Expression of RNF149 in the primary hepatocellular carcinoma and its clinical significance

XU Xu; YUAN Jianhui; DONG Keshuai; YU Jia

doi:10.14188/j.1671-8852.2024.0108

您当前的位置：

首页 >

文章列表页 >

Expression of RNF149 in the primary hepatocellular carcinoma and its clinical significance

更新时间：2026-11-05

- Expression of RNF149 in the primary hepatocellular carcinoma and its clinical significance
- Medical Journal of Wuhan University Vol. 46, Issue 5, Pages: 625-629(2025)
- 作者机构：
  
  武汉大学人民医院肝胆外科湖北武汉 430060
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2024.0108
  CLC： R735.7
- Received：18 February 2024，
  
  Published：25 May 2025
- 稿件说明：
移动端阅览
徐旭,袁建辉,董可帅等.RNF149在原发性肝细胞肝癌中的表达及其临床意义[J].武汉大学学报（医学版）,2025,46(05):625-629.

XU Xu,YUAN Jianhui,DONG Keshuai,et al.Expression of RNF149 in the primary hepatocellular carcinoma and its clinical significance[J].Medical Journal of Wuhan University,2025,46(05):625-629.
徐旭,袁建辉,董可帅等.RNF149在原发性肝细胞肝癌中的表达及其临床意义[J].武汉大学学报（医学版）,2025,46(05):625-629. DOI： 10.14188/j.1671-8852.2024.0108.

XU Xu,YUAN Jianhui,DONG Keshuai,et al.Expression of RNF149 in the primary hepatocellular carcinoma and its clinical significance[J].Medical Journal of Wuhan University,2025,46(05):625-629. DOI： 10.14188/j.1671-8852.2024.0108.

摘要

目的

研究RNF149在原发性肝癌组织和癌旁组织中的表达差异以及其临床意义。

方法

利用实时荧光定量聚合酶链式反应(qPCR)技术来分析RNF149在肝癌组织和癌旁组织的mRNA含量差异。采用免疫组织化学芯片染色来检测肝癌组织和癌旁组织的RNF149蛋白表达差异。同时利用X⁃tile软件和组织芯片85例肝癌数据得到RNF149的最佳截断值0.128，并将组织芯片的肝癌患者分成RNF149高表达组（

=53）和RNF149低表达组(

=32)，然后分析其对临床预后的影响以及与临床病理特征的关系。

结果

RNF149在肝癌组织的mRNA和蛋白表达水平均低于癌旁组织（

0.05和

0.01）；相比于RNF149低表达的肝癌患者，RNF149高表达的患者有更长的总生存期（

=0.045）和无病生存期（

=0.001）。RNF149的高或低表达与患者血清甲胎蛋白（AFP）水平相关（

=0.035），与其他临床病理特征无关（

0.05）。

结论

RNF149在肝细胞肝癌中低表达，高表达的RNF149对肝癌预后具有保护意义。

Abstract

Objective

To study the expression difference of RNF149 in primary liver cancer tissues and paracancerous tissues and its clinical significance.

Methods

Real⁃time fluorescence quantitative polymerase chain reaction (qPCR) was used to analyze the difference in mRNA content between hepatocellular carcinoma tissues and paracancerous tissues of RNF149. Immunohistochemical microarray staining was used to detect the difference in RNF149 protein expression between

hepatocellular carcinoma and paracancerous tissues. Meanwhile

X⁃tile software and tissue microarray data of 85 cases of hepatocellular carcinoma were used to obtain the optimal cutoff value of 0.128 for RNF149

and the hepatocellular carcinoma patients with tissue microarray were divided into the RNF149 high⁃expression group (

=53) and the RNF149 low⁃expression group (

=32). Finally

the effect of RNF149 expression on clinical prognosis and its relationship with clinicopathological features were analyzed.

Results

The mRNA and protein expression levels of RNF149 in hepatocellular carcinoma tissues were lower than that in paracarcinoma tissues (

0.05 and

0.01); compared with hepatocellular carcinoma patients with low expression of RNF149

patients with high expression of RNF149 had longer overall survival (

=0.045) and disease⁃free survival (

=0.001). High and low expression of RNF149 was correlated with the patient's serum α⁃fetoprotein (AFP) levels (

=0.035)

but was not statistically correlated with other clinicopathologic features (

0.05).

Conclusion

The low expression of RNF149 in hepatocellular carcinoma has a protective significance for the prognosis of hepatocellular carcinoma.

关键词

Keywords

references

SUNG H , FERLAY J , SIEGEL R L , et al . Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J ] . CA Cancer J Clin , 2021 , 71 ( 3 ): 209 ⁃ 249 .

WANG J , LIU C Y , HU R H , et al . Statin therapy: A potential adjuvant to immunotherapies in hepatocellular carcinoma [J ] . Front Pharmacol , 2024 , 15 : 1324140 .

