Key pathways underlying social genetic effects of residual feed intake in Duroc pigs based on gut metabolomics

ZHOU Wenxuan; ZHAO Zhenjian; CHEN Dong; CUI Shengdi; WANG Junge; CHEN Ziyang; YU Shixin; CHEN Jiamiao; ZHOU Yaoxi; HUANG Runjie; TANG Guoqing

doi:10.13300/j.cnki.hnlkxb.2026.01.022

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Key pathways underlying social genetic effects of residual feed intake in Duroc pigs based on gut metabolomics

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Key pathways underlying social genetic effects of residual feed intake in Duroc pigs based on gut metabolomics
- Journal of Huazhong Agricultural University Vol. 45, Issue 1, Pages: 246-254(2026)
- 作者机构：
  
  四川农业大学动物科技学院/猪禽种业全国重点实验室/农业农村部畜禽生物组学重点实验室/ 四川省畜禽遗传资源发掘与创新利用重点实验室，成都 611130
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2026.01.022
  CLC： S858.28
- Received：27 April 2025，
  
  Published：15 January 2026
- 稿件说明：
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周文譞，赵真坚，陈栋，等.基于肠道代谢组解析杜洛克猪剩余采食量社会遗传效应的关键通路［J］.华中农业大学学报，2026，45（1）：246‐254.

ZHOU Wenxuan,ZHAO Zhenjian,CHEN Dong,et al.Key pathways underlying social genetic effects of residual feed intake in Duroc pigs based on gut metabolomics[J].Journal of Huazhong Agricultural University,2026,45(01):246-254.
周文譞，赵真坚，陈栋，等.基于肠道代谢组解析杜洛克猪剩余采食量社会遗传效应的关键通路［J］.华中农业大学学报，2026，45（1）：246‐254. DOI： 10.13300/j.cnki.hnlkxb.2026.01.022.

ZHOU Wenxuan,ZHAO Zhenjian,CHEN Dong,et al.Key pathways underlying social genetic effects of residual feed intake in Duroc pigs based on gut metabolomics[J].Journal of Huazhong Agricultural University,2026,45(01):246-254. DOI： 10.13300/j.cnki.hnlkxb.2026.01.022.

摘要

为从肠道代谢组层面探究与杜洛克猪剩余采食量社会遗传效应关联的关键代谢物与代谢通路，在分子层面解释猪饲料报酬社会遗传效应，对209头杜洛克猪进行剩余采食量社会遗传效应（residual feed intake-social genetic effects，RFI-SGE）值估计，选取两端极值个体各10头，分为高社会遗传效应组（HRS）与低社会遗传效应组（LRS），采用超高效液相色谱-高分辨质谱（UPLC-Q Exactive）分析20头杜洛克猪回肠和盲肠代谢物组成，利用PLS-DA等方法筛选回肠与盲肠内HRS组和LRS组的显著差异代谢物，并对显著差异代谢物进行KEGG富集分析，获取差异代谢物参与的主要功能通路。液相二级质谱（LC-MS/MS）分析结果显示，在杜洛克猪回肠与盲肠中分别鉴定出1 111个与590个显著差异代谢物，回肠与盲肠共有γ-氨基丁酸（γ-aminobutyric acid，GABA）等13个差异代谢物。KEGG富集分析结果显示，回肠差异代谢物主要富集于丙氨酸、天冬氨酸和谷氨酸代谢、ABC转运蛋白、氨酰-tRNA生物合成等26条通路；盲肠差异代谢物主要富集于色氨酸代谢、不饱和脂肪酸的生物合成、嘌呤代谢等8条通路。结果表明，杜洛克猪剩余采食量的社会遗传效应与肠道差异代谢物（如GABA等）及关键代谢通路（如氨基酸代谢、色氨酸代谢等）显著相关。

Abstract

The social genetic effects-residual feed intake （SGE-RFI） value of 209 Duroc pigs was estimated to study the key metabolites and metabolic pathways associated with the SGE-RFI in Duroc pigs at the level of intestinal metabolome and provide an explanation for the SGE of pig feed reward at the level of molecule. 10 individuals with extreme value at each end were divided into a high social genetic effect group （HRS） and a low social genetic effect group （LRS）. The ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry （UPLC-MS） was used to analyze the content of metabolites from ileal and cecal in 20 Duroc pigs. The Partial least squares-discriminant analysis （PLS-DA） was used to identify significantly different metabolites in the ileum and cecum of Duroc pigs between HRS and LRS groups. The kyoto encyclopedia of genes and genomes （KEGG） enrichment analysis was performed to elucidate the major functional pathways involving these metabolites. The results showed that the 1 111 and 590 significantly different metabolites in the ileum and cecum were identified， with 13 different metabolites including GABA shared between the ileum and cecum. The different metabolites in the ileum were primarily enriched in 26 pathways including alanine， aspartate， and glutamate metabolism， ABC transporters， and aminoacyl-tRNA biosynthesis. The different metabolites in the cecum were mainly associated with 8 pathways including tryptophan metabolism， biosynthesis of unsaturated fatty acids， and purine metabolism. It is indicated that social genetic effects-residual feed intake （SGE-RFI） in Duroc pigs are significantly correlated with different metabolites including GABA in the ileum and cecum and key metabolic pathways including the metabolism of amino acid and tryptophan. It will provide insights into the metabolic mechanisms underlying the social genetic effects of feed efficiency in farming pig.

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