Comparative study on sperm ultrastructure and kinematic parameters in <italic style="font-style: italic">Pareuchiloglanis anteanalis</italic> and <italic style="font-style: italic">Pareuchiloglanis gongshanensis</italic>

WANG Hao; XIONG Hao; JIN Yang; FAN Yawen; YANG Xuefen; ZHAO Heng; YANG Ruibin

doi:10.13300/j.cnki.hnlkxb.2026.02.004

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Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis

Breeding and conservation of endemic fish species in the Jinsha River | 更新时间：2026-11-05

- Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis
- Journal of Huazhong Agricultural University Vol. 45, Issue 2, Pages: 28-36(2026)
- 作者机构：
  
  1.华中农业大学水产学院，武汉430070
  2.华电金沙江上游水电开发有限公司叶巴滩分公司，甘孜627153
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.02.004
  CLC： S917
- Received：22 January 2026，
  
  Published：15 March 2026
- 稿件说明：
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汪灏，熊皓，金阳，等.前臀鮡和贡山鮡精子的超微结构与运动学参数比较［J］.华中农业大学学报，2026，45（2）：28‐36.

WANG Hao,XIONG Hao,JIN Yang,et al.Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis[J].Journal of Huazhong Agricultural University,2026,45(02):28-36.
汪灏，熊皓，金阳，等.前臀鮡和贡山鮡精子的超微结构与运动学参数比较［J］.华中农业大学学报，2026，45（2）：28‐36. DOI： 10.13300/j.cnki.hnlkxb.2026.02.004.

WANG Hao,XIONG Hao,JIN Yang,et al.Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis[J].Journal of Huazhong Agricultural University,2026,45(02):28-36. DOI： 10.13300/j.cnki.hnlkxb.2026.02.004.

摘要

为探明前臀鮡（

Pareuchiloglanis anteanalis

）和贡山鮡（

Pareuchiloglanis gongshanensis

）精子的超微结构与运动学特征，采用扫描电镜、透射电镜及计算机辅助精子分析系统（CASA）进行系统比较分析。结果显示，2种鮡精子均由无顶体头部、中段及单鞭毛组成，但在形态构型与运动逻辑上分化显著。前臀鮡精子较长，头部细长（长宽比1.51），中段含4个伞状紧密排列的线粒体，鞭毛具对称侧鳍；贡山鮡精子较短，头部近球形（长宽比1.08），中段含4~5个较小线粒体，鞭毛更短而侧鳍更发达。CASA系统测定结果显示，两者平均路径速率（VAP）、平均直线速率（VSL）和平均曲线速率（VCL）差异不显著，但前臀鮡精子前向性（STR）和鞭打频率（BCF）显著更高（

0.05），呈较直的运动轨迹；贡山鮡精子头部侧摆幅度（ALH）更大（

0.05），运动轨迹更弯曲。研究表明，前臀鮡精子的形态结构与运动学特征高度符合体内授精型精子的典型表型，而贡山鮡精子的形态结构与运动学特征则表现出对体外受精环境的适应。

Abstract

To investigate the sperm ultrastructure and kinematic characteristics of

Pareuchiloglanis anteanalis

and

Pareuchiloglanis gongshanensis

， a systematic comparative analysis was conducted using scanning electron microscopy （SEM）， transmission electron microscopy （TEM）， and a computer-assisted sperm analysis （CASA） system. The results revealed that， although the spermatozoa of both species consist of an acrosome-less head， a midpiece， and a single flagellum， they exhibit significant divergences in morphological configuration and swimming patterns. The spermatozoa of

P. anteanalis

were relatively longer， characterized by a slender head （length-to-width ratio： 1.51）， a midpiece containing four umbrella-shaped， tightly arranged mitochondria， and a flagellum with symmetrical lateral fins. In contrast， the spermatozoa of

P. gongshanensis

were shorter， featuring a nearly spherical head （ratio： 1.08）， a midpiece with 4-5 smaller mitochondria， and a shorter flagellum with more developed lateral fins. CASA measurements revealed no significant differences in average path velocity （VAP）， straight-line velocity （VSL）， or curvilinear velocity （VCL） between the two species. However，

P. anteanalis

sperm exhibited significantly higher straightness （STR） and beat-cross frequency （BCF）（

0.05）， resulting in a more linear swimming trajectory. Conversely，

P. gongshanensis

sperm showed a significantly greater amplitude of lateral head displacement （ALH）（

0.05） and a more curved movement path. This study indicates that

P. anteanalis

sperm exhibit the classic “introsperm” phenotype adapted for internal fertilization， whereas

P. gongshanensis

sperm display traits characteristic of “aquasperm”， reflecting an adaptation to external fertilization.

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references

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Comparative study on sperm ultrastructure and kinematic parameters in Pareuchiloglanis anteanalis and Pareuchiloglanis gongshanensis

DOI：10.13300/j.cnki.hnlkxb.2026.02.004

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Abstract

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