Effects of inoculation quantity of <italic style="font-style: italic">Mesorhizobium huakuii </italic>on nodule formation and nitrogen fixation in<italic style="font-style: italic"> Astragalus sinicus</italic>

WANG Delu; ZHENG Luyao; YANG Guoping; CHEN Dasong; LIN Hui; XIE Fuli; LI Youguo

doi:10.13300/j.cnki.hnlkxb.2026.02.019

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Effects of inoculation quantity of Mesorhizobium huakuii on nodule formation and nitrogen fixation in Astragalus sinicus

Resources and environment | 更新时间：2026-11-05

- Effects of inoculation quantity of Mesorhizobium huakuii on nodule formation and nitrogen fixation in Astragalus sinicus
- Journal of Huazhong Agricultural University Vol. 45, Issue 2, Pages: 183-189(2026)
- 作者机构：
  
  1.华中农业大学农业微生物资源发掘与利用全国重点实验室，武汉430070
  2.宁夏五丰农业科技有限公司，银川750021
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.02.019
  CLC： Q939.114;S154.3
- Received：10 June 2025，
  
  Published：15 March 2026
- 稿件说明：
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王德禄，郑路瑶，杨国平，等.华癸中慢生根瘤菌接种数量对紫云英结瘤固氮的影响［J］.华中农业大学学报，2026，45（2）：183‐189.

WANG Delu,ZHENG Luyao,YANG Guoping,et al.Effects of inoculation quantity of Mesorhizobium huakuii on nodule formation and nitrogen fixation in Astragalus sinicus[J].Journal of Huazhong Agricultural University,2026,45(02):183-189.
王德禄，郑路瑶，杨国平，等.华癸中慢生根瘤菌接种数量对紫云英结瘤固氮的影响［J］.华中农业大学学报，2026，45（2）：183‐189. DOI： 10.13300/j.cnki.hnlkxb.2026.02.019.

WANG Delu,ZHENG Luyao,YANG Guoping,et al.Effects of inoculation quantity of Mesorhizobium huakuii on nodule formation and nitrogen fixation in Astragalus sinicus[J].Journal of Huazhong Agricultural University,2026,45(02):183-189. DOI： 10.13300/j.cnki.hnlkxb.2026.02.019.

摘要

为探究接种华癸中慢生根瘤菌活菌数与紫云英植株结瘤固氮之间的关系，采用种子表面接种和植株根部接种2种方式，以液体菌

剂为接种剂，通过无菌沙培系统，确定紫云英结瘤固氮所需接种根瘤菌的最低活菌数。结果显示，每粒种子表面接种根瘤菌数量达到10

CFU时，植株叶片开始由黄变绿，地上部鲜质量和株高较更低接种量处理显著增加；同时紫云英植株根部发育出具有固氮活性的根瘤，表现出良好的促生效果。对植株根部接种时，单株紫云英仅需满足10

CFU的接种量，即可开始结瘤固氮。无论选择哪种方式接种，单个紫云英植株接种根瘤菌数量达10

CFU时，其结瘤固氮作用、生物量和植株长势均明显优于低接种量组，继续增加接种量后，共生固氮表型趋于稳定。为充分保障根瘤菌接种剂在田间应用后的结瘤固氮作用，综合考虑菌剂施用的经济有效以及土壤条件的复杂性，确定紫云英单株接种10

CFU根瘤菌为华癸中慢生根瘤菌接种的最低活菌数。

Abstract

Two inoculation methods including seed surface and root inoculations were used to study the effects of inoculation quantity of viable rhizobia

Mesorhizobium huakuii

on the nodule formation and nitrogen fixation in

Astragalus sinicus

. The minimum inoculation quantity of rhizobia required for the nodule formation and nitrogen fixation in

A. sinicus

was determined with a sterile sand-pot experiment system using liquid bacterial agents as inoculants. The results showed that the plant leaves started to turn from yellow to green， and the fresh weight and plant height aboveground significantly increased compared to that in treatments with lower inoculation quantity when the rhizobia concentration inoculated on the seed surface reached 10² CFU/seed. Meanwhile， nitrogen-fixing nodules developed on the roots of the

