Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） <italic style="font-style: italic">in vitro</italic>

HAO Xiaolong; WU Zhuoya; PAN Xiaocheng; DAI Yin; ZHOU Jie; WANG Jieru

doi:10.13300/j.cnki.hnlkxb.2026.03.023

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Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） in vitro

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） in vitro
- Journal of Huazhong Agricultural University Vol. 45, Issue 3, Pages: 256-264(2026)
- 作者机构：
  
  1.安徽农业大学动物医学院，合肥 230031
  2.安徽省农业科学院畜牧兽医研究所/安徽省畜禽疫病研究中心/ 安徽省畜禽产品安全重点实验室，合肥 230031
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.03.023
  CLC： S852.65⁺1;S853.7
- Received：07 July 2025，
  
  Published：25 May 2026
- 稿件说明：
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郝小龙，武卓雅，潘孝成，等.二氢丹参酮Ⅰ对猪流行性腹泻病毒的体外抑制作用［J］.华中农业大学学报，2026，45（3）：256‐264.

HAO Xiaolong,WU Zhuoya,PAN Xiaocheng,et al.Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） in vitro[J].Journal of Huazhong Agricultural University,2026,45(03):256-264.
郝小龙，武卓雅，潘孝成，等.二氢丹参酮Ⅰ对猪流行性腹泻病毒的体外抑制作用［J］.华中农业大学学报，2026，45（3）：256‐264. DOI： 10.13300/j.cnki.hnlkxb.2026.03.023.

HAO Xiaolong,WU Zhuoya,PAN Xiaocheng,et al.Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） in vitro[J].Journal of Huazhong Agricultural University,2026,45(03):256-264. DOI： 10.13300/j.cnki.hnlkxb.2026.03.023.

摘要

为探究二氢丹参酮Ⅰ（dihydrotanshinoneⅠ，DiⅠ）在体外对猪流行性腹泻病毒（porcine epidemic diarrhea virus，PEDV）的抑制作用，采用CCK-8法检测不同浓度 DiⅠ对Vero和IPEC-J2细胞活力的影响，通过实时荧光定量PCR、蛋白免疫印迹、间，接免疫荧光、病毒滴度测定等方法评估DiⅠ的抗PEDV活性；并考察不同的给药方式对PEDV复制的影响，采用分子对接方法评估DiⅠ与PEDV M

pro

的相互作用。结果显示，DiⅠ在后续实验浓度下，对Vero和IPEC-J2细胞无明显毒性，DiⅠ能够显著抑制PEDV的基因表达与蛋白合成，且呈现剂量依赖性，全处理、共处理与后处理均可显著抑制PEDV的复制。结果表明，DiⅠ具备抗PEDV活性，能够针对PEDV的入侵、复制、释放等关键阶段发挥抑制作用，可以作为一种潜在的针对PEDV感染的抗病毒治疗药物。

Abstract

The CCK-8 assay was used to detect the effect of different concentrations of dihydrotanshinone Ⅰ（DiⅠ） on the viability of Vero and IPEC-J2 cells to study the inhibitory effect of DiⅠ on porcine epidemic diarrhea virus（PEDV）

in vitro

. Methods including real-time fluorescence quantitative PCR（RT-qPCR）， Western blot， indirect immunofluorescence， and virus titer assay were used to evaluate the anti PEDV activity of DiⅠ. The effects of different administration methods on the replication of PEDV were investigated. Molecular docking method was used to evaluate the interaction between DiⅠ and PEDV M

pro

. The results showed that DiⅠ had no significant toxicity to Vero and IPEC-J2 cells at the concentrations used in subsequent experiments. DiⅠ significantly inhibited the gene expression and protein synthesis of PEDV in a dose-dependent manner. Full-treatment， co-treatment， and post-treatment all significantly inhibited the replication of PEDV. It is indicated that DiⅠ has anti PEDV activity and can exert inhibitory effects on key stages including invasion， replication， and release of PEDV， making it a potential candidate drug against PEDV infection.

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Inhibitory effect of dihydrotanshinoneⅠ on porcine epidemic diarrhea virus （PEDV） in vitro

DOI：10.13300/j.cnki.hnlkxb.2026.03.023

摘要

Abstract

关键词

Keywords

references