Identification of manganese tolerant varieties of<italic style="font-style: italic"> Brassica napus</italic> and their tolerance mechanisms

ZHANG Wen; ZHANG Pengchao; YI Botao; SUN Mingzhu; DAI Zhigang; XIE Min; WANG Chuang; XU Fangsen; DING Guangda

doi:10.13300/j.cnki.hnlkxb.2025.05.008

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Identification of manganese tolerant varieties of Brassica napus and their tolerance mechanisms

Accurate evaluation and identification of crop varieties resistant to barrenness and abiotic stress | 更新时间：2026-11-05

- Identification of manganese tolerant varieties of Brassica napus and their tolerance mechanisms
- Journal of Huazhong Agricultural University Vol. 44, Issue 5, Pages: 80-87(2025)
- 作者机构：
  
  1.华中农业大学资源与环境学院/微量元素研究中心/农业农村部长江中下游耕地保育重点实验室，武汉 430070
  2.江西省农业技术推广中心，南昌 330046
  3.湖北省耕地质量与肥料工作总站，武汉 430070
  4.江西省宜春市袁州区农业农村局，宜春 336099
- 作者简介：
- 基金信息：
- DOI：10.13300/j.cnki.hnlkxb.2025.05.008
  CLC： S565.4
- Received：17 July 2025，
  
  Published：15 September 2025
- 稿件说明：
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张雯，张鹏超，易伯涛，等.甘蓝型油菜耐锰毒品种的鉴定及耐性机制解析［J］.华中农业大学学报，2025，44（5）：80‐87.

ZHANG Wen,ZHANG Pengchao,YI Botao,et al.Identification of manganese tolerant varieties of Brassica napus and their tolerance mechanisms[J].Journal of Huazhong Agricultural University,2025,44(05):80-87.
张雯，张鹏超，易伯涛，等.甘蓝型油菜耐锰毒品种的鉴定及耐性机制解析［J］.华中农业大学学报，2025，44（5）：80‐87. DOI： 10.13300/j.cnki.hnlkxb.2025.05.008.

ZHANG Wen,ZHANG Pengchao,YI Botao,et al.Identification of manganese tolerant varieties of Brassica napus and their tolerance mechanisms[J].Journal of Huazhong Agricultural University,2025,44(05):80-87. DOI： 10.13300/j.cnki.hnlkxb.2025.05.008.

摘要

为探究酸性土壤中锰毒胁迫对不同油菜品种影响及油菜耐锰毒机制，以41份甘蓝型油菜品种为材料，通过营养液培养试验分析其在正常锰和锰毒害条件下的表型，计算锰毒处理下的总鲜质量与正常处理下的总鲜质量的比值，鉴定出锰毒耐受品种（manganese toxin tolerant variety ，Mn-T） 427和敏感品种（manganese toxin sensitive variety，Mn-S） 907，并初步解析油菜耐锰毒的生理机制。结果显示，锰毒胁迫显著抑制敏感品种907生长，其地上部鲜质量和光合速率显著下降，叶绿素降解严重，根系生长受到抑制；同时，敏感品种根中活性氧过量积累，表明其抗氧化系统清除效率低下。相比之下，耐受品种427表现出显著优势，其生物量受限小，能通过增加根长和根表面积等优化根系构型以适应锰毒胁迫，叶绿素损失较小，光合作用受锰毒影响小，并具备更强的抗氧化能力。综上，与锰毒敏感品种相比，锰毒耐受品种通过调控根系生长、维持光合作用及增强抗氧化系统以缓解锰毒对其的损伤。

Abstract

To explore the effects of manganese toxicity stress on different rapeseed varieties and the mechanisms of manganese tolerance in rapeseed under acidic soil conditions，41 varieties of

Brassica napus

were used to analyze their phenotypes under normal manganese and manganese toxicity conditions with nutrient solution （300 μmol/L MnCl

） cultivation to study the effects of manganese toxicity in acidic soil on different varieties of

B. napus

and the mechanisms of manganese toxicity tolerance in

B. napus

.The ratio of total fresh mass under manganese toxicity treatment to total fresh mass under normal treatment was calculated.The manganese toxin tolerant variety 427 （Mn-T 427） and the manganese toxin sensitive variety 907 （Mn-S 907） were identified.The physiological mechanism of manganese tolerance in

B. napus

was preliminarily analyzed.The results showed that manganese toxicity stress significantly inhibited the growth of Mn-S 907，resulting in a significant decrease in shroot fresh weight and photosynthetic rate，severe degradation of chlorophyll，and inhibition of root growth.The excessive accumulation of reactive oxygen species （ROS） in the roots of Mn-S 907 indicated a low clearance efficiency of their antioxidant system.In contrast，Mn-T 427 had significant advantages including less limitation of biomass and the ability to optimize root architecture by increasing root length and surface area to adapt to manganese toxicity stress.The loss of chlorophyll was smaller，photosynthesis was less affected by manganese toxicity，and it had stronger antioxidant capacity.Compared with Mn-S 907，Mn-T 427 alleviates the damage caused by manganese toxicity through regulating root growth，maintaining photosynthesis，and enhancing antioxidant systems.It will provide important germplasm resources and a theoretical foundation for breeding

B. napus

with high yield in acidic soils.

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Identification of manganese tolerant varieties of Brassica napus and their tolerance mechanisms

DOI：10.13300/j.cnki.hnlkxb.2025.05.008

摘要

Abstract

关键词

Keywords

references