A. sinicus

plants， having a positive effect on promoting growth. When the plant root was inoculated， a single

A. sinicus

plant required only an inoculation quantity of 10

CFU/plant to initiate the nodule formation and nitrogen fixation. When the quantity of rhizobia inoculated on a single

A. sinicus

plant reached 10

CFU， its nodule formation and nitrogen fixation， biomass， and plant growth was significantly better than that in treatments with low inoculation quantity. Further increasing the inoculation quantity stabilized the symbiotic nitrogen fixation. 10

CFU/plant was determined to be the minimum inoculation quantity of

M. huakuii

rhizobia on

A. sinicus

to fully ensure the nodule formation and nitrogen fixation of rhizobia inoculants after application in the field， and take the economic effectiveness of inoculant application and the complexity of soil conditions into account.

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杜光辉，张琳，丁丽，等.紫云英与水稻秸秆联合还田配合氮肥减施对水稻产量及氮素营养平衡的影响［J］.江苏农业学报，2024，40（6）：1012-1019. DU G H，ZHANG L，DING L，et al.Effects of co-incorporation of Chinese milk vetch and rice straw with nitrogen fertilizer reduction on rice yield and nitrogen nutrition balance［J］.Jiangsu journal of agricultural sciences，2024，40（6）：1012-1019 （in Chinese with English abstract） .

YU Y L，XUE L H，YANG L Z.Winter legumes in rice crop rotations reduces nitrogen loss，and improves rice yield and soil nitrogen supply［J］.Agronomy for sustainable development，2014，34（3）：633-640.

陈逸湘，李忠.紫云英根瘤菌剂的应用与生产方法［J］.现代农业科技，2007（24）：138. CHEN Y X，LI Z.Application and production method of Chinese milk vetch rhizobia agent［J］.Modern agricultural science and technology，2007（24）：138 （in Chinese with English abstract） .

WANG H，TANG S，HAN S，et al.Long-term application of Chinese milk vetch return to soil alters phosphorus forms associated with aggregates［J］.Journal of soil science and plant nutrition，2024，24（1）：831-842.

WANG F，CUI H H，HE F W，et al.The green manure （ Astragalus sinicus L.） improved rice yield and quality and changed soil microbial communities of rice in the karst mountains area［J/OL］.Agronomy，2022，12（8）：1851［2025-06-10］.https：//doi.org/10.3390/agronomy12081851.

LEI B N，WANG J，YAO H Y.Ecological and environmental benefits of planting green manure in paddy fields［J/OL］.Agriculture，2022，12（2）：223［2025-06-10］.https：//doi.org/10.3390/agriculture12020223.

WANG Y K，WANG K，LIANG T，et al.Milk vetch returning combined with lime materials alleviates soil cadmium contamination and improves rice quality in soil-rice system［J/OL］.Science of the total environment，2024，951：175770［2025-06-10］.https：//doi.org/10.1016/j.scitotenv.2024.175770.

KEHALIEW A，ASSEFA G，FEKADU D，et al.Milk yield and quality of crossbred dairy cows fed with different levels of vetch（ Vicia dasycarpa ） hay and concentrate on a basal diet of fresh cut napier grass （ Penissetium purpureum ）［J］. Ethiopian journal of agricultural sciences， 2010，21：161-167.

LIU S L，MA Z Y，ZHANG Y，et al. Astragalus sinicus incorporated as green manure for weed control in corn［J/OL］.Frontiers in plant science，2022，13：829421［2025-06-10］.https：//doi.org/10.3389/fpls.2022.829421.

LIU Y J，GUO Z F，SHI H F. Rhizobium symbiosis leads to increased drought tolerance in Chinese milk vetch （ Astragalus sinicus L.）［J/OL］.Agronomy，2022，12（3）：725［2025-06-10］. https：//doi.org/10.3390/agronomy12030725.

