Structural characteristics and gene expression regulation of skin specialization in <italic style="font-style: italic">Pareuchiloglanis anteanalis</italic>

WANG Cong; LI Shunyao; XIONG Xuemei; YANG Ruibin; GAO Zexia; XIONG Hao; ZHAO Heng; WAN Shiming

doi:10.13300/j.cnki.hnlkxb.2026.02.005

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Structural characteristics and gene expression regulation of skin specialization in Pareuchiloglanis anteanalis

Breeding and conservation of endemic fish species in the Jinsha River | 更新时间：2026-11-05

- Structural characteristics and gene expression regulation of skin specialization in Pareuchiloglanis anteanalis
- Journal of Huazhong Agricultural University Vol. 45, Issue 2, Pages: 37-44(2026)
- 作者机构：
  
  1.华中农业大学水产学院/农业农村部淡水生物繁育重点实验室/长江经济带大宗水生生物产业绿色发展教育部工程研究中心，武汉 430070
  2.湖北洪山实验室，武汉 430070
  3.华电金沙江上游水电开发有限公司叶巴滩分公司，甘孜 627153
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.02.005
  CLC： Q959.4;Q785;Q75
- Received：30 May 2025，
  
  Published：15 March 2026
- 稿件说明：
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王聪，李顺尧，熊雪梅，等.前臀鮡上颌和胸鳍特化皮肤组织结构特征和基因表达调控［J］.华中农业大学学报，2026，45（2）：37‐44.

WANG Cong,LI Shunyao,XIONG Xuemei,et al.Structural characteristics and gene expression regulation of skin specialization in Pareuchiloglanis anteanalis[J].Journal of Huazhong Agricultural University,2026,45(02):37-44.
王聪，李顺尧，熊雪梅，等.前臀鮡上颌和胸鳍特化皮肤组织结构特征和基因表达调控［J］.华中农业大学学报，2026，45（2）：37‐44. DOI： 10.13300/j.cnki.hnlkxb.2026.02.005.

WANG Cong,LI Shunyao,XIONG Xuemei,et al.Structural characteristics and gene expression regulation of skin specialization in Pareuchiloglanis anteanalis[J].Journal of Huazhong Agricultural University,2026,45(02):37-44. DOI： 10.13300/j.cnki.hnlkxb.2026.02.005.

摘要

前臀鮡（

Pareuchiloglanis anteanalis

）是长江上游特有的濒危土著鱼类，在长期的高原激流环境条件下，其上颌及鳍条部位皮肤结构发生了特化。为解析前臀鮡特化的调控基础，本研究对其皮肤特化组织结构进行观察，并通过其上颌、胸鳍部位特化的皮肤组织与鳍条部位非特化皮肤组织的转录组比较分析，筛选鉴定皮肤特化相关基因。结果显示，上颌、胸鳍部位特化的皮肤表皮形成密集沟回与感觉芽结构，有助于提升其吸附能力，并且在组织溯源图中产生相邻的聚类，基因表达谱高度相似，同源性高。与非特化皮肤组织的鳍条相比，上颌、胸鳍共筛选到251个共同的差异表达基因，包括146个上调基因和105个下调基因。差异表达基因的KEGG富集分析显示，显著富集通路主要为嘌呤代谢（ko00230）、TGF-β信号通路（ko04350）和钙信号通路（ko04020）。qRT-PCR 验证结果显示，

adcy5

基因在特化皮肤中显著上调表达（

0.05）。综上所述，前臀鮡通过在上颌与胸鳍形成密集沟回和感觉芽结构来提升吸附能力，以适应高原激流环境。该皮肤特化过程主要受细胞突起组装与胶原代谢等关键通路的共同驱动，且

adcy5

基因表达的显著上调表明其在皮肤特化中发挥着潜在调控作用。

Abstract

Pareuchiloglanis anteanalis

is an endangered endemic species native to the upper Yangtze River.To adapt to fast-flowing highland river environment， the skin structures in its upper jaw and fin-ray regions have undergone specialization， providing strong adhesive capabilities.To elucidate the regulatory basis of this specialization， we examined the histological featur

es of the specialized skin， performed comparative transcriptomic analyses between specialized skin （from the upper jaw and pectoral fin） and non-specialized skin （from the fin-ray region）， screened and identified genes associated with skin specialization.The results showed that the specialized epidermis of the upper jaw and pectoral fin develops dense ridges， grooves and sensory bud structures， which enhance adhesion.Tissue-origin mapping showed that these specialized tissues formed adjacent clusters with highly similar gene expression profiles and strong homology.Compared to the non-specialized skin from the fin-ray region， the upper jaw and pectoral fin shared 251 differentially expressed genes （DEGs）， including 146 upregulated and 105 downregulated genes.KEGG enrichment analysis indicated that the significantly enriched pathways were primarily purine metabolism （ko00230）， TGF-β signaling pathway （ko04350）， and calcium signaling pathway （ko04020）.qRT-PCR validation confirmed that

adcy5

was significantly upregulated in the specialized skin （

0.05）.In summary，

P. anteanalis

develops dense epidermal ridge-groove structures and sensory buds in the upper jaw and pectoral fin to enhance adhesion， adapting to the fast-flowing highland river environment.This skin specialization process is primarily driven by key pathways such as cell projection assembly and collagen metabolism， and the significant upregulation of

adcy5

suggests its potential regulatory role in skin specialization.

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references

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Structural characteristics and gene expression regulation of skin specialization in Pareuchiloglanis anteanalis

DOI：10.13300/j.cnki.hnlkxb.2026.02.005

摘要

Abstract

关键词

Keywords

references