Screening of medium for <italic style="font-style: italic">Ichthyophthirius multifiliis</italic> theronts and effect of adding agarose and EPC cells to medium on their development

XIAO Jieyin; YANG Hao; GU Zemao

doi:10.13300/j.cnki.hnlkxb.2026.01.027

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Screening of medium for Ichthyophthirius multifiliis theronts and effect of adding agarose and EPC cells to medium on their development

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Screening of medium for Ichthyophthirius multifiliis theronts and effect of adding agarose and EPC cells to medium on their development
- Journal of Huazhong Agricultural University Vol. 45, Issue 1, Pages: 300-307(2026)
- 作者机构：
  
  华中农业大学水产学院/湖北省水生动物病害防控工程技术研究中心/ 农业农村部水生动物疫病专业实验室（华中农业大学），武汉430070
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.01.027
  CLC： S941.51⁺4
- Received：24 May 2025，
  
  Published：15 January 2026
- 稿件说明：
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肖杰尹，杨浩，顾泽茂.多子小瓜虫掠食体培养基的筛选及添加琼脂糖和EPC细胞对虫体发育的影响［J］.华中农业大学学报，2026，45（1）：300‐307.

XIAO Jieyin,YANG Hao,GU Zemao.Screening of medium for Ichthyophthirius multifiliis theronts and effect of adding agarose and EPC cells to medium on their development[J].Journal of Huazhong Agricultural University,2026,45(01):300-307.
肖杰尹，杨浩，顾泽茂.多子小瓜虫掠食体培养基的筛选及添加琼脂糖和EPC细胞对虫体发育的影响［J］.华中农业大学学报，2026，45（1）：300‐307. DOI： 10.13300/j.cnki.hnlkxb.2026.01.027.

XIAO Jieyin,YANG Hao,GU Zemao.Screening of medium for Ichthyophthirius multifiliis theronts and effect of adding agarose and EPC cells to medium on their development[J].Journal of Huazhong Agricultural University,2026,45(01):300-307. DOI： 10.13300/j.cnki.hnlkxb.2026.01.027.

摘要

为探究多子小瓜虫（

Ichthyophthirius multifiliis

）掠食体在体外的发育条件，通过比较掠食体在不同培养基中的存活情况获得掠食体培养的最适培养基；并分析在最适培养基中添加鲤上皮瘤（EPC）细胞和琼脂糖（a：琼脂糖在虫体与细胞混合液下层；b：琼脂糖与虫体和细胞混合；c：琼脂糖在虫体和细胞混合液上层）对掠食体发育的影响。结果显示：（1）掠食体在不同培养基中的存活时间为4~6 d，其中在M199培养基中存活时间最长，但培养基中的掠食体无法发育成滋养体；（2）掠食体在添加EPC细胞团的M199培养基中可发育成滋养体，但仅可存活2 d，且滋养体的大小在第2天为（31.32±3.79） µm，无显著生长趋势（

0.05）；（3）掠食体在3种琼脂糖模式中均可发育成滋养体，且存活时间可达3 d。a、b和c 3种模式中滋养体的大小在第3天分别为（37.40±3.99）、（39.51±8.51）和（45.14±10.92） µm，模式c中的滋养体生长最快。结果表明，在M199培养基中添加EPC细胞团和琼脂糖均可促进掠食体发育成滋养体，且在琼脂糖位于虫体和细胞混合液上层的模式下滋养体生长最快。因此，在多子小瓜虫掠食体的体外培养中，建议采用添加EPC细胞团并以琼脂糖覆盖上层的M199培养基，以最优促进其向滋养体发育与生长。

Abstract

Ichthyophthirius multifiliis

is a ciliated protozoan parasite that infects various freshwater fish， causing ichthyophthiriasis. The life cycle of

I. multifiliis

comprises three stages： theront， trophont， and tomont. Theront， the only infective stage， invades the host and transforms into the trophont. This study first compared the survival of theronts in different cell culture media to identify the most suitable medium for

in vitro

culture. Based on these findings， the effects of adding epithelioma papillosum cyprini （EPC） cells and agarose to the culture medium on theront development were investigated using three models：（a）， agarose was positioned in the lower layer beneath the theront and cell mixture；（b）， the agarose mixed

directly with theront and cell； and （c），agarose positioned in the upper layer above the theront and cell mixture. The survival rate and size of

I. multifiliis

were calculated for each condition. The results were as follows：（1） The longest survival time for theronts in medium M199 was up to 6 d. However， theronts were unable to transform into trophonts. （2） The addition of EPC cell aggregates to medium M199 enabled theronts to transformed into trophonts， which survived for 2 d. The size of trophonts was （31.32±3.79） µm on d 2， with no significant growth trend observed. （3） In all three agarose models， theronts was transformed into trophonts and survived for 3 d. The diameters of trophonts in models a， b， and c modes were （37.40±3.99） µm，（39.51±8.51） µm， and （45.14±10.92） µm， respectively， on day 3. In conclusion， the present study demonstrated that adding either EPC cell aggregates or agarose to medium M199 promoted the transformation of theronts into trophonts. However， significant trophonts growth occurred only in the model where the medium was supplemented with both agarose and cells.

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references

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Related Author

XIAO Jieyin

YANG Hao

GU Zemao

Related Institution

College of Fisherie， Huazhong Agricultural University/Hubei Engineering Technology Research Center for Aquatic Animal Diseases Control and Prevention/National Aquatic Animal Diseases Para-Reference Laboratory （HZAU）

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Screening of medium for Ichthyophthirius multifiliis theronts and effect of adding agarose and EPC cells to medium on their development

DOI：10.13300/j.cnki.hnlkxb.2026.01.027

摘要

Abstract

关键词

Keywords

references