丁玉宇.肥-稻-稻模式下紫云英根瘤菌剂拌种试验［J］.中国农技推广，2024，40（2）：86-88. DING Y Y.Seed dressing experiment of Chinese milk vetch rhizobia agent under fertilizer-rice-rice model［J］.China agricultural technology extension，2024，40（2）：86-88 （in Chinese with English abstract） .

BIAN Q，CHENG K，CHEN L，et al.Organic amendments increased Chinese milk vetch symbiotic nitrogen fixation by enriching Mesorhizobium in rhizosphere［J/OL］.Environmental research，2024，252：118923［2025-06-10］.https：//doi.org/10.1016/j.envres.2024.118923.

刘英，郭熙盛，王允青，等.紫云英根瘤菌应用效果研究［J］.安徽农业科学，2012，40（24）：12046-12047. LIU Y，GUO X S，WANG Y Q，et al.Application effects of Rhizobium of Astragalus ［J］.Journal of Anhui agricultural sciences，2012，40（24）：12046-12047 （in Chinese with English abstract） .

MA T T，ZHOU G P，LIU J，et al.Co-incorporating Chinese milk vetch and rice straw increases rice yield by improving nutrient uptake during rice growth［J/OL］.Sustainability，2023，15（16）：12183［2025-06-10］.https：//doi.org/10.3390/su151612183.

ALBAREDA M，RODRÍGUEZ-NAVARRO D N，TEMPRANO F J.Soybean inoculation：dose，N fertilizer supplementation and rhizobia persistence in soil［J］.Field crops research，2009，113（3）：352-356.

WEAVER R W，FREDERICK L R.Effect of inoculum rate on competitive nodulation of Glycine max L.Merrill.I.Greenhouse studies［J］.Agronomy journal，1974，66（2）：229-232.

JI Z J，YAN H，CUI Q G，et al.Competition between rhizobia under different environmental conditions affects the nodulation of a legume［J］.Systematic and applied microbiology，2017，40（2）：114-119.

DEAKER R，ROUGHLEY R J，KENNEDY I R.Legume seed inoculation technology：a review［J］.Soil biology and biochemistry，2004，36（8）：1275-1288.

WEAVER R W，FR EDERICK L R.Effect of inoculum size on nodulation of Glycine max （L.） merril，variety ford［J］.Agronomy journal，1972，64（5）：597-599.

BOGINO P，BANCHIO E，BONFIGLIO C，et al.Competitiveness of a Bradyrhizobium sp.strain in soils containing indigenous rhizobia［J］.Current microbiology，2008，56（1）：66-72.

李佳欢，希娜，漫静，等.苜蓿根瘤菌接种数量与方式对接种效果的影响［J］.草地学报，2022，30（3）：743-749. LI J H，XI N，MAN J，et al.Effects of rhizobial population and inoculation method on the efficiency of alfalfa Rhizobium inoculation［J］.Acta agrestia sinica，2022，30（3）：743-749 （in Chinese with English abstract） .

ALEMAYEHU D，DECHASSA N.Inoculating faba bean seed with Rhizobium bacteria increases the yield of the crop and saves farmers from the cost of applying phosphorus fertilizer［J］.International journal of plant production，2022，16（2）：261-273.

ROY S，LIU W，NANDETY R S，et al.Celebrating 20 years of genetic discoveries in legume nodulation and symbiotic nitrogen fixation［J］.The plant cell，2020，32（1）：15-41.

MADSEN E L，ALEXANDER M.Transport of Rhizobium and Pseudomonas through soil［J］.Soil science society of America journal，1982，46（3）：557-560.

SESSITSCH A，HOWIESON J G，PERRET X et al.Advances in rhizobium research［J］. Critical reviews in plant sciences， 2002， 21（4）：323-378.

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Effects of inoculation quantity of Mesorhizobium huakuii on nodule formation and nitrogen fixation in Astragalus sinicus

DOI：10.13300/j.cnki.hnlkxb.2026.02.019

摘要

Abstract

关键词

Keywords

references