Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （<italic style="font-style: italic">Pelteobagrus fulvidraco</italic>） infected with <italic style="font-style: italic">Edwardsiella ictaluri</italic>

TANG Zirui; ZHOU Xu; JIANG Xinxin; LI Zhangping; ZHANG Guirong; MA Xufa; JI Wei; WEI Kaijian

doi:10.13300/j.cnki.hnlkxb.2026.01.024

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Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri
- Journal of Huazhong Agricultural University Vol. 45, Issue 1, Pages: 266-277(2026)
- 作者机构：
  
  1.华中农业大学水产学院/农业农村部淡水生物繁育重点实验室，武汉430070
  2.浙江海洋大学海洋科学与技术学院，舟山 316022
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.01.024
  CLC： Q959.4
- Received：01 June 2025，
  
  Published：15 January 2026
- 稿件说明：
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唐子瑞，周旭，江欣欣，等.黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析［J］.华中农业大学学报，2026，45（1）：266‐277.

TANG Zirui,ZHOU Xu,JIANG Xinxin,et al.Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri[J].Journal of Huazhong Agricultural University,2026,45(01):266-277.
唐子瑞，周旭，江欣欣，等.黄颡鱼感染鲇爱德华氏菌后IL-17受体基因的分子特征及表达分析［J］.华中农业大学学报，2026，45（1）：266‐277. DOI： 10.13300/j.cnki.hnlkxb.2026.01.024.

TANG Zirui,ZHOU Xu,JIANG Xinxin,et al.Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri[J].Journal of Huazhong Agricultural University,2026,45(01):266-277. DOI： 10.13300/j.cnki.hnlkxb.2026.01.024.

摘要

在哺乳动物中，白细胞介素-17受体（interleukin-17 receptor，IL-17R）家族通过介导IL-17细胞因子的信号来调控炎症反应。为探究

IL⁃17R

基因在鱼类对病原体感染的免疫应答中的潜在功能，从黄颡鱼（

Pelteobagrus fulvidraco，Pf

）中克隆了5个

Pf_IL⁃117R

基因（

Pf_IL⁃117RA

至

Pf_IL⁃17RE

）的部分cDNA序列，并对5个基因的序列分子特征、组织表达谱以及鲇爱德华氏菌感染和免疫刺激物刺激后的表达变化进行分析。结果显示，5个

Pf_IL⁃17R

基因的开放阅读框长度依次为2 430（

Pf_IL⁃17RA

）、1 590（

Pf_IL⁃17RB

）、2 106（

Pf_IL⁃17RC

）、2 235（

Pf_IL⁃17RD

）和2 307 bp（

Pf_IL⁃17RE

），分别编码809、529、701、744和768个氨基酸。氨基酸序列分析显示，黄颡鱼5个

Pf_IL⁃17R

基因与其他硬骨鱼类的同源基因序列相似性较高，其中与斑点叉尾鮰同源基因序列相似性最高。实时荧光定量PCR结果显示，5个

Pf_IL⁃17R

基因在健康黄颡鱼组织中呈现不同的mRNA表达谱，但它们在血液、性腺和鳃中的表达量较高。黄颡鱼感染鲇爱德华氏菌后，鳃、皮肤和头肾中5个

Pf_IL⁃17R

基因mRNA表达水平显著上调，而脾脏中

Pf_IL⁃17RB

、

Pf_IL⁃17RC

、

Pf_IL⁃17RD

和

Pf_IL⁃17RE

基因mRNA表达量显著下调。此外，脂多糖、植物血凝素和聚肌胞苷酸刺激可诱导黄颡鱼外周血白细胞中4个

Pf_IL⁃17R

基因（

Pf_IL⁃17RC

除外）mRNA的表达。研究表明，5个

Pf_IL⁃17R

基因可能在黄颡鱼对病原体感染的免疫应答中发挥重要作用。

Abstract

The interleukin-17 receptor （IL-17R） family in mammals regulates inflammatory responses by mediating the signaling of the IL-17 cytokine. Partial cDNA sequences of five

IL⁃17R

genes （

IL⁃17RA

IL⁃17RE

） were cloned from yellow catfish （

Pelteobagrus fulvidraco

，

） to study the potential role of

IL⁃17R

gene in the immu

ne response to pathogenic infection in fish. The molecular characteristics of the sequence，expression profiles of tissue，and changes in expression of five genes in yellow catfish infected with

Edwardsiella ictaluri

and stimulated with immunostimulants were analyzed. The results showed that the open reading frame （ORF） of the five

IL⁃17R

genes including

IL⁃17RA，Pf

IL⁃17RB，Pf

IL⁃17RC，Pf

IL⁃17RD，Pf

IL⁃17RE

was

2 430 bp，1 590 bp，2 106 bp，2 235 bp，and 2 307 bp，encoding 809 amino acids （aa），529 aa，701 aa，744 aa，and 768 aa，respectively. The results of analyzing the sequence of aa showed that the five

IL⁃17R

genes in yellow catfish had high sequence similarity with homologous genes of other teleosts，among which the sequence similarity with homologous genes of channel catfish was the highest. The results of analyses with Real time fluorescence quantitative PCR showed that the five

IL⁃17R

genes exhibited different profiles of mRNA in healthy yellow catfish，but the levels of their expression were higher in the blood，gonads，and gills. The levels of mRNA expression of five

Pf⁃IL⁃17R

genes were significantly upregulated in the gills，skin，and head kidney of yellow catfish infected with

Edwardsiella ictaluri

，while the expression levels of

IL⁃17RB

，

Pf_IL⁃17RC

，

IL⁃17RD

and

IL⁃17RE

mRNAs were significantly down-regulated in the spleen （

0.05 or

0.01）. Moreover，the levels of mRNA expression of four

IL⁃17R

genes （with the exception of

IL⁃17RC

） were induced in the isolated peripheral blood leukocytes （PBLs） of yellow

catfish stimulated with lipopolysaccharide （LPS），phytohemagglutinin （PHA） and polyinosinic-polycytidylic acid （Poly I：C）. It is indicated that the five

IL⁃17R

genes may play important roles in the immune response of yellow catfish to pathogen infection.

关键词

Keywords

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Molecular characterization and expression profile of interleukin-17 receptor genes in yellow catfish （Pelteobagrus fulvidraco） infected with Edwardsiella ictaluri

DOI：10.13300/j.cnki.hnlkxb.2026.01.024

摘要

Abstract

关键词

Keywords

references