Effect of <italic style="font-style: italic">Ubap2</italic> knockout on spermatogenesis and fertility in mice

DUAN Chaoqun; DENG Xing; SUN Qi; LIU Shaozhi; LUO Mengcheng; ZHOU Jun; PENG Wei; LYU Dan; HAN Yan

doi:10.14188/j.1671-8852.2025.0216

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Effect of Ubap2 knockout on spermatogenesis and fertility in mice

更新时间：2026-11-05

- Effect of Ubap2 knockout on spermatogenesis and fertility in mice
- Medical Journal of Wuhan University Vol. 46, Issue 9, Pages: 1106-1112(2025)
- 作者机构：
  
  1.宜春市妇幼保健院生殖科江西宜春 336000
  2.武汉大学泰康医学院（基础医学院）/泰康生命医学中心发育源性疾病湖北省重点实验室湖北武汉 430060
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2025.0216
  CLC： R339.2
- Received：13 March 2025，
  
  Published：26 September 2025
- 稿件说明：
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段超群,邓星,孙齐等.Ubap2基因敲除对小鼠精子发生及生育能力的影响[J].武汉大学学报（医学版）,2025,46(09):1106-1112.

DUAN Chaoqun,DENG Xing,SUN Qi,et al.Effect of Ubap2 knockout on spermatogenesis and fertility in mice[J].Medical Journal of Wuhan University,2025,46(09):1106-1112.
段超群,邓星,孙齐等.Ubap2基因敲除对小鼠精子发生及生育能力的影响[J].武汉大学学报（医学版）,2025,46(09):1106-1112. DOI： 10.14188/j.1671-8852.2025.0216.

DUAN Chaoqun,DENG Xing,SUN Qi,et al.Effect of Ubap2 knockout on spermatogenesis and fertility in mice[J].Medical Journal of Wuhan University,2025,46(09):1106-1112. DOI： 10.14188/j.1671-8852.2025.0216.

摘要

目的

探究泛素相关蛋白2基因（

Ubap2

）在小鼠精子发生和生育力中的功能。

方法

通过RT⁃qPCR和Western Blot分别检测Ubap2 mRNA和蛋白在小鼠不同天数睾丸组织以及不同组织部位的转录和表达情况；利用CRISPR/Cas9基因编辑技术构建

Ubap2

全身性基因敲除（KO）小鼠，通过Sanger测序和Western Blot进行敲除验证；测量

Ubap2

KO小鼠睾丸大小、睾丸⁃体质量比，检测附睾尾精子数量，结合Diff⁃quick染色评估精子形态学特征。通过连续交配实验检测雄性生育指数，检测

Ubap2

缺失对雄性小鼠生育能力的影响；采用减数分裂染色体铺展技术和免疫荧光染色定位、HE染色、TUNEL凋亡检测分析小鼠

Ubap2

KO对精子发生的各个阶段生精细胞发育的影响。

结果

Ubap2

在睾丸组织中呈现特异性高表达模式；成功构建了

Ubap2

KO小鼠；

Ubap2

KO小鼠未观察到睾丸发育异常，精子发生关键指标及雄性生育力均未显示统计学差异，生精上皮组织学分析显示各阶段生殖细胞比例与对照组无显著差异。

结论

Ubap2

基因对于小鼠精子发生和雄性生育力的维持可能是非必需的。

Abstract

Objective

To investigate the physiological function of ubiquitin⁃associated protein 2 (

Ubap2

) gene in spermatogenesis and fertility in mice.

Methods

The expression of Ubap2 in different days and tissues of mice was detected by RT⁃qPCR and Western Blot;

Ubap2

gene knockout (KO) mouse lines were constructed using CRISPR/Cas9 gene editing technology

and the knockout is verified through Sanger sequencing and Western Blot. Testicular size

testis⁃body weight ratio

sperm number and morphology were analyzed

and the effect of

Ubap2

deletion on male mouse fertility was detected through fertility test experiments. The effect of

Ubap2

KO on the development of spermatogenic cells at various stages of spermatogenesis was analyzed through chromosome spreading

immunofluorescence staining

HE staining and TUNEL apoptosis detection.

Results

Ubap2

is a gene highly expressed in the testis;

Ubap2

KO mice were successfully constructed using CRISPR/Cas9 technology;

Ubap2

deletion in mice had no statistically significant effect on spermatogenic cells

sperm number

sperm morphology and male fertility at all stages of spermatogenesis.

Conclusion

The

Ubap2

gene may be non⁃essential for the maintenance of spermatogenesis and male fertility in mice.

关键词

Keywords

references

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Effect of Ubap2 knockout on spermatogenesis and fertility in mice

DOI：10.14188/j.1671-8852.2025.0216

摘要

Abstract

关键词

Keywords

references