Transcriptome of lupine responding to saline-alkaline stress regulated by exogenous NO

SUN Hefei; YANG Siyu; CUI Qiufang

doi:10.13300/j.cnki.hnlkxb.2026.03.022

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Transcriptome of lupine responding to saline-alkaline stress regulated by exogenous NO

Horticulture·Forestry | 更新时间：2026-11-05

- Transcriptome of lupine responding to saline-alkaline stress regulated by exogenous NO
- Journal of Huazhong Agricultural University Vol. 45, Issue 3, Pages: 246-255(2026)
- 作者机构：
  
  1.河南牧业经济学院生命科学学院，郑州 450046
  2.南京农业大学植物保护学院，南京 210095
  3.南京林业大学生态与环境学院，南京 210037
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.03.022
  CLC： S682.19
- Received：06 June 2025，
  
  Published：25 May 2026
- 稿件说明：
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孙荷菲，杨思雨，崔秋芳.外源NO调控羽扇豆响应盐碱胁迫的转录组分析［J］.华中农业大学学报，2026，45（3）：246‐255.

SUN Hefei,YANG Siyu,CUI Qiufang.Transcriptome of lupine responding to saline-alkaline stress regulated by exogenous NO[J].Journal of Huazhong Agricultural University,2026,45(03):246-255.
孙荷菲，杨思雨，崔秋芳.外源NO调控羽扇豆响应盐碱胁迫的转录组分析［J］.华中农业大学学报，2026，45（3）：246‐255. DOI： 10.13300/j.cnki.hnlkxb.2026.03.022.

SUN Hefei,YANG Siyu,CUI Qiufang.Transcriptome of lupine responding to saline-alkaline stress regulated by exogenous NO[J].Journal of Huazhong Agricultural University,2026,45(03):246-255. DOI： 10.13300/j.cnki.hnlkxb.2026.03.022.

摘要

为揭示外源NO调控羽扇豆响应盐碱胁迫的分子机制，利用100 mmol/L NaHCO

和Na

（物质的量比为1∶1）混合溶液模拟盐碱胁迫，硝普钠（sodium nitroprusside，SNP）为外源NO供体。试验设置7个处理，分别为空白对照、盐碱胁迫对照（100 mmol/L盐碱混合溶液）及盐碱胁迫下5个浓度梯度（50、100、150、200、250 μmol/L）的SNP处理，测定植株的可溶性糖含量、蔗糖磷酸合成酶及蔗糖合成酶活性，并对盐碱胁迫条件下最适浓度SNP处理组和对照组材料进行转录组测序。结果显示：外源NO处理提高了盐碱胁迫下羽扇豆幼苗的渗透调节物质可溶性糖含量及蔗糖合成的关键蔗糖合成酶和蔗糖磷酸合成酶活性；外源NO处理调控羽扇豆幼苗糖代谢通路中关键酶基因的表达，β-呋喃果糖苷酶、蔗糖合成酶及β-葡萄糖苷酶基因上调，GDP-甘露糖4，6-脱水酶及1，6-二磷酸果糖醛缩酶（Ⅰ类）基因下调；外源NO调控羽扇豆盐碱胁迫响应的差异转录因子主要包括bHLH、ERF、MYB_related、bZIP和C2H2。以上结果表明，外源NO可通过调控羽扇豆糖代谢通路关键基因及转录因子的表达，提高羽扇豆的耐盐碱性。

Abstract

A mixed solution of 100 mmol/L NaHCO₃ and Na₂CO₃ with molar ratio of 1∶1 and sodium nitroprusside （SNP） as the exogenous NO donor was used to simulate saline-alkali stress to study the molecular mechanism of exogenous nitric oxide （NO） regulating lupine’s response to saline-alkaline stress. 7 treatments including blank control， saline-alkaline stress control （100 mmol/L saline-alkaline mixed solution）， and SNP treatments with 5 concentration gradients （50， 100， 150， 200 and 250 μmol/L） under saline alkali stress were set up. The content of soluble sugar， the activity of sucrose phosphate synthase and sucrose synthase in the plants were measured. The transcriptome of the materials in the group with optimal concentration SNP treatment and the control group under saline-alkaline stress was sequenced. Results showed that exogenous NO increased the content of soluble sugar and enhanced the activity of sucrose synthase and sucrose phosphate synthase involved in synthesizing sucrose in seedlings of lupine under saline alkali stress. Exogenous NO treatment regulated the expression of genes encoding key enzyme in the pathway of sugar metabolism in seedlings of lupine， with upregulation of β-fructofuranosidase， sucrose synthase， and β-glucosidase， and downregulation of GDP mannose 4，6-dehydratase and 1，6-diphosphate fructose aldolase （class Ⅰ）. The differential transcription factors regulated by exogenous NO in response to saline-alkaline stress in lupine mainly included bHLH， ERF， MYB_related，bZIP and C2H2. It is indicated that exogenous NO can enhance the saline-alkaline tolerance of lupine by regulating the expression of key genes and transcription factors in the pathway of sugar metabolism in lupine.

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