Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p

ZHAO Yong; DI Huaming; MA Jianwen

doi:10.14188/j.1671-8852.2024.0177

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Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p

更新时间：2026-11-05

- Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p
- Medical Journal of Wuhan University Vol. 46, Issue 9, Pages: 1118-1123(2025)
- 作者机构：
  
  宁夏回族自治区人民医院普通外科宁夏银川 750000
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2024.0177
  CLC： R735.5
- Received：14 March 2024，
  
  Published：26 September 2025
- 稿件说明：
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赵勇,狄华明,马建文.circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制[J].武汉大学学报（医学版）,2025,46(09):1118-1123.

ZHAO Yong,DI Huaming,MA Jianwen.Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p[J].Medical Journal of Wuhan University,2025,46(09):1118-1123.
赵勇,狄华明,马建文.circRHOT1靶向miR‑493‑5p调控结肠癌SW620细胞增殖及凋亡的机制[J].武汉大学学报（医学版）,2025,46(09):1118-1123. DOI： 10.14188/j.1671-8852.2024.0177.

ZHAO Yong,DI Huaming,MA Jianwen.Molecular mechanism of circRHOT1 regulating the proliferation and apoptosis of colon cancer SW620 cells by targeting miR‑493‑5p[J].Medical Journal of Wuhan University,2025,46(09):1118-1123. DOI： 10.14188/j.1671-8852.2024.0177.

摘要

目的

探讨circRHOT1以及miR‑493‑5p对结肠癌细胞生存的影响及它们之间的关系。

方法

本次实验涉及结肠癌细胞SW620以及43对结肠癌组织及癌旁组织。SW620经转染分为si‑NC组、si‑circRHOT1组、miR‑NC组、miR‑493‑5p组、si‑circRHOT1+anti‑miR‑NC组及si‑circRHOT1+anti‑miR‑493‑5p组。采用qRT‑PCR法检测circRHOT1及miR‑493‑5p RNA含量。Western Blot法检测细胞增殖及凋亡相关蛋白表达。CCK‑8以及流式细胞术分别检测SW620细胞增殖及凋亡，双萤光素酶报告实验验证circRHOT1和miR‑493‑5p靶向关系。

结果

结肠癌组织中高表达circRHOT1（

0.05），低表达miR‑493‑5p（

0.05）；敲低SW620细胞中circRHOT1的含量可以降低细胞增殖，促进细胞凋亡（

0.05）；进一步机制分析证实miR‑493‑5p是circRHOT1的功能靶标；miR‑493‑5p过表达抑制细胞增殖，加速细胞凋亡（

0.05）。miR‑493‑5p敲低可以逆转circRHOT1下调诱导的抗增殖以及促凋亡作用（

0.05）。

结论

敲低circRHOT1表达导致miR‑493‑5p高表达，从而抑制结肠癌细胞增殖及诱导细胞凋亡。

Abstract

Objective

To investigate the effects of circRHOT1 and miR‑493‑5p on the survival of colon cancer cells and the relationship between them.

Methods

In this experiment

we utilized the colon cancer cell line SW620 and 43 pairs of colon cancer tissues and adjacent non‑cancerous tissues. The SW620 cells were transfected and divided into the following groups: si‑NC group

si‑circRHOT1 group

miR‑NC group

miR‑493‑5p group

si‑circRHOT1+anti‑miR‑NC group

and si‑circRHOT1+anti‑miR‑493‑5p group. qRT‑PCR was used to measure the circRHOT1 and miR‑493‑5p expression levels. Western Blot was used to detect the expression of proteins related to cell proliferation and apoptosis. The proliferation and apoptosis of SW620 cells were assessed using the CCK‑8 assay and flow cytometry

respectively. The targeting relationship between circRHOT1 and miR‑493‑5p was validated through a dual‑luciferase reporter assay.

Results

circRHOT1 was elevated and miR‑493‑5p was reduced in colon cancer tissues (

0.05). Knocking down of circRHOT1 in SW620 cells could reduce cell proliferation and promote cell apoptosis (

0.05). Further mechanism analysis confirmed that miR‑493‑5p was the functional target of circRHOT1. Overexpression of miR‑493‑5p inhibited cell proliferation and promoted cell apoptosis (

0.05). miR‑493‑5p deletion reversed the anti‑proliferation and pro‑apoptotic effects induced by circRHOT1 downregulation in SW620 cells (

0.05).

Conclusion

Silencing circRHOT1 expression increased the miR‑493‑5p expression level in SW620 cells

thereby inhibiting proliferation and inducing apoptosis.

关键词

Keywords

references

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