Identification of SNPs in <italic style="font-style: italic">mstn</italic> and <italic style="font-style: italic">mtor</italic> gene of <italic style="font-style: italic">Siniperca chuatsi</italic> and their association with growth traits

MIAO Yunliang; FAN Wangyuan; CHEN Boxiang; HE Shan

doi:10.13300/j.cnki.hnlkxb.2025.05.013

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Identification of SNPs in mstn and mtor gene of Siniperca chuatsi and their association with growth traits

Animal science·Veterinary medicine | 更新时间：2026-11-05

- Identification of SNPs in mstn and mtor gene of Siniperca chuatsi and their association with growth traits
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 126-133(2025)
- 作者机构：
  
  1.华中农业大学水产学院/华中农业大学鳜鱼研究中心，武汉430070
  2.广东海大集团股份有限公司，广州 511400
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.013
  CLC： S917.4
- Received：25 May 2024，
  
  Published：15 September 2025
- 稿件说明：
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缪云亮，范旺远，陈柏湘，等.翘嘴鳜mstn和mtor基因SNP位点鉴定及其与生长性状的关联分析［J］.华中农业大学学报，2025，44（5）：126‐133.

MIAO Yunliang,FAN Wangyuan,CHEN Boxiang,et al.Identification of SNPs in mstn and mtor gene of Siniperca chuatsi and their association with growth traits[J].Journal of Huazhong Agricultural University,2025,44(05):126-133.
缪云亮，范旺远，陈柏湘，等.翘嘴鳜mstn和mtor基因SNP位点鉴定及其与生长性状的关联分析［J］.华中农业大学学报，2025，44（5）：126‐133. DOI： 10.13300/j.cnki.hnlkxb.2025.05.013.

MIAO Yunliang,FAN Wangyuan,CHEN Boxiang,et al.Identification of SNPs in mstn and mtor gene of Siniperca chuatsi and their association with growth traits[J].Journal of Huazhong Agricultural University,2025,44(05):126-133. DOI： 10.13300/j.cnki.hnlkxb.2025.05.013.

摘要

为获得与翘嘴鳜（

Siniperca chuatsi

）生长性状相关的分子标记，辅助选育鳜优良品种，基于翘嘴鳜生长基因

mstn

和

mtor

的基因组DNA序列开展单核苷酸多态性（SNP）位点鉴定，并对这些位点进行生长性状的相关性分析。结果显示，2个基因筛查到mstn-in2、mtor-in26和mtor-E11共3个SNP位点，3个SNP位点多态信息含量（PIC）为0.280 8~0.372 9，在贵州和广东群体中均表现为中度多态性水平；标记-性状关联性分析显示：翘嘴鳜贵州群体中，mstn-in2位点为GG基因型的个体，其全长、体长和体质量的平均值均显著高于该位点为CC基因型的个体（

0.05）。翘嘴鳜广东群体中，mstn-in2位点为GG基因型的个体，其全长、体长、体高和体质量的平均值均显著高于该位点为CC基因型的个体（

0.05）；mtor-in26位点为TT基因型的个体，其全长、体质量和肥满度平均值均显著高于该位点为TC基因型和CC基因型的个体（

0.05）。以上结果表明，mstn-in2位点GG基因型和mtor-in26位点TT基因型为生长优势基因型，可将

mstn

基因和

mtor

基因作为翘嘴鳜分子标记辅助育种的重要候选基因。

Abstract

To identify molecular markers associated with growth traits in mandarin fish （

Siniperca chuatsi

） and to facilitate the selective breeding of superior varieties， single nucleotide polymorphism （SNP） loci were screened based on the genomic DNA sequences of the growth-related genes

mstn

and

mtor

.A subsequent correlation analysis between these loci and growth traits revealed the identification of three SNPs： mstn-in2， mtor-in26， and mtor-E11.The polymorphism information content （PIC） for these three SNPs ranged from 0.280 8 to 0.372 9， indicating moderate levels of polymorphism in both the Guizhou and Guangdong populations.Marker-trait association analysis revealed the following findings： In the Guizhou population， individuals with the GG genotype at the mstn-in2 locus exhibited significantly higher mean values for total length， body length， and body mass compared to those with the CC genotype （

0.05）.Similarly， in the Guangdong population， individuals with the GG genotype at the mstn-in2 locus showed significantly higher mean values for total length， body length， body height， and body mass than those with the CC genotype （

0.05）.Furthermore， in the Guangdong population， individuals with the TT genotype at the mtor-in26 locus exhitied significantly higher mean values for total length， body mass， and Fulton’s condition factor compared to those with the TC or CC genotypes （

0.05）.These findings indicate that the GG genotype at the mstn-in2 locus and the TT genotype at the mtor-in26 locus are advantageous for growth.Therefore， the

mstn

and

mtor

genes are proposed as important candidate genes for molecular marker-assisted breeding programs in mandarin fish.

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Identification of SNPs in mstn and mtor gene of Siniperca chuatsi and their association with growth traits

DOI：10.13300/j.cnki.hnlkxb.2025.05.013

摘要

Abstract

关键词

Keywords

references