LLOVET J M , KELLEY R K , VILLANUEVA A , et al . Hepatocellular carcinoma [J ] . Nat Rev Dis Primers , 2021 , 7 ( 1 ): 6 .

MOSESSON Y , MILLS G B , YARDEN Y . Derailed endocytosis: An emerging feature of cancer [J ] . Nat Rev Cancer , 2008 , 8 ( 11 ): 835 ⁃ 850 .

CAI C M , TANG Y D , ZHAI J B , et al . The RING finger protein family in health and disease [J ] . Signal Transduct Target Ther , 2022 , 7 ( 1 ): 300 .

LIPKOWITZ S , WEISSMAN A M . RINGs of good and evil: Ring finger ubiquitin ligases at the crossroads of tumour suppression and oncogenesis [J ] . Nat Rev Cancer , 2011 , 11 ( 9 ): 629 ⁃ 643 .

QIU X B , GOLDBERG A L . Nrdp1/FLRF is a ubiquitin ligase promoting ubiquitination and degradation of the epidermal growth factor receptor family member, ErbB3 [J ] . Proc Natl Acad Sci USA , 2002 , 99 ( 23 ): 14843 ⁃ 14848 .

WANG H Y , YANG L Q , LIU M H , et al . Protein post⁃translational modifications in the regulation of cancer hallmarks [J ] . Cancer Gene Ther , 2023 , 30 ( 4 ): 529 ⁃ 547 .

REN L , ZHOU T T , WANG Y , et al . RNF8 induces β⁃catenin⁃mediated c⁃Myc expression and promotes colon cancer proliferation [J ] . Int J Biol Sci , 2020 , 16 ( 12 ): 2051 ⁃ 2062 .

KUANG J Y , MIN L , LIU C Y , et al . RNF8 promotes epithelial⁃mesenchymal transition in lung cancer cells via stabilization of slug [J ] . Mol Cancer Res , 2020 , 18 ( 11 ): 1638 ⁃ 1649 .

HOCHSTRASSER M . Ubiquitin, proteasomes, and the regulation of intracellular protein degradation [J ] . Curr Opin Cell Biol , 1995 , 7 ( 2 ): 215 ⁃ 223 .

HERSHKO A . Ubiquitin: Roles in protein modification and breakdown [J ] . Cell , 1983 , 34 ( 1 ): 11 ⁃ 12 .

ZHANG L , CHEN J , YONG J , et al . An essential role of RNF187 in Notch1 mediated metastasis of hepatocellular carcinoma [J ] . J Exp Clin Cancer Res , 2019 , 38 ( 1 ): 384 .

PENG R , ZHANG P F , YANG X , et al . Overexpression of RNF38 facilitates TGF⁃β signaling by ubiquitinating and degrading AHNAK in hepatocellular carcinoma [J ] . J Exp Clin Cancer Res , 2019 , 38 ( 1 ): 113 .

CAI J , XIONG Q , JIANG X , et al . RNF6 facilitates metastasis and radioresistance in hepatocellular carcinoma through ubiquitination of FoxA1 [J ] . Exp Cell Res , 2019 , 374 ( 1 ): 152 ⁃ 161 .

LV B , PAN Y , HOU D , et al . RNF4 silencing induces cell growth arrest and DNA damage by promoting nuclear targeting of p62 in hepatocellular carcinoma [J ] . Oncogene , 2022 , 41 ( 16 ): 2275 ⁃ 2286 .

ZHU J R , TANG J R , WU Y Q , et al . RNF149 confers cisplatin resistance in esophageal squamous cell carcinoma via destabilization of PHLPP2 and activating PI3K/AKT signalling [J ] . Med Oncol , 2023 , 40 ( 10 ): 290 .

YANG H Y , QIN G J , LUO Z , et al . MFSD4A inhibits the malignant progression of nasopharyngeal carcinoma by targeting EPHA2 [J ] . Cell Death Dis , 2022 , 13 ( 4 ): 332 .

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Expression of PIDD and Its Clinical and Pathological Singificance in the Hepatic Cell Carcinoma Tissues

Treatment of Unresectable Primary Hepatocellular Carcinoma by Percutaneous Radiofrequency Ablation Combined with Transcatheter Hepatic Arterial Chemoembolization

Radiofrequency treatment for scalp rejuvenation in facial anti⁃aging

Exploring the differences between CASIA2 and Pentacam in preoperative evaluation of cataracts: A comparison based on measurements of anterior chamber volume, corneal thickness, and pupil diameter

Analysis of risk factors for ICU severe multiple trauma complicated with acute respiratory distress syndrome

Related Author

XU Xu

YU Jia

周猛

王卫星

兰明银

菅志远

严斌

徐旭东

Related Institution

武汉大学人民医院肝胆外科

郧阳医学院附属太和医院普外科

郧阳医学院附属太和医院普外科湖北十堰

武汉大学中南医院普外科

AI问答

⁰