Circular RNA hsa_circ_0117530 promotes cervical cancer cell growth and metastasis through the miR⁃874/ZEB1 axis

ZHENG Chenyang; ZHAO Lingjun; SHEN Qinqin; LÜ Yifan; SHI Huiwei; WANG Yijun

doi:10.14188/j.1671-8852.2023.0675

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Circular RNA hsa_circ_0117530 promotes cervical cancer cell growth and metastasis through the miR⁃874/ZEB1 axis

更新时间：2026-11-05

- Circular RNA hsa_circ_0117530 promotes cervical cancer cell growth and metastasis through the miR⁃874/ZEB1 axis
- Medical Journal of Wuhan University Vol. 46, Issue 3, Pages: 335-342(2025)
- 作者机构：
  
  宁波大学附属妇女儿童医院妇科浙江宁波 315000
- 作者简介：
- 基金信息：
- DOI：10.14188/j.1671-8852.2023.0675
  CLC： R73⁃37
- Received：13 December 2023，
  
  Published：25 March 2025
- 稿件说明：
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郑晨旸,赵玲军,沈亲亲等.环状RNA hsa_circ_0117530通过miR⁃874/ZEB1轴促进宫颈癌细胞生长和转移[J].武汉大学学报（医学版）,2025,46(03):335-342.

ZHENG Chenyang,ZHAO Lingjun,SHEN Qinqin,et al.Circular RNA hsa_circ_0117530 promotes cervical cancer cell growth and metastasis through the miR⁃874/ZEB1 axis[J].Medical Journal of Wuhan University,2025,46(03):335-342.
郑晨旸,赵玲军,沈亲亲等.环状RNA hsa_circ_0117530通过miR⁃874/ZEB1轴促进宫颈癌细胞生长和转移[J].武汉大学学报（医学版）,2025,46(03):335-342. DOI： 10.14188/j.1671-8852.2023.0675.

ZHENG Chenyang,ZHAO Lingjun,SHEN Qinqin,et al.Circular RNA hsa_circ_0117530 promotes cervical cancer cell growth and metastasis through the miR⁃874/ZEB1 axis[J].Medical Journal of Wuhan University,2025,46(03):335-342. DOI： 10.14188/j.1671-8852.2023.0675.

摘要

目的

探讨环状RNA hsa_circ_0117530促进宫颈癌发展的机制。

方法

通过免疫组织化学方法检测ZEB2在宫颈癌组织中的表达，利用circBase和circInteractome数据库进行分析，筛选出环状RNA hsa_circ_0117530。随后，敲低和过表达hsa_circ_0117530，利用CCK⁃8法检测宫颈癌细胞增殖活力。此外，使用CircInteractome数据库预测可能与hsa_circ_0117530结合的miRNA并使用StarBase数据库预测miR⁃874在宫颈癌细胞中的下游靶基因。采用荧光素酶报告基因实验验证并进一步分析其下游的靶点。

结果

证实ZEB2在宫颈癌组织中显著上调，发现hsa_circ_0117530分子源自ZEB2基因，并在宫颈癌组织中的表达显著增高。进一步检测结果显示敲低hsa_circ_0117530抑制了宫颈癌细胞系的生长、增殖和迁移，而过表达则产生相反作用。双荧光素酶报告实验表明hsa_circ_0117530与miR⁃874相互作用，且miR⁃874直接靶向ZEB1基因。

结论

hsa_circ_0117530通过调控miR⁃874/ZEB1轴促进了宫颈癌细胞的生物功能。

Abstract

Objective

To investigate the mechanisms underlying the promotion of cervical cancer development by circular RNA hsa_circ_0117530.

Methods

Immunohistochemistry was used to detect the expression of ZEB2 in cervical cancer tissues. The circBase and circInteractome databases were used to analyze and screen out the circular RNA hsa_circ_0117530. Then

knockdown and overexpression of hsa_circ_0117530 were performed

and the proliferative activity of cervical cancer cells was assessed using the CCK⁃8 assay. In addition

the CircInteractome database was utilized to predict potential miRNAs that could interact with hsa_circ_0117530

and the StarBase database was utilized to predict the downstream target genes of miR⁃874 in cervical cancer cells. Luciferase reporter gene experiments were used for verification

and its downstream targets were further analyzed.

Results

ZEB2 was significantly upregulated in cervical cancer tissues. The circular RNA hsa_circ_0117530 was identified as originating from the ZEB2 gene

and its expression exhibited a significant upregulation in cervical cancer tissues. Further analysis revealed that knockdown of hsa_circ_0117530 inhibited the growth

proliferation

and migration of cervical cancer cell lines

while overexpression had the opposite effect. Luciferase reporter assays confirmed the interaction between hsa_circ_0117530 and miR⁃874

with miR⁃874 directly targeting the ZEB1 gene.

Conclusion

The hsa_circ_0117530 promotes the biological functions of cervical cancer cells by regulating the miR⁃874/ZEB1 axis.

关键词

Keywords

references

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