Identification of pathogen causing root rot nematode disease in <italic style="font-style: italic">Curcuma wenyujin</italic> plants grown in Hainan

LI Qianying; JIN Zixue; NI Chunhui; YANG Shuai; LU Yang; CAI Huihui; XU Chunling; XIE Hui

doi:10.13300/j.cnki.hnlkxb.2025.05.017

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Identification of pathogen causing root rot nematode disease in Curcuma wenyujin plants grown in Hainan

Plant protection | 更新时间：2026-11-05

- Identification of pathogen causing root rot nematode disease in Curcuma wenyujin plants grown in Hainan
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 165-178(2025)
- 作者机构：
  
  1.华南农业大学植物保护学院，广州 510642
  2.海南渊源农业科技有限公司，海口 570100
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.017
  CLC： S432.4⁺5
- Received：07 February 2025，
  
  Published：15 September 2025
- 稿件说明：
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黎倩莹，金自学，倪春辉，等.海南温郁金根腐线虫病的病原鉴定［J］.华中农业大学学报，2025，44（5）：165‐178.

LI Qianying,JIN Zixue,NI Chunhui,et al.Identification of pathogen causing root rot nematode disease in Curcuma wenyujin plants grown in Hainan[J].Journal of Huazhong Agricultural University,2025,44(05):165-178.
黎倩莹，金自学，倪春辉，等.海南温郁金根腐线虫病的病原鉴定［J］.华中农业大学学报，2025，44（5）：165‐178. DOI： 10.13300/j.cnki.hnlkxb.2025.05.017.

LI Qianying,JIN Zixue,NI Chunhui,et al.Identification of pathogen causing root rot nematode disease in Curcuma wenyujin plants grown in Hainan[J].Journal of Huazhong Agricultural University,2025,44(05):165-178. DOI： 10.13300/j.cnki.hnlkxb.2025.05.017.

摘要

海南田间的温郁金发生植株枯萎和根腐症状，从这些温郁金根围土壤中发现一种短体线虫。为确定该短体线虫的种类及其是否是导致温郁金植株枯萎和根腐的病原物，采用形态学和分子生物学相结合的方法对温郁金根际的短体线虫进行鉴定，并在对田间发病情况进行系统调查的基础上，根据柯赫氏证病法则，通过室内盆栽接种的方法测定这种线虫对温郁金的致病性。结果显示，该线虫为咖啡短体线虫（

Pratylenchus coffeae

），它能侵染温郁金根组织，在根组织生长发育完成生活史，并进行大量繁殖，导致根和根茎变褐、坏死或腐烂，植株则黄矮、枯萎，根茎产量和品质显著降低。结果表明，咖啡短体线虫是温郁金根腐线虫病的病原物，该病害是一种新病害，在连作地块发病严重。

Abstract

Symptoms of wilting and root rot were observed in

Curcuma wenyujin

plants grown in Hainan fields.A root-lesion nematode，

Pratylenchus

sp.，was detected in the rhizosphere soil of diseased plants.A combined method of morphological and molecular biology was used to identify root-lesion nematodes in the rhizosphere of

C. wenyujin

to determine the type of nematodes and whether it is the pathogen causing wilting and root rot of

C. wenyujin

plants.Based on a systematic investigation of the incidence of diseases in the field，the pathogenicity of this nematode to

C. wenyujin

was confirmed with greenhouse potted inoculation according to Koch’s postulates.The results showed that the nematode was

Pratylenchus coffeae

.It infected the root tissue of

C. wenyujin

，completed its life cycle and reproduced extensively during the growth and development of root tissue，causing the browning，necrosis，or rotting of roots and rhizomes，accompanied by the stunting，yellowing，and wilting of plants，leading to the significant decrease in the yield and quality of root and stem.It is indicated that

Pratylenchus coffeae

is the pathogen of root rot nematode disease in

C. wenyujin

.This disease is a new disease that can occur throughout the entire growth period of

C. wenyujin

，and is severe in fields with continuo

us cropping.

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references

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Identification of pathogen causing root rot nematode disease in Curcuma wenyujin plants grown in Hainan

DOI：10.13300/j.cnki.hnlkxb.2025.05.017

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