Effects of <italic style="font-style: italic">Spodoptera exigua</italic>，herbivore-damaged amaranth and their volatiles on ontendency behavior of <italic style="font-style: italic">Microplitis pallidipes</italic>

CHEN Yijuan; ZHANG Tongyao; JIANG Jiexian; JI Xiangyun

doi:10.13300/j.cnki.hnlkxb.2026.02.021

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Effects of Spodoptera exigua，herbivore-damaged amaranth and their volatiles on ontendency behavior of Microplitis pallidipes

Plant protection | 更新时间：2026-11-05

- Effects of Spodoptera exigua，herbivore-damaged amaranth and their volatiles on ontendency behavior of Microplitis pallidipes
- Journal of Huazhong Agricultural University Vol. 45, Issue 2, Pages: 199-206(2026)
- 作者机构：
  
  上海市农业科学院生态环境保护研究所/上海市设施园艺技术重点实验室/ 农业农村部东南沿海农业绿色低碳重点实验室，上海 201403
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.02.021
  CLC： S476.3
- Received：04 June 2025，
  
  Published：15 March 2026
- 稿件说明：
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陈义娟，张彤瑶，蒋杰贤，等.甜菜夜蛾与虫害苋菜及其挥发物对淡足侧沟茧蜂趋向行为的影响［J］.华中农业大学学报，2026，45（2）：199‐206.

CHEN Yijuan,ZHANG Tongyao,JIANG Jiexian,et al.Effects of Spodoptera exigua，herbivore-damaged amaranth and their volatiles on ontendency behavior of Microplitis pallidipes[J].Journal of Huazhong Agricultural University,2026,45(02):199-206.
陈义娟，张彤瑶，蒋杰贤，等.甜菜夜蛾与虫害苋菜及其挥发物对淡足侧沟茧蜂趋向行为的影响［J］.华中农业大学学报，2026，45（2）：199‐206. DOI： 10.13300/j.cnki.hnlkxb.2026.02.021.

CHEN Yijuan,ZHANG Tongyao,JIANG Jiexian,et al.Effects of Spodoptera exigua，herbivore-damaged amaranth and their volatiles on ontendency behavior of Microplitis pallidipes[J].Journal of Huazhong Agricultural University,2026,45(02):199-206. DOI： 10.13300/j.cnki.hnlkxb.2026.02.021.

摘要

淡足侧沟茧蜂（

Microplitis pallidipes）

是甜菜夜蛾田间自然种群数量的重要调节因子，为深入探究有效影响该寄生蜂趋向行为的挥发性物质来源与种类，利用“Y”型嗅觉仪分别测定了淡足侧沟茧蜂对甜菜夜蛾幼虫取食后的虫害苋菜、取食苋菜的甜菜夜蛾幼虫及其虫粪的行为反应，并利用顶空固相微萃取法和GC-MS对虫害苋菜、取食苋菜的甜菜夜蛾幼虫及其虫粪释放的挥发性物质进行收集与分析。研究结果显示，虫害苋菜和甜菜夜蛾幼虫对淡足侧沟茧蜂均展现出显著的引诱作用（引诱率分别为67.86%和65.38%），而甜菜夜蛾虫粪的引诱效果不明显。GC-MS结果显示，在虫害苋菜、甜菜夜蛾幼虫和虫粪中分别检测到40、27和32种化合物，主要为烯烃和烷烃类化合物。其中，分别有22、17和11种化合物仅在虫害苋菜、甜菜夜蛾幼虫和虫粪中检测出。另外，在7种共同被检测出的化合物中，（

）-4，8-二甲基-1，3，7-壬三烯（DMNT）、水杨酸甲酯、苯甲酸甲酯在虫害苋菜中的含量高于甜菜夜蛾幼虫和虫粪，而甜菜夜蛾幼虫中的α-雪松烯含量高于虫害苋菜和甜菜夜蛾虫粪。以上研究表明，虫害苋菜和甜菜夜蛾幼虫是苋菜-甜菜夜蛾复合体显著引诱淡足侧沟茧蜂的挥发物的主要来源。

Abstract

Microplitis pallidipes

is a crucial parasitoid wasp regulating natural populations of

Spodoptera exigua

in the field.To clarify the sources and compositions of volatile compounds that influence the host-seeking behavior of this parasitoid，a Y-tube olfactometer assay was conducted to evaluate the behavioral responses of

M. pallidipes

to amaranth damaged by larvae，

S. exigua

larvae feeding on amaranth，and larval excrement.The volatiles emitted by these sources were collected and analyzed using headspace sol

id-phase microextraction （HS-SPME） combined with gas chromatography-mass spectrometry （GC-MS）.The results showed that both larvae-damaged amaranth and

S. exigua

larvae exhibited significant attractiveness to

M. pallidipes

，with attraction rates of 67.86% and 65.38%，respectively，whereas

S. exigua

excrement showed no attraction.GC-MS analysis identified 40，27，and 32 compounds in larvae-damaged amaranth，

S. exigua

larvae，and larval excrement，respectively，with alkenes and alkanes as the primary components.Among these，22，17，and 11 compounds were uniquely detected in the three respective treatments.Additionally，among the seven compounds common to all three sources，the relative levels of （

）-4，8-dimethyl-1，3，7-nonatriene （DMNT），methyl salicylate，and methyl benzoate were higher in larvae-damaged amaranth than in the larvae and their excrements，whereas α-himachalene was more abundant in

S. exigua

larvae compared to the damaged amaranth and excrements.These results indicate that larvae-damaged amaranth and

S. exigua

larvae are the primary sources of volatiles from the amaranth-

S. exigua

complex that significantly attract

M. pallidipes

关键词

Keywords

references

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Effects of Spodoptera exigua，herbivore-damaged amaranth and their volatiles on ontendency behavior of Microplitis pallidipes

DOI：10.13300/j.cnki.hnlkxb.2026.02.021

摘要

Abstract

关键词

Keywords

references