Effects of melatonin and jasmonic acid on fruit quality of Bingtang sweet orange

CHEN Hui; YANG Junfeng; LUO Shangmin; ZHANG Ling; ZHU Qian; ZHAO Yushuang; CHEN Tong; YAO Maosheng; CHEN Yuewen

doi:10.13300/j.cnki.hnlkxb.2025.05.010

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Effects of melatonin and jasmonic acid on fruit quality of Bingtang sweet orange

Horticulture·Forestry | 更新时间：2026-11-05

- Effects of melatonin and jasmonic acid on fruit quality of Bingtang sweet orange
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 98-106(2025)
- 作者机构：
  
  1.湖南农业大学园艺学院，长沙 410128
  2.怀化市麻阳苗族自治县农业农村局，怀化 419400
  3.怀化市麻阳苗族自治县宋家湾村集体经济合作社，怀化 419400
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.010
  CLC： S666
- Received：05 December 2024，
  
  Published：15 September 2025
- 稿件说明：
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陈慧，杨俊枫，罗尚民，等.褪黑素和茉莉酸对冰糖橙果实品质形成的影响［J］.华中农业大学学报，2025，44（5）：98‐106.

CHEN Hui,YANG Junfeng,LUO Shangmin,et al.Effects of melatonin and jasmonic acid on fruit quality of Bingtang sweet orange[J].Journal of Huazhong Agricultural University,2025,44(05):98-106.
陈慧，杨俊枫，罗尚民，等.褪黑素和茉莉酸对冰糖橙果实品质形成的影响［J］.华中农业大学学报，2025，44（5）：98‐106. DOI： 10.13300/j.cnki.hnlkxb.2025.05.010.

CHEN Hui,YANG Junfeng,LUO Shangmin,et al.Effects of melatonin and jasmonic acid on fruit quality of Bingtang sweet orange[J].Journal of Huazhong Agricultural University,2025,44(05):98-106. DOI： 10.13300/j.cnki.hnlkxb.2025.05.010.

摘要

为探究褪黑素和茉莉酸在冰糖橙果实品质形成中的作用，以‘黔阳’冰糖橙为试验材料，在果实膨大期和成熟前期进行褪黑素（melatonin，MT）和二氢茉莉酸丙酯（prohydrojasmon，PDJ）喷施，测定果实的内在品质，并对柠檬酸积累关键基因

CsAN1

进行表达分析。结果显示：在果实膨大期，MT处理后10、30和50 d，果实可溶性固形物（total soluble solids，TSS）含量分别较对照组降低15.07%、30.48%和19.67%，而柠檬酸含量显著提高，增幅分别为18.35%、19.11%和30.30%；PDJ处理则显著降低柠檬酸含量，处理后10、30和50 d分别下降25.10%、36.14%和34.25%。在果实成熟前期，MT处理后10、25和40 d，柠檬酸含量较对照组分别增加106.19%、84.82%和63.10%；PDJ处理显著提高果实TSS含量，处理后10、25和40 d分别增加15.59%、2.52%和9.83%。MT处理冰糖橙愈伤组织后，柠檬酸含量升高31.06%，

CsAN1

表达量显著提高。以上结果表明，果实膨大期和成熟前期喷施MT可促进果实柠檬酸积累，并显著上调柠檬酸代谢关键基因

CsAN1

；而成熟前期喷施PDJ则能提高果实TSS含量。

Abstract

‘Qianyang’ Bingtang orange was used to study the effects of melatonin （MT） and jasmonic acid （prohydrojasmon，PDJ）.MT and PDJ were sprayed during the fruit expansion stage and the pre-ripening stage to assess the intrinsic quality of the fruits and the expression of

CsAN1

，a key gene for citric acid accumulation.The results showed that during fruit expansion，fruit soluble solids （TSS） content decreased by 15.07%，30.48% and 19.67% at 10，30 and 50 days after MT treatment compared w

ith the control，respectively，while citric acid content was significantly increased by 18.35%，19.11% and 30.30%.PDJ treatment，on the other hand，significantly reduced the citric acid content，which decreased by 25.10%，36.14% and 34.25% at 10，30 and 50 days after treatment，respectively.During the pre-ripening stage，citric acid content increased by 106.19%，84.82% and 63.10% at 10，25 and 40 d after MT treatment compared to the control group，respectively.PDJ treatment significantly increased the fruit TSS content by 15.59%，2.52% and 9.83% at 10，25 and 40 days after treatment.MT treatment of citrus callus resulted in a 31.06% increase in citric acid content accompanied by significantly upregulated

CsAN1

expression.These findings demonstrated that foliar application of MT during both fruit expansion and pre-ripening phases promoted citric acid accumulation and significantly upregulated

CsAN1

，while PDJ application prior to ripening enhanced the TSS content in fruits.

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references

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