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1.华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室,广州 510642
2.广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室,广州 510640
3.中国烟草总公司广东省公司,广州 510610
4.广东烟草梅州市有限公司,梅州 514021
Received:04 July 2025,
Published:25 May 2026
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ZHAO Wanqing,LIN Birun,YANG Qiyun,et al.Sodium cholate induced tobacco TMV resistance and its mechanisms[J].Journal of Huazhong Agricultural University,2026,45(03):215-224.
赵婉晴,林壁润,杨祁云,等.胆酸钠诱导的烟草抗TMV效应及其作用机制[J].华中农业大学学报,2026,45(3):215‐224. DOI: 10.13300/j.cnki.hnlkxb.2026.03.019.
ZHAO Wanqing,LIN Birun,YANG Qiyun,et al.Sodium cholate induced tobacco TMV resistance and its mechanisms[J].Journal of Huazhong Agricultural University,2026,45(03):215-224. DOI: 10.13300/j.cnki.hnlkxb.2026.03.019.
为筛选针对烟草花叶病毒(tobacco mosaic virus,TMV)的新型免疫诱抗剂,采用甘草酸二钾、甘草酸二胺、甘草霜、胆酸钠、铝试剂分别处理烟草;24 h后接种TMV;7 d后进行定量PCR和Weatern-blot检测。结果显示:10 mg/mL的胆酸钠处理烟草,可以有效抑制
TMV
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CP
基因的表达和 CP的积累,其最佳诱导时间是24 h,接种TMV后的第11天进行第2次胆酸钠处理,诱导的TMV抗性可以持续30 d以上。对茉莉酸通路、水杨酸通路和乙烯通路相关基因的表达情况分析显示,胆酸钠诱导的TMV抗性与茉莉酸信号通路密切相关。研究表明,胆酸钠是针对TMV的1种有效的免疫诱抗剂,胆酸钠处理还可以促进烟苗的生长。
Tobacco mosaic virus (TMV) is the most common and significant virus infecting tobacco. Tobacco was treated with potassium glycyrrhizinate, glycyrrhizic acid diamide, glycyrrhetin, sodium cholate, and aluminum reagent to screen novel immune elicitors against TMV. TMV was inoculated after 24 hours of treatment. Quantitative PCR and Western blot were used for detection after 7 days. The results showed that treating tobacco with 10 mg/mL sodium cholate effectively inhibited the expression of TMV gene and the accumulation of TMV-coating protein(CP), with an optimal induction time of 24 hours. A second sodium cholate treatment on the 11th day post-TMV inoculation sustained induced TMV resistance for over 30 days.The results of analyzing the expression of genes related to the JA pathway, SA pathway, and ethylene pathway showed that sodium cholate-induced TMV resistance is closely associated with the JA signaling pathway. It is indicated that sodium cholate is an effective immune elicitor against TMV and promotes the growth of tobacco seedlings.
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