Influence of different anesthesia methods on cognitive function of rats with osteoporosis fracture and their mechanism

XIA Linzhi; YANG Wenchao

doi:10.14188/j.1671-8852.2024.0353

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Influence of different anesthesia methods on cognitive function of rats with osteoporosis fracture and their mechanism

更新时间：2026-11-05

- Influence of different anesthesia methods on cognitive function of rats with osteoporosis fracture and their mechanism
- Medical Journal of Wuhan University Vol. 46, Issue 4, Pages: 469-473(2025)
- 作者机构：
  
  1.武汉中西医结合骨科医院（武汉体育学院附属医院）麻醉科湖北武汉 430070
  2.武汉市第四医院麻醉科湖北武汉 430030
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2024.0353
  CLC： R274.1
- Received：12 May 2024，
  
  Published：25 April 2025
- 稿件说明：
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夏林智,杨文超.不同麻醉方式对骨质疏松骨折大鼠认知功能的影响及其机制[J].武汉大学学报（医学版）,2025,46(04):469-473.

XIA Linzhi,YANG Wenchao.Influence of different anesthesia methods on cognitive function of rats with osteoporosis fracture and their mechanism[J].Medical Journal of Wuhan University,2025,46(04):469-473.
夏林智,杨文超.不同麻醉方式对骨质疏松骨折大鼠认知功能的影响及其机制[J].武汉大学学报（医学版）,2025,46(04):469-473. DOI： 10.14188/j.1671-8852.2024.0353.

XIA Linzhi,YANG Wenchao.Influence of different anesthesia methods on cognitive function of rats with osteoporosis fracture and their mechanism[J].Medical Journal of Wuhan University,2025,46(04):469-473. DOI： 10.14188/j.1671-8852.2024.0353.

摘要

目的

探究不同麻醉方式对骨质疏松骨折模型大鼠认知功能的影响及潜在机制。

方法

实验大鼠分为：对照组、七氟醚组、丙泊酚组及复合麻醉组，每组10只；Morris水迷宫评价大鼠认知功能；尼氏染色观察大鼠神经元细胞数量变化；Western Blot检测大鼠脑海马组织γ‑氨基丁酸A型受体（GABA

）、钾氯联合转运蛋白2（KCC2）蛋白表达。

结果

复合麻醉组较七氟醚组、丙泊酚组在逃避潜伏期上显著降低，在目标象限持续时间、目标象限路程、穿越平台次数上显著升高（

0.05），复合麻醉组大鼠神经元数量较对照组降低但差异无统计学意义（

0.05）；相较于七氟醚组、丙泊酚组，复合麻醉组大鼠神经元数量显著升高（

0.05）；复合麻醉组大鼠GABA

蛋白表达高于七氟醚组、丙泊酚组（

0.05），复合麻醉组大鼠KCC2蛋白表达高于七氟醚组、丙泊酚组但差异无统计学意义（

0.05）。

结论

七氟醚全麻联合丙泊酚局麻的复合麻醉方式较两者单一麻醉方式对骨质疏松骨折术后大鼠认知功能的影响较小，且这一作用可能与上调GABA

、KCC2蛋白表达有关。

Abstract

Objective

To explore the influence of different anesthesia methods on the cognitive function of osteoporosis fracture rat models and their potential mechanisms.

Methods

Experimental rats were divided into four groups: control group

sevoflurane group

propofol group

and combined anesthesia group

with 10 rats in each group. Cognitive function was evaluated using the Morris water maze. Nissl staining was used to observe changes in neuronal cell counts. Protein expression levels of γ‑aminobutyric acid receptor A (GABA

) and potassium‑chloride co‑transporter 2 (KCC2) in the hippocampus were detected using Western blot analysis.

Results

Compared with the sevo

flurane and propofol groups

the escape latency in the combined anesthesia group was significantly shorter

and the duration and distance in the target quadrant

as well as the number of platform crossings

were significantly increased (

0.05). Additionally

the neuron count in the combined anesthesia group was lower than that in the control group

but this difference was not statistically significant (

0.05). The neuron count was significantly higher in the combined anesthesia group than in the sevoflurane and propofol groups (

0.05). GABA

protein expression was also higher in the combined anesthesia group than in the sevoflurane and propofol groups (

0.05). Although the expression of KCC2 protein was higher in the combined anesthesia group than in the sevoflurane and propofol groups

this difference was not statistically significant (

0.05).

Conclusion

The combined anesthesia method of sevoflurane general anesthesia and propofol local anesthesia has a smaller influence on cognitive function after osteoporotic fracture surgery compared to the single anesthesia method. This effect may be related to the upregulation of GABA

and KCC2 protein expressions.

关键词

Keywords

references

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