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华中农业大学园艺林学学院/果蔬园艺作物种质创新与利用全国重点实验室,武汉 430070
Received:06 August 2024,
Published:15 September 2025
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陈雨馨,罗巧,皮学娜,等.水杨酸甲酯和茉莉酸甲酯对郁金香花香挥发物的影响[J].华中农业大学学报,2025,44(5):88‐97.
CHEN Yuxin,LUO Qiao,PI Xuena,et al.Effects of methyl salicylate and methyl jasmonate on volatile compounds of tulip flowers[J].Journal of Huazhong Agricultural University,2025,44(05):88-97.
陈雨馨,罗巧,皮学娜,等.水杨酸甲酯和茉莉酸甲酯对郁金香花香挥发物的影响[J].华中农业大学学报,2025,44(5):88‐97. DOI: 10.13300/j.cnki.hnlkxb.2025.05.009.
CHEN Yuxin,LUO Qiao,PI Xuena,et al.Effects of methyl salicylate and methyl jasmonate on volatile compounds of tulip flowers[J].Journal of Huazhong Agricultural University,2025,44(05):88-97. DOI: 10.13300/j.cnki.hnlkxb.2025.05.009.
为探究不同浓度的水杨酸甲酯(methyl salicylate,MeSA)和茉莉酸甲酯(methyl jasmonate,MeJA)对郁金香花香挥发物的影响,采用50、200、600 μmol/L MeSA和MeJA喷施郁金香花苞,利用顶空固相微萃取(HS-SPME)和气相色谱质谱联用技术(GC-M
S)对挥发性成分进行测定和分析。结果显示,郁金香花香挥发物的成分主要包括醇类、烷烃类、萜烯类、苯环类、酮类和酯类,其中醇类的总释放量最高;喷施不同浓度MeSA和MeJA,六类化合物含量呈现不同变化趋势,萜烯类化合物释放量在50 μmol/L MeSA或200 μmol/L MeJA处理下增加量尤其明显;4-十二醇、
(E)
-β-罗勒烯和反式-3-己烯-1-醇为郁金香花朵主要挥发物,其中
(E)
-β-罗勒烯在不同浓度MeSA和MeJA处理下释放量均有所增加,50 μmol/L MeSA和200 μmol/L MeJA处理下释放量分别是对照组的10倍和3.3倍。以上结果表明,外源喷施水杨酸甲酯和茉莉酸甲酯对郁金香花香挥发物的释放具有明显的促进作用。
The effects of different concentrations of methyl salicylate (MeSA) and methyl jasmonate (MeJA) on the volatile compounds of tulip flowers were studied. Petals of tulip were sprayed with MeSA and MeJA of 50 μmol/L, 200 μmol/L and 600 μmol/L. The volatile compounds of tulip flowers were determined and analyzed with headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry (GC-MS). The results showed that the volatile compounds of tulip flowers mainly included alcohols, alkanes, terpenes, benzenes, ketones, and esters, with the highest total release of alcohols. The content of the six types of compounds showed different trends of changing after spraying MeSA and MeJA of different concentrations. The release of terpenes increased significantly under the treatment with 50 μmol/L MeSA or 200 μmol/L MeJA. 4-Dodecanol, (
E
)-β-ocimene, and
trans
-3-hexen-1-ol were the main volatile compounds in tulip flowers. Among them, the release of (
E
)-β-ocimene increased under the treatment with different concentrations of MeSA and MeJA. The release of (
E
)-β-ocimene under the treatment with 50 μmol/L MeSA and 200 μmol/L MeJA was 10 times and 3.3 times higher than that of the control group. It is indicated that exogenous spraying of MeJA and MeSA significantly promotes the release of volatile compounds from tulip flowers. It will provide an important theoretical basis for selecting the appropriate concentration of MeJA and MeSA to improve the fragrance of tulip flowers.
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