Establishment of obese mouse models by simulating different dietary habits and comparison of their intervention outcomes

GAO Xing; LI Xiaoyue; DENG Ziniu; CHEN Weimin

doi:10.14188/j.1671-8852.2025.0595

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Establishment of obese mouse models by simulating different dietary habits and comparison of their intervention outcomes

更新时间：2026-11-05

- Establishment of obese mouse models by simulating different dietary habits and comparison of their intervention outcomes
- Medical Journal of Wuhan University Vol. 46, Issue 11, Pages: 1406-1412(2025)
- 作者机构：
  
  武汉大学中南医院动物实验中心湖北武汉 430071
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2025.0595
  CLC：
- Received：07 June 2025，
  
  Published：25 November 2025
- 稿件说明：
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高兴,李晓玥,邓子牛等.模拟不同饮食习惯建立肥胖小鼠模型及其干预效果的比较[J].武汉大学学报（医学版）,2025,46(11):1406-1412.

GAO Xing,LI Xiaoyue,DENG Ziniu,et al.Establishment of obese mouse models by simulating different dietary habits and comparison of their intervention outcomes[J].Medical Journal of Wuhan University,2025,46(11):1406-1412.
高兴,李晓玥,邓子牛等.模拟不同饮食习惯建立肥胖小鼠模型及其干预效果的比较[J].武汉大学学报（医学版）,2025,46(11):1406-1412. DOI： 10.14188/j.1671-8852.2025.0595.

GAO Xing,LI Xiaoyue,DENG Ziniu,et al.Establishment of obese mouse models by simulating different dietary habits and comparison of their intervention outcomes[J].Medical Journal of Wuhan University,2025,46(11):1406-1412. DOI： 10.14188/j.1671-8852.2025.0595.

摘要

目的

通过模拟不同饮食习惯建立肥胖小鼠模型，比较其发生机制及干预效果。

方法

42只5周龄雄性C57BL/6小鼠随机分为高糖高脂组（HSHF）、单纯高脂组（HF）和空白对照组（Control），分别饲喂相应饲料12周。每周监测体质量、体长、体宽、血糖，以连续2周Lee指数≥340判定肥胖模型成功。建模后每组处死7只，观察腹腔脂肪蓄积及肝脏油红O染色（评估脂滴沉积）。剩余小鼠改用常规维持饲料并每日运动2 h，干预4周后处死，对比脂肪蓄积程度。

结果

造模期(0~12周)，各组指标随时间增长，实验组与Control组差异逐渐扩大。第12周时，HSHF组体质量(46.98±1.18) g显著高于HF组的体质量[(43.55±1.41) g，

0.01）

]

及Control组的体质量[(36.42±1.12) g，

0.000 1）

]

。HSHF组和HF组均连续2周Lee指数

340且显著高于Control组，标志着肥胖模型建立成功。HSHF组和HF组的血糖亦显著高于Control组。肝脏油红O染色显示实验组脂滴沉积显著增加且范围广泛。干预期(13~16周)，三组体质量、Lee指数及血糖均呈下降趋势。

结论

成功构建肥胖小鼠模型。长期高糖高脂饮食导致体质量、Lee指数、血糖显著升高及脂肪蓄积，证实高热量摄入与肥胖/代谢疾病关联。肝脏病理改变揭示了内脏损伤机制。调整饮食结合规律运动能有效改善肥胖及相关代谢指标，为肥胖相关疾病防治提供了有效干预策略。

Abstract

Objective

To establish obese mouse models by simulating different dietary habits

and compare their mechanisms and intervention effects.

Methods

A total of 42 5‐week‐old male C57BL/6 mice were randomly divided into a high‐sugar‐high‐fat group (HSHF)

a simple high‐fat group (HF)

and a blank control group (Control)

and fed with corresponding feed for 12 weeks. Body mass

body length

body width

and blood glucose were monitored every week

and the success of the obesity model was determined by a Lee index ≥340 for two consecutive weeks. After modeling

7 animals were euthanized in each group to observe intra‐abdominal fat accumulation and liver oil red O staining (to evaluate lipid droplet deposition). The remaining mice were switched to regular maintenance feed and regular exercise for 2 hours daily. After 4 weeks of intervention

they were euthanized and assessed for fat accumulation.

Results

During the modeling period (0‐12 weeks)

the indicators of each group increased over time

and the differences between the experimental groups and the control group gradually widened. At week 12

the body weight of the HSHF group was significantly higher than that of the HF group ([46.98±1.83

]

g versus [43.55±1.41

]

0.01) and the Control group (versus [36.42±1.12

]

0.000 1). The Lee indexes of both the HSHF and HF groups remained above 340 for two consecutive weeks and were significantly higher than those of the Control group

indicating the successful establishment of the obesity models. The blood glucose levels in the experimental groups were also significantly higher than those in the control group. Liver oil red O staining staining showed significantly increased and extensively distributed lipid droplet deposition in the experimental groups. During the intervention period (13‐16 weeks)

the body mass

Lee index

and blood glucose of all three groups showed a decreasing trend.

Conclusion

Two mouse models of obesity were successfully established. Long⁃term feeding of a high⁃glucose⁃high⁃fat diet significantly increased body weight

Lee index

blood glucose

and fat accumulation

confirming the link between excessive caloric intake and obesity/metabolic disorders. The observed hepatic pathological changes revealed the mechanism underlying visceral injury. Dietary modification combined with regular exercise effectively improved obesity and related metabolic parameters

providing an evidence⁃based intervention strategy for prevention and treatment.

关键词

Keywords

references

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