCaT细胞中JAK2、p‐JAK2、STAT3、p‐STAT3、Cyclin D1、C‐MYC的表达均明显下降（

0.000 1）。

结论

槐杞黄可促进CD4

T细胞活化，但可抑制其向Th17细胞转化，降低IL‐17A的表达，并调控JAK2/STAT3通路抑制角质形成细胞的增殖，本研究为其治疗银屑病提供新的理论依据。

Abstract

Objective

To investigate the effects of

Huaiqihuang

a traditional Chinese herbal complex

on the differentiation and proliferation of CD4

T cells and HaCaT keratinocytes

in vitro

Methods

CD4

T cells were isolated from mouse spleens using magnetic bead sorting and divided into three groups for culture: ① Control group (CD4

T cells cultured under standard conditions)

② Positive activation group (CD4

T cells stimulated with anti‐CD3

anti‐CD28

IL‐6

IL‐23

TGF‐β1

IL‐1β

and IL‐4 to induce differentiation)

and ③ Experimental drug group (positive activation+

Huaiqihuang

treatment). Flow cytometry was used to detect the difference in the expression of CD4

T cell activation markers (CD28 and CD69) and Th17 differentiation marker ROR‐γ. ELISA was employed to measure IL‐17A expression difference. HaCaT cells were cultured

in vitro

and divided into three groups: ① Control group (standard culture conditions)

② Positive activation group (HaCaT cells stimulated with IL‐22 to promote proliferation)

and ③ Experimental drug group (HaCaT cells+IL‐22+

Huaiqihuang

treatment). Western blot was used to assess the difference in JAK2

STAT3

Cyclin D1

and C‐MYC expression.

Results

After

Huaiqihuang

treatment

the expression of CD4

T cell activation markers (CD28 and CD69) was significantly increased (

0.01)

and the expression of Th17 differentiation markers (ROR‐γ and IL‐17A) was significantly reduced (

0.000 1). In HaCaT cells

the experimental drug group exhibited significantly reduced expression of JAK2

p‐JAK2

STAT3

p‐

STAT3

Cyclin D1

and C‐MYC (

0.000 1).

Conclusion

Huaiqihuang

promotes CD4

T cell activation but inhibits their differentiation into Th17 cells by reducing IL‐17A production. Additionally

it suppresses keratinocyte proliferation by modulating the JAK2/STAT3 pathway

providing a novel theoretical basis for its potential application in psoriasis treatment.

关键词

Keywords

references

YANG J F , SUNDRUD M S , SKEPNER J , et al . Targeting Th17 cells in autoimmune diseases [J ] . Trends Pharmacol Sci , 2014 , 35 ( 10 ): 493 ‑ 500 .

HUGH J M , WEINBERG J M . Update on the pathophysiology of psoriasis [J ] . Cutis , 2018 , 102 ( 5S ): 6 ‑ 12 .

YANG G Y , YANG H Q , CUI S F , et al . Effect of huaiqihuang granules combined with comprehensive nursing on children with primary nephrotic syndrome [J ] . J Healthc Eng , 2022 , 2022 ( 1 ): 3279503 .

DAI Y J , ZHAO M , QIU F L , et al . Investigation of the effect of Huaiqihuang granules via adjuvant treatmen t in children with relapsed systemic lupus erythematosus [J ] . Am J Transl Res , 2021 , 13 ( 4 ): 3222 ‑ 3229 .

赵霞 , 王有鹏 , 秦艳虹 , 等 . 儿童哮喘治未病干预方案 [J ] . 南京中医药大学学报 , 2022 , 38 ( 8 ): 676 ‑ 686 .

ZHAO X , WANG Y P , QIN Y H , et al . Intervention program for the preventive treatment of pediatric asthma [J ] . J Nanjing Univ Tradit Chin Med , 2022 , 38 ( 8 ): 676 ‑ 686 .

TSOI L C , SPAIN S L , KNIGHT J , et al . Identification of 15 new psoriasis susceptibility loci highlights the role of innate immunity [J ] . Nat Genet , 2012 , 44 ( 12 ): 1341 ‑ 1348 .

GRIFFITHS C E M , ARMSTRONG A W , GUDJONSSON J E , et al . Psoriasis [J ] . Lancet , 2021 , 397 ( 10281 ): 1301 ‑ 1315 .

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AI问答

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Regulatory effects of Huaiqihuang on the differentiation of CD4+ T cells and HaCaT keratinocytes in vitro

DOI：10.14188/j.1671-8852.2025.0857

摘要

Abstract

关键词

Keywords

references

Regulatory effects of Huaiqihuang on the differentiation of CD4⁺ T cells and HaCaT keratinocytes in vitro