Effect of NGALR overexpression on proliferation and apoptosis of renal tubular epithelial cells and its possible mechanism

FANG Xun; CHEN Wenli; ZENG Xingruo; CHEN Longmin; SHEN Jiangman; DONG Yan; LÜ Huijuan; GAO Si

doi:10.14188/j.1671-8852.2024.0128

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Effect of NGALR overexpression on proliferation and apoptosis of renal tubular epithelial cells and its possible mechanism

更新时间：2026-11-05

- Effect of NGALR overexpression on proliferation and apoptosis of renal tubular epithelial cells and its possible mechanism
- Medical Journal of Wuhan University Vol. 46, Issue 5, Pages: 612-618(2025)
- 作者机构：
  
  华中科技大学同济医学院附属武汉中心医院风湿免疫科湖北武汉 430014
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2024.0128
  CLC： R593
- Received：25 February 2024，
  
  Published：25 May 2025
- 稿件说明：
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方珣,陈文莉,曾星若等.NGALR过表达对肾小管上皮细胞增殖和凋亡的影响及其可能机制[J].武汉大学学报（医学版）,2025,46(05):612-618.

FANG Xun,CHEN Wenli,ZENG Xingruo,et al.Effect of NGALR overexpression on proliferation and apoptosis of renal tubular epithelial cells and its possible mechanism[J].Medical Journal of Wuhan University,2025,46(05):612-618.
方珣,陈文莉,曾星若等.NGALR过表达对肾小管上皮细胞增殖和凋亡的影响及其可能机制[J].武汉大学学报（医学版）,2025,46(05):612-618. DOI： 10.14188/j.1671-8852.2024.0128.

FANG Xun,CHEN Wenli,ZENG Xingruo,et al.Effect of NGALR overexpression on proliferation and apoptosis of renal tubular epithelial cells and its possible mechanism[J].Medical Journal of Wuhan University,2025,46(05):612-618. DOI： 10.14188/j.1671-8852.2024.0128.

摘要

目的

探究中性粒细胞明胶酶相关脂质运载蛋白(NGALR)过表达对人肾近曲小管上皮细胞HK⁃2表型、增殖和凋亡的影响及其可能机制。

方法

建立高糖诱导和慢病毒过表达载体，将HK⁃2细胞分为对照组、高糖诱导组、ov⁃NC转染组、ov⁃NGALR组、高糖+ov⁃NGALR组，光镜下观察细胞形态变化，CCK‑8法检测细胞增殖，流式细胞术检测细胞凋亡和周期，Western Blot法检测相关蛋白表达水平。

结果

高糖和/或NGALR过表达使细胞呈长梭形改变，细胞活力显著下降（

0.05），凋亡率显著上升（

0.05），处于G

期的细胞数量显著下降（

0.05），G

/M期的细胞数量显著上升（

0.05），Caspase⁃3、NGALR、NGAL、α⁃SMA、TGF⁃β1蛋白表达水平上升（

0.05）。

结论

与高糖诱导相似，NGALR过表达可改变HK⁃2细胞形态，并且通过改变细胞周期而抑制增殖、促进凋亡，其机制可能与细胞外基质相关因子有关。

Abstract

Objective

To explore the effects of neutro⁃phil gelatinase⁃associated lipocalin(NGALR) overexpression on phenotype

proliferation and apoptosis in human proximal tubular epithelial (HK⁃2) cells

and its possible mechanisms.

Methods

Through high⁃glucose induction and the establishment of lentivirus overexpression vectors

HK⁃2 cells were divided into the control group

high glucose induction group

ov⁃NC transfection group

ov⁃NGALR group

and high glucose+ov⁃NGALR group. Cell morphology changes were observed under light microscope. Cell proliferation was determined by CCK‑8 assay. Apoptosis and cell cycle were determined by flow cytometry. Associated protein expression levels were determined by Western Blot.

Results

High glucose and/or NGALR overexpression caused the cells to change in long spindle shape

the cell viability decreased significantly (

0.05)

the apoptosis rate of cells increased significantly (

0.05)

while the number of cells in the G

phase decreased significantly (

0.05)

the number of cells in G

/M phase increased significantly (

0.05)

and the protein expressions of Caspase⁃3

NGALR

GAL and α⁃SMA increased significantly (

0.05).

Conclusion

Similar to high glucose induction

NGALR overexpression can alter HK⁃2 cell morphology

inhibit proliferation

and promote apoptosis by changing the cell cycle. The mechanism may be related to extracellular matrix⁃related factors.

关键词

Keywords

